The goal of our study is to develop and characterize mucoadhesive films with entrapped lysozyme based on gelatin/sodium carboxymethyl cellulose as perspective antimicrobial preparation. Lysozyme in mucoadhesive films retains more than 95 % of its initial activity for 3 years of storage. Different physical-chemical and biochemical characteristics of entrapped enzyme were evaluated, such as film thickness, weight, time of dissolution in water, bioadhesive force, in vitro lysozyme release, pH- and thermoprofiles of hydrolytic activity, effect of γ-sterilization, etc. We have shown that gelatin/sodium carboxymethyl cellulose films have adhesive force on the level of 4380 Pa. Scanning electron microscopy images shows the relative uniformity of the gelatin surface with entrapped lysozyme. Mucoadhesive films with lysozyme have 100% bactericidal effect on the test strain, Staphylococcus aureus ATCC 25923 F - 49 and thus could be considered as a perspective antimicrobial preparation.

SynopsisThe structural behavior of hydrochlorides of poly-Llysine and tetraglycine depends on water vapor pressure. At low relative humidities, structural rearrangements are slow. Water molecules catalyze these structural rearrangements; thus, in tetraglycine hydrochloride, structural transitions are observed at a very low water content of about 1 H,O molecule per 10 peptide residues. Some general aspects of the mechanism of the hydration mobility in peptide structures are discussed.

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Acts Polymeria 42 (f991) Nr. 7 330 VIKHOREVA, (;r.ADYS€IEV Ct. al.: Slrricturc and properties of cliittman c:arbosyrncthgl ct licr References [l] JACOB, I., awl SCHRBDER, 1-1. R.: Chcmicfascrn 30 (1980) [2] V~C L A V~K , M., LACKO, V., and PECHO~, V.: Chem. vldkna [3] MILITKP, J., JANSA, J., and VANI~EK, J.: Proc. Conf. [4] MILITK~, J., JANSA, J., VANI~EK, J., and CAP, J.: Proc. 114, 228. 80 (1980) 149. FIBRICHEM '80, Bratislava, June 1980. Conf. Modified Polymers, Bratislava, June 1979.formation work decreased with increasing V,, but the coefficients of correlation were relatively low ( r w 0.69). All these results agree with t h e results given in [16]. It is evident that i t can be possible to prepare fibres with higher tenacity and lower elongation by increase of the spinning speed. This leads t o higher molecular orientation, too. Influence of the velocify of cooling airAs calculated in [2] a higher rate of cooling air causes a n