Gelatin methacryloyl-alginate core-shell microcapsules as efficient delivery platforms for prevascularized microtissues in endodontic regeneration

Liang, Xi; Xie, Li; Zhang, Qingyuan; Wang, Ge; Zhang, Siyuan; Jiang, Mingyan; Zhang, Ruitao; Yang, Ting; Hu, Xingyu; Yang, Ziyang; Tian, Weidong

doi:10.1016/j.actbio.2022.03.045

Cited by 31 publications

(26 citation statements)

References 58 publications

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“…Previous studies have implanted DPSCs and HUVECs at the rate of 1 : 1 to 3 : 1 for pulp regeneration. 33,34 A preliminary study in our lab showed that DPSCs and HUVECs at the rate of 2 : 1 were suitable for dental pulp regeneration. Therefore, we implanted DPSC-laden microfibers and HUVEC-laden microfibers at a ratio of 2 : 1 for in vivo experiments.…”

Section: Resultsmentioning

confidence: 99%

Vascularized dental pulp regeneration using cell-laden microfiber aggregates

Liang

Liu

et al. 2022

J. Mater. Chem. B

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Section: Resultsmentioning

confidence: 99%

Vascularized dental pulp regeneration using cell-laden microfiber aggregates

Liang

Liu

et al. 2022

J. Mater. Chem. B

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“…Pure alginate was found to demonstrate the least degree of degradation with cell survival rates in RGD-alginate microspheres exceeding 90%. A similar study was conducted by Liang et al [ 28 ] in fabricating gelatine methacryloyl–alginate core-shell microcapsules for endodontic regeneration that co-encapsulate human dental pulp stem cells and human umbilical vein endothelial cells. The results indicated that human umbilical vein endothelial cells and human dental pulp stem cells microcapsules showed greater cell proliferation rates with the formation of capillary- and odontogenic-like networks, respectively.…”

Section: Resultsmentioning

confidence: 76%

“…All included articles were published within the past ten years except for one published in the year 2005 [ 25 ]. The included articles were either in vitro, in vivo, or ex vivo experimental studies, with the bulk of the research derived from Asiatic countries [ 5 , 6 , 12 , 22 , 23 , 24 , 27 , 28 , 29 ]. Four themes were categorised: endodontic regeneration, intracanal medicament, root filling material, and chelating agent ( Figure 2 ).…”

Section: Resultsmentioning

confidence: 99%

“…During the initial search, a total of 1491 articles were identified, of which 689 duplicate articles were eliminated, followed by the exclusion of another 771 articles after a preliminary screening based on the title and abstract. An in-depth, full-text examination of the remaining 31 articles was performed, and 13 articles were finally selected for qualitative analysis [ 5 , 6 , 12 , 13 , 14 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ]. Figure 1 depicts the flow of study selection and reasons for excluding the articles, whereas Table 1 summarises the characteristics of the included studies.…”

Section: Resultsmentioning

confidence: 99%

“…Hence, it can be anticipated that future studies may benefit from combining alginate with a hyaluronic acid hydrogel to increase cell survival in regenerative endodontics [ 26 ]. On the bright side, drug delivery microcapsules made of this biomaterial may easily be cultivated in standard cell culture plates since alginate hydrogels lack the capacity of cell adherence, which prevents cell-laden microcapsule aggregation [ 28 ]. Notwithstanding, future research should explore ways to shorten the time required for fabrication processes and microsphere delivery systems that tend to aggregate during culture.…”

Section: Discussionmentioning

confidence: 99%

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An Insight into the Role of Marine Biopolymer Alginate in Endodontics: A Review

et al. 2022

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Alginate is a natural marine biopolymer that has been widely used in biomedical applications, but research on its use as an endodontic material is still sparse in the literature. This pioneer review aims to summarize the emerging roles of alginate and to outline its prospective applications as a core biomaterial in endodontics. Ten electronic databases and five textbooks were used to perform a search of English-language literature on the use of alginate in endodontics published between January 1980 and June 2022. The risk of bias (RoB) of each included study was assessed using the Office of Health Assessment and Translation (OHAT) tool. Subsequently, studies were categorized into three tiers to represent the overall risk. Qualitative analysis was performed, and the articles were sorted into different thematic categories. An initial search yielded a total of 1491 articles, but only 13 articles were chosen. For most domains, all the studies were rated with ‘probably low’ or ‘definitely low’ RoB, except for domains 2 and 6. All included studies fall in the Tier 1 category and were either in vitro, in vivo, or ex vivo. Four thematic categories were identified: endodontic regeneration, intracanal medicament, filing material, and chelating agent. Based on the available evidence, alginate has emerged as a cell carrier and scaffold in regenerative endodontics, a microcapsule delivery system for intracanal medicaments, a chelating agent reinforcing material, and a root canal sealer. More well-designed experiments and clinical trials are needed to warrant the promising advent of this hydrogel-based biomaterial.

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Biomimetic Peridontium Patches for Functional Periodontal Regeneration

Yang

Chen

et al. 2023

Adv Healthcare Materials

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The unique structure of the periodontium, including the alveolar bone, cementum, and periodontal ligament (PDL), presents difficulties for the regeneration of its intricate organization. Irreversible structural breakdown of the periodontium increases the risk of tooth loosening and loss. Although the current therapies can restore the periodontal hard tissues to a certain extent, the PDL with its high directionality of multiple groups with different orientations and functions cannot be reconstructed. Here, biomimetic peridontium patches (BPPs) for functional periodontal regeneration using a microscale continuous digital light projection bioprinting method is reported. Orthotopic transplantation in the mandibles shows effective periodontal reconstruction. The resulting bioengineered tissues closely resembles natural periodontium in terms of the "sandwich structures," especially the correctly oriented fibers, showing different and specific orientation in different regions of the tooth root, which has never been found in previous studies. Furthermore, after the assessment of clinically functional properties it is found that the regenerative periodontium can achieve stable tooth movement under orthodontic migration force with no adverse consequences. Overall, the BPPs promote reconstruction of the functional periodontium and the complex microstructure of the periodontal tissue, providing a proof of principle for the clinical functional treatment of periodontal defects.

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Gelatin methacryloyl-alginate core-shell microcapsules as efficient delivery platforms for prevascularized microtissues in endodontic regeneration

Cited by 31 publications

References 58 publications

Vascularized dental pulp regeneration using cell-laden microfiber aggregates

Vascularized dental pulp regeneration using cell-laden microfiber aggregates

An Insight into the Role of Marine Biopolymer Alginate in Endodontics: A Review

Biomimetic Peridontium Patches for Functional Periodontal Regeneration

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