Gelatin Microgel Incorporated Poly(ethylene glycol)-Based Bioadhesive with Enhanced Adhesive Property and Bioactivity

Li, Yuting; Meng, Hao; Liu, Yuan; Narkar, Ameya; Lee, Bruce P.

doi:10.1021/acsami.6b01364

Cited by 89 publications

(63 citation statements)

References 62 publications

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“…11). 207 Gelatin is the denatured form of collagen often used for biomedical applications 208. Gelatin contains nucleophilic side chains (i.e., —NH 2 and —SH from lysine and cysteine, respectively) that enabled gelatin microparticles to be chemically incorporated into the PEG network through covalent bond formation with oxidized catechol (i.e., quinone) found on dopamine [Fig.…”

Section: Recent Polymer Systems Incorporating Novel Biomimetic Designsmentioning

confidence: 99%

Recent approaches in designing bioadhesive materials inspired by mussel adhesive protein

Forooshani

Lee

2016

J. Polym. Sci. Part A: Polym. Chem.

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534

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Marine mussels secret protein‐based adhesives, which enable them to anchor to various surfaces in a saline, intertidal zone. Mussel foot proteins (Mfps) contain a large abundance of a unique, catecholic amino acid, Dopa, in their protein sequences. Catechol offers robust and durable adhesion to various substrate surfaces and contributes to the curing of the adhesive plaques. In this article, we review the unique features and the key functionalities of Mfps, catechol chemistry, and strategies for preparing catechol‐functionalized polymers. Specifically, we reviewed recent findings on the contributions of various features of Mfps on interfacial binding, which include coacervate formation, surface drying properties, control of the oxidation state of catechol, among other features. We also summarized recent developments in designing advanced biomimetic materials including coacervate‐forming adhesives, mechanically improved nano‐ and micro‐composite adhesive hydrogels, as well as smart and self‐healing materials. Finally, we review the applications of catechol‐functionalized materials for the use as biomedical adhesives, therapeutic applications, and antifouling coatings. © 2016 The Authors. Journal of Polymer Science Part A: Polymer Chemistry Published by Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2017, 55, 9–33

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Section: Recent Polymer Systems Incorporating Novel Biomimetic Designsmentioning

confidence: 99%

Recent approaches in designing bioadhesive materials inspired by mussel adhesive protein

Forooshani

Lee

2016

J. Polym. Sci. Part A: Polym. Chem.

Self Cite

534

464

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“…7,83 Furthermore, gelatin is cytocompatible and shows lower antigenicity compared to collagen. 84 At room temperature, gelatin solidifies due to its extensive physical bonds. However, it liquefies upon heating to above the physiological temperature of 37 °C, making it impractical to use gelatin directly as an injectable in situ curable gel.…”

Section: Gel Materialsmentioning

confidence: 99%

Cell-laden microfluidic microgels for tissue regeneration

et al. 2016

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Regenerating the diseased tissue is one of the foremost concerns for the millions of patients who suffer from tissue damage each year. Local delivery of cell-laden hydrogels offers an attractive approach for tissue repair. However, due to the typical macroscopic size of these cell constructs, the encapsulated cells often suffer from poor nutrient exchange. These issues can be mitigated by incorporating cells into microscopic hydrogels, or microgels, whose large surface-to-volume ratio promotes efficient mass transport and enhanced cell-matrix interactions. Using microfluidic technology, monodisperse cell-laden microgels with tunable sizes can be generated in a high-throughput manner, making them useful building blocks that can be assembled into tissue constructs with spatially controlled physicochemical properties. In this review, we examine microfluidics-generated cell-laden microgels for tissue regeneration applications. We provide a brief overview of the common biomaterials, gelation mechanisms, and microfluidic device designs that are used to generate these microgels, and summarize the most recent works on how they are applied to tissue regeneration. Finally, we discuss future applications of microfluidic cell-laden microgels as well as existing challenges that should be resolved to stimulate their clinical application.

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“…Specifically, the use of IO 4 − -mediated crosslinking is widely adopted for designing rapidly curable biomaterials [6, 9, 14]. Mussel inspired bioadhesives have exhibited favorable biocompatibility based on in vitro cytotoxicity and in vivo implantation testing [6, 13, 15]. However, these biocompatibility tests were conducted using fully cured adhesives and the potential toxic byproducts generated during the curing process were not examined.…”

Section: Introductionmentioning

confidence: 99%

Model polymer system for investigating the generation of hydrogen peroxide and its biological responses during the crosslinking of mussel adhesive moiety

2017

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Mussel adhesive moiety, catechol, has been utilized to design a wide variety of biomaterials. However, the biocompatibility and biological responses associated with the byproducts generated during the curing process of catechol has never been characterized. An in situ curable polymer model system, 4-armed polyethylene glycol polymer end-capped with dopamine (PEG-D4), was used to characterize the production of hydrogen peroxide (H2O2) during the oxidative crosslinking of catechol. Although PEG-D4 cured rapidly (under 30 seconds), catechol continues to polymerize over several hours to form a more densely crosslinked network over time. PEG-D4 hydrogels were examined at two different time points; 5 min and 16 hrs after initiation of crosslinking. Catechol in the 5 min-cured PEG-D4 retained the ability to continue to crosslink and generated an order of magnitude higher H2O2 (40 μM) over 6 hrs when compared to 16 hrs-cured samples that ceased to crosslink. H2O2 generated during catechol crosslinking exhibited localized cytotoxicity in culture and upregulated the expression of an antioxidant enzyme, peroxiredoxin 2, in primary dermal and tendon fibroblasts. Subcutaneous implantation study indicated that H2O2 released during oxidative crosslinking of PEG-D4 hydrogel promoted superoxide generation, macrophage recruitment, and M2 macrophage polarization in tissues surrounding the implant. Given the multitude of biological responses associated with H2O2, it is important to monitor and tailor the production of H2O2 generated from catechol-containing biomaterials for a given application.

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Gelatin Microgel Incorporated Poly(ethylene glycol)-Based Bioadhesive with Enhanced Adhesive Property and Bioactivity

Cited by 89 publications

References 62 publications

Recent approaches in designing bioadhesive materials inspired by mussel adhesive protein

Recent approaches in designing bioadhesive materials inspired by mussel adhesive protein

Cell-laden microfluidic microgels for tissue regeneration

Model polymer system for investigating the generation of hydrogen peroxide and its biological responses during the crosslinking of mussel adhesive moiety

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