Gelatin solution pH and incubation time influences the size of the nanoparticles engineered by desolvation

Vinjamuri, Bhavani Prasad; Papachrisanthou, Katarina; Haware, Rahul V.; Chougule, Mahavir B.

doi:10.1016/j.jddst.2021.102423

Cited by 14 publications

(14 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The effect of several factors on the desolvation process was not studied: temperature of starting materials [ 49 ], salt concentration, acidic pH values, gelatin pre-incubation [ 84 ], the longevity of incubation with alcohols, and so on. Nevertheless, we performed preliminary experiments adding NaCl before desolvation.…”

Section: Resultsmentioning

confidence: 99%

Modified Desolvation Method Enables Simple One-Step Synthesis of Gelatin Nanoparticles from Different Gelatin Types with Any Bloom Values

et al. 2021

View full text Add to dashboard Cite

Gelatin nanoparticles found numerous applications in drug delivery, bioimaging, immunotherapy, and vaccine development as well as in biotechnology and food science. Synthesis of gelatin nanoparticles is usually made by a two-step desolvation method, which, despite providing stable and homogeneous nanoparticles, has many limitations, namely complex procedure, low yields, and poor reproducibility of the first desolvation step. Herein, we present a modified one-step desolvation method, which enables the quick, simple, and reproducible synthesis of gelatin nanoparticles. Using the proposed method one can prepare gelatin nanoparticles from any type of gelatin with any bloom number, even with the lowest ones, which remains unattainable for the traditional two-step technique. The method relies on quick one-time addition of poor solvent (preferably isopropyl alcohol) to gelatin solution in the absence of stirring. We applied the modified desolvation method to synthesize nanoparticles from porcine, bovine, and fish gelatin with bloom values from 62 to 225 on the hundreds-of-milligram scale. Synthesized nanoparticles had average diameters between 130 and 190 nm and narrow size distribution. Yields of synthesis were 62–82% and can be further increased. Gelatin nanoparticles have good colloidal stability and withstand autoclaving. Moreover, they were non-toxic to human immune cells.

show abstract

Section: Resultsmentioning

confidence: 99%

Modified Desolvation Method Enables Simple One-Step Synthesis of Gelatin Nanoparticles from Different Gelatin Types with Any Bloom Values

et al. 2021

View full text Add to dashboard Cite

show abstract

“…4. The effect of several factors on the desolvation process was not studied: temperature of starting materials (Azarmi, 2006), salt concentration, acidic pH values, gelatin preincubation (Vinjamuri, 2021), the longevity of incubation with alcohols, and so on. Nevertheless, we performed preliminary experiments adding NaCl before desolvation.…”

Section: Discussionmentioning

confidence: 99%

Modified desolvation method enables simple one-step synthesis of gelatin nanoparticles from different gelatin types with any bloom values

Khramtsov

Burdina

Lazarev

et al. 2021

Preprint

View full text Add to dashboard Cite

Gelatin nanoparticles found numerous applications in drug delivery, bioimaging, immunotherapy, and vaccine development as well as in biotechnology and food science. Synthesis of gelatin nanoparticles is usually made by a two-step desolvation method, which, despite providing stable and homogeneous nanoparticles, has many limitations, namely complex procedure, low yields, and poor reproducibility of the first desolvation step. Herein, we present a modified one-step desolvation method, which enables the quick, simple, and reproducible synthesis of gelatin nanoparticles. Using the proposed method one can prepare gelatin nanoparticles from any type of gelatin with any bloom number, even with the lowest ones, which remains unattainable for the traditional two-step technique. The method relies on quick one-time addition of poor solvent (preferably isopropyl alcohol) to gelatin solution in the absence of stirring. We applied the modified desolvation method to synthesize nanoparticles from porcine, bovine, and fish with bloom values from 62 to 225 on the hundreds-of-milligram scale. Synthesized nanoparticles had average diameters between 130 and 190 nm and narrow size distribution. Yields of synthesis were 62-82% and can be further increased. Gelatin nanoparticles have good colloidal stability and withstand autoclaving. Moreover, they were non-toxic to human immune cells.

show abstract

“…Type B gelatin (5% p/v) was dissolved in type II water at 50 °C by magnetic stirring. Then, acetone at a volume ratio of 1:1 was dripped to the mixture and left still for 5 min to dehydrate gelatin and induce coiling by the prevalence of positive charges in protonated amine (–NH2) functional groups [ 27 , 28 ]. The supernatant, rich in acetone, was collected and centrifuged for 3 min at 4500 RPM to recover the remaining high molecular weight (HMW) gelatin.…”

Section: Methodsmentioning

confidence: 99%

“…Then, the remaining pellet was diluted in type II water (volume ratio of 1:1 with acetone) and reincorporated to the coacervate phase by mixing at 50 °C for 20 min. The pH was adjusted between 10.5–11.5 with 0.1 M NaOH to ionize the pendant carboxyl (–COOH) functional groups of acidic amino acids (i.e., glutamic and aspartic acids) [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

Highly Efficient Synthesis of Type B Gelatin and Low Molecular Weight Chitosan Nanoparticles: Potential Applications as Bioactive Molecule Carriers and Cell-Penetrating Agents

et al. 2021

View full text Add to dashboard Cite

Gelatin and chitosan nanoparticles have been widely used in pharmaceutical, biomedical, and nanofood applications due to their high biocompatibility and biodegradability. This study proposed a highly efficient synthesis method for type B gelatin and low-molecular-weight (LMW) chitosan nanoparticles. Gelatin nanoparticles (GNPs) were synthesized by the double desolvation method and the chitosan nanoparticles (CNPs) by the ionic gelation method. The sizes of the obtained CNPs and GNPs (373 ± 71 nm and 244 ± 67 nm, respectively) and zeta potential (+36.60 ± 3.25 mV and −13.42 ± 1.16 mV, respectively) were determined via dynamic light scattering. Morphology and size were verified utilizing SEM and TEM images. Finally, their biocompatibility was tested to assure their potential applicability as bioactive molecule carriers and cell-penetrating agents.

show abstract

Gelatin solution pH and incubation time influences the size of the nanoparticles engineered by desolvation

Cited by 14 publications

References 24 publications

Modified Desolvation Method Enables Simple One-Step Synthesis of Gelatin Nanoparticles from Different Gelatin Types with Any Bloom Values

Modified Desolvation Method Enables Simple One-Step Synthesis of Gelatin Nanoparticles from Different Gelatin Types with Any Bloom Values

Modified desolvation method enables simple one-step synthesis of gelatin nanoparticles from different gelatin types with any bloom values

Highly Efficient Synthesis of Type B Gelatin and Low Molecular Weight Chitosan Nanoparticles: Potential Applications as Bioactive Molecule Carriers and Cell-Penetrating Agents

Contact Info

Product

Resources

About