Gelatinase activity in keloids and hypertrophic scars

Neely, Alice N.; Clendening, Chris E.; Gardner, Jason D.; Greenhalgh, David G.; Warden, Glenn D.

doi:10.1046/j.1524-475x.1999.00166.x

Cited by 83 publications

(67 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In our study, MMP-2 expression was consistent with the tissue repair process described by Neely et al [41], in which this MMP plays an active role in the constant remodelling of damaged tissue. In the HR and DRPGH groups, MMP-2 synthesis occurs early on, since at 3 days, the epidermis had completely closed and the dermis was of an organised structure.…”

Section: Discussionsupporting

confidence: 92%

A novel controlled drug-delivery system for growth hormone applied to healing skin wounds in diabetic rats

Gimeno

García-Esteo²,

García‐Honduvilla

et al. 2003

Journal of Biomaterials Science, Polymer Edition

View full text Add to dashboard Cite

Abstract-Controlled release systems for drugs, hormones and growth factors can be particularly useful in tissue repair processes. These systems act as a biodegradable support containing the substance to be delivered, allowing their gradual release. In the past years, the local application of growth factors has acquired special relevance as a therapeutic option for use in subjects who show de cient tissue scarring, the hormone dose being the limiting factor for its success. In this study, the in vitro biocompatibility of a copolymer formed by vinylpyrrolidone and 2-hydroxyethyl methacrylate, used as an administration vehicle for hGH, was evaluated. The system was then tested in vivo in terms of its capacity for healing incisional wounds in healthy and diabetic rats. For the in vitro studies, polymer and hormone degradation rates were determined, and polymer biocompatibility was evaluated in broblast cultures. In the in vivo experiments, an incision was made in the back of the animals, and polymers discs with/ without hGH, were introduced in the aperture. Morphological, immunohistochemical and morphometric evaluations were performed on wound tissue specimens 3-10 days after surgery. In vitro, the polymer was found to be biodegradable and showed no toxic effects on broblasts, the hormone being slowly released to the culture medium. In untreated diabetic rats, a delayed skin scarring and cell response were observed, compared to that noted in healthy animals. Skin closure, keratinisation and brosis occurred earlier in the presence of the polymer-hGH system. The use of this co-polymer as an administration vehicle for hGH improves the wound scarring process in the pathological setting of diabetes.

show abstract

Section: Discussionsupporting

confidence: 92%

A novel controlled drug-delivery system for growth hormone applied to healing skin wounds in diabetic rats

Gimeno

García-Esteo²,

García‐Honduvilla

et al. 2003

Journal of Biomaterials Science, Polymer Edition

View full text Add to dashboard Cite

show abstract

“…In keloids, besides overproduction of dermal collagen and other fibroblast proteins, also disturbed ECM degradation is observed [11,19,20]. The FAP-α, a member of the group II integral serine proteases, may play an important role in remodeling of ECM and the invasive properties of keloids.…”

Section: Suzawa Et Al Revealed That Tranilast Selective Inhibition Omentioning

confidence: 99%

“…Poza nadprodukcją kolagenu i innych białek w keloidach obserwuje się również zaburzenia rozkładu ECM [11,19,20]. Białko FAP-α, zaliczane do grupy II integralnych proteaz serynowych, może odgrywać istotną rolę w przebudowie ECM oraz wpływać na właściwości inwazyjne keloidów.…”

Section: Suzawa Et Al Revealed That Tranilast Selective Inhibition Ounclassified

The effect of tranilast on fibroblast activation protein α (FAP-α) expression in normal and keloid fibroblasts in vitro

Antończak¹,

Jurzak²,

Adamczyk³

et al. 2017

View full text Add to dashboard Cite

Introduction. Tranilast (N-(3',4'-demethoxycinnamoyl)-anthranilic acid)is an anti-allergic drug. Its mechanism of action is based on the inhibition of antigen-induced release of chemical mediators from mast cells and basophils. It also reveals antifibroproliferative activities. These properties of tranilast are used in the treatment of hypertrophic scars and keloids. Keloids are characterized by incorrect extracellular matrix components turnover. Fibroblasts derived from keloids reveal overproduction of collagen type I and decreased degradation of extracellular matrix in comparison with normal fibroblasts. Fibroblast activation protein α (FAP-α) may play an important role in remodeling of extracellular matrix and the invasive properties of keloids. Objective. In the present study, the effect of tranilast on expression of FAP-α gene and its protein was evaluated in normal human dermal fibroblasts and fibroblasts derived from keloids cultured in vitro. Materials and methods. In the first stage of the study, the influence of tranilast on cell viability was estimated. The second stage of the study included the quantitative evaluation of FAP-α mRNA expression in normal and keloid fibroblasts treated with tranilast. The third stage of the study comprised fibroblast activation protein α expression analysis in the examined cells treated with tranilast. Results and conclusions. The expression of FAP-α gene and fibroblast activation protein α is higher in keloid fibroblasts. Tranilast at concentrations of 3 μM and 30 μM up-regulated mRNA FAP-α expression in normal fibroblasts but did not influence keloid fibroblasts. The drug, at concentrations of 30 μM and 300 μM up-regulated fibroblast activation protein α expression in normal fibroblasts and did not influence keloid fibroblasts. Tranilast antiproliferative effect is not associated with FAP-α expression in keloid fibroblasts. Original article/Praca oryginalnaThe effect of tranilast on fibroblast activation protein α (FAP-α) expression in normal and keloid fibroblasts in vitro Wpływ tranilastu na ekspresję białka aktywującego fibroblasty α (FAP-α) w fibroblastach prawidłowych oraz fibroblastach keloidowych in vitro

show abstract

“…Previous reports exist on keloids and MMP. Neely et al reported that MMP-2 activity was significantly increased in hypertrophic scars and keloids (30). Oriente and co-workers reported that TGF-ß1 induces upregulation of MMPs in keloid fibroblasts (31).…”

Section: Discussionmentioning

confidence: 99%

TGF-β1 antisense therapy modulates expression of matrix metalloproteinases in keloid-derived fibroblasts

Sadick¹,

Herberger²,

Riedel³

et al. 2008

Int J Mol Med

View full text Add to dashboard Cite

Abstract. Transforming growth factor-ß1 (TGF-ß1) has been identified as an important regulator of wound healing. Recent developments in molecular therapy offer exciting prospects for the modulation of wound healing, specifically those targeting TGF-ß1. The purpose of this study was to analyze the effect of TGF-ß1 targeting on the expression of matrix metalloproteinases (MMPs) in fibroblasts cultured from earlobe keloids. The expression of MMP-2 and -9 in tissue samples from keloids was investigated by immunohistochemistry. The effect of TGF-ß1 targeting using antisense oligonucleotides on the expression of MMPs in keloid-derived fibroblasts was analysed by ELISA and multiplex RT-PCR. Immunohistochemical studies demonstrated an increased expression of MMP protein in tissue samples from keloids compared to normal human skin. Antisense TGF-ß1 oligonucleotide treatment significantly downregulated MMP-9 secretion in vitro. In conclusion, TGF-ß1 antisense oligonucleotide technology may be a potential therapeutic option for the inhibition of proteolytic tissue destruction in keloids.

show abstract

Gelatinase activity in keloids and hypertrophic scars

Cited by 83 publications

References 24 publications

A novel controlled drug-delivery system for growth hormone applied to healing skin wounds in diabetic rats

A novel controlled drug-delivery system for growth hormone applied to healing skin wounds in diabetic rats

The effect of tranilast on fibroblast activation protein α (FAP-α) expression in normal and keloid fibroblasts in vitro

TGF-β1 antisense therapy modulates expression of matrix metalloproteinases in keloid-derived fibroblasts

Contact Info

Product

Resources

About