Geminiviruses encode additional small proteins with specific subcellular localizations and virulence function

Gong, Pan; Tan, Hongjian; Zhao, Shuaiyang; Li, Hao; Liu, Hui; Ma, Yu; Zhang, Xi; Rong, Junjie; Fu, Xing; Lozano‐Durán, Rosa; Li, Fangfang; Zhou, Xueping

doi:10.1038/s41467-021-24617-4

Cited by 89 publications

(49 citation statements)

References 57 publications

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“…But NW bipartite has AV2 genes analogue to this V2 gene of monopartite ( Sudarshana et al, 1998 ; Rojas et al, 2005 ). V3 protein is also present, essential for complete viral infection and works as an RNA silencing suppressor ( Gong et al, 2021 ). DNA-B is found in bipartite genome.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: Emergence of Asian endemic begomoviruses as a pandemic threat

Qureshi

Lal²,

Nawaz-ul-Rehman³

et al. 2022

Front. Plant Sci.

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Plant viruses are responsible for the most devastating and commercially significant plant diseases, especially in tropical and subtropical regions. The genus begomovirus is the largest one in the family Geminiviridae, with a single-stranded DNA genome, either monopartite or bipartite. Begomoviruses are transmitted by insect vectors, such as Bemisia tabaci. Begomoviruses are the major causative agents of diseases in agriculture globally. Because of their diversity and mode of evolution, they are thought to be geographic specific. The emerging begomoviruses are of serious concern due to their increasing host range and geographical expansion. Several begomoviruses of Asiatic origin have been reported in Europe, causing massive economic losses; insect-borne transmission of viruses is a critical factor in virus outbreaks in new geographical regions. This review highlights crucial information regarding Asia’s four emerging and highly destructive begomoviruses. We also provided information regarding several less common but still potentially important pathogens of different crops. This information will aid possible direction of future studies in adopting preventive measures to combat these emerging viruses.

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Section: Introductionmentioning

confidence: 99%

RETRACTED: Emergence of Asian endemic begomoviruses as a pandemic threat

Qureshi

Lal²,

Nawaz-ul-Rehman³

et al. 2022

Front. Plant Sci.

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“…It is noteworthy that we applied the 10 kDa threshold in the prediction of SbYLCV-encoded proteins, as shown in previous studies. A recent study showed that geminiviruses also encode small additional ORFs (size range 5-10 kDa) with specific subcellular localization and functions [34]. Additional ORF was also identified in the viral sense of the betasatellite genome and was demonstrated to have a critical role in virus…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Pathogenicity of a Novel Soybean-Infecting Monopartite Geminivirus in China

Wang

Cheng

et al. 2022

Viruses

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Soybean is a major legume crop that plays an important role in food production, industrial production, and animal husbandry. Here, we characterize a novel soybean-infecting monopartite geminivirus identified in China. Analysis of the contigs de novo assembled from sequenced small interfering RNAs, followed by PCR, cloning, and sequencing, the complete viral genome was determined to be 2782 nucleotides. The genome contains the conserved nonanucleotide sequence, TAATATTAC and other sequence features typical of the family Geminiviridae, and encodes two and four open reading frames in the virion-sense and the complementary-sense strands, respectively. Genome-wide pairwise identity analysis revealed that the novel virus shares less than 65.6% identity with previously characterized geminiviruses. Phylogenetic and recombination analysis indicated that this virus was placed in a unique taxon within the family Geminiviridae and potentially arose from recombination. An infectious clone of this virus was further constructed and its infectivity was tested in different species of plants. Successful infection and characteristic symptoms were observed in Glycine max, Nicotiana benthamiana, N. tabacum, N. glutinosa, and N. tabacum cv. Samsun plants. Taken together, this virus represents a member of an unclassified genus of the family Geminiviridae, for which the name soybean yellow leaf curl virus is proposed.

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“…Intriguingly, the GLDV-2 isolate encodes an ORF putatively encoding AC6 in a complementary strand sense partially overlapped with the AV1 gene, which is syntenically conserved in some OW monopartite begomoviruses belonging to the sweepovirus clade. Recently, it has been described that additional hypothetical ORFs encoded by the Tomato yellow leaf curl virus (TYLCV) genome are expressed and play a role during viral infection, implying that the repertoire of geminiviral proteins can be expanded ( Gong et al, 2021 ). A phylogenetic analysis using representative members of the main begomovirus American clades for tree construction grouped both GLDV isolates in the clade of the monopartite NW begomovirus, Tomato leaf deformation virus (ToLDeV).…”

Section: Discussionmentioning

confidence: 99%

From Metagenomics to Discovery of New Viral Species: Galium Leaf Distortion Virus, a Monopartite Begomovirus Endemic in Mexico

et al. 2022

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Begomoviruses (Family Geminiviridae) are a major group of emerging plant viruses worldwide. The knowledge of begomoviruses is mostly restricted to crop plant systems. Nevertheless, it has been described that non-cultivated plants are important reservoirs and vessels of viral evolution that leads to the emergence of new diseases. High-throughput sequencing (HTS) has provided a powerful tool for speeding up the understanding of molecular ecology and epidemiology of plant virome and for discovery of new viral species. In this study, by performing earlier metagenomics library data mining, followed by geminivirus-related signature single plant searching and RCA-based full-length viral genome cloning, and based on phylogenetic analysis, genomes of two isolates of a novel monopartite begomovirus species tentatively named Galium leaf distortion virus (GLDV), which infects non-cultivated endemic plant Galium mexicanum, were identified in Colima, Mexico. Analysis of the genetic structure of both isolates (GLDV-1 and GLDV-2) revealed that the GLDV genome displays a DNA-A-like structure shared with the new world (NW) bipartite begomoviruses. Nonetheless, phylogenetic analysis using representative members of the main begomovirus American clades for tree construction grouped both GLDV isolates in a clade of the monopartite NW begomovirus, Tomato leaf deformation virus (ToLDeV). A comparative analysis of viral replication regulatory elements showed that the GLDV-1 isolate possesses an array and sequence conservation of iterons typical of NW begomovirus infecting the Solanaceae and Fabaceae families. Interestingly, GLDV-2 showed iteron sequences described only in monopartite begomovirus from OW belonging to a sweepovirus clade that infects plants of the Convolvulaceae family. In addition, the rep iteron related-domain (IRD) of both isolates display FRVQ or FRIS amino acid sequences corresponding to NW and sweepobegomovirus clades for GMV-1 and GMV-2, respectively. Finally, the lack of the GLDV DNA-B segment (tested by molecular detection and biological assays using GLDV-1/2 infectious clones) confirmed the monopartite nature of GLDV. This is the first time that a monopartite begomovirus is described in Mexican ecosystems, and “in silico” geometagenomics analysis indicates that it is restricted to a specific region. These data revealed additional complexity in monopartite begomovirus genetics and geographic distribution and highlighted the importance of metagenomic approaches in understanding global virome ecology and evolution.

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Geminiviruses encode additional small proteins with specific subcellular localizations and virulence function

Cited by 89 publications

References 57 publications

RETRACTED: Emergence of Asian endemic begomoviruses as a pandemic threat

RETRACTED: Emergence of Asian endemic begomoviruses as a pandemic threat

Molecular Characterization and Pathogenicity of a Novel Soybean-Infecting Monopartite Geminivirus in China

From Metagenomics to Discovery of New Viral Species: Galium Leaf Distortion Virus, a Monopartite Begomovirus Endemic in Mexico

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