Gender differences in biomechanical properties of intramural coronary resistance arteries of rats, an in vitro microarteriographic study

Mátrai, Máté; Mericli, Metin; Nádasy, György L.; Szekeres, Mária; Várbı́ró, Szabolcs; Bánhidy, Ferenc; Ács, Nándor; Monos, E.; Székács, Béla

doi:10.1016/j.jbiomech.2006.04.002

Cited by 10 publications

(9 citation statements)

References 29 publications

(46 reference statements)

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“…Nevertheless, in previous studies we found that the presence of estrogen upregulates eNOS and NO production and the dilator capacity of arterioles [45, 46]. Also, we previously found sex differences in functional and biomechanical characteristics of coronary arteries: in female coronaries constrictor responses were decreased, while estrogen replacement therapy after ovariectomy augmented bradykinin-induced NO-mediated dilation in these vessels [47, 48]. In brief, all these findings warrant future studies on how exercise programs affect the vascular function and morphology of female species.…”

Section: Discussionmentioning

confidence: 99%

Remodeling of Wall Mechanics and the Myogenic Mechanism of Rat Intramural Coronary Arterioles in Response to a Short-Term Daily Exercise Program: Role of Endothelial Factors

et al. 2018

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Purpose: Exercise elicits early adaptation of coronary vessels enabling the coronary circulation to respond adequately to higher flow demands. We hypothesized that short-term daily exercise induces biomechanical and functional remodeling of the coronary resistance arteries related to pressure. Methods: Male rats were subjected to a progressively increasing 4-week treadmill exercise program (over 60 min/day, 1 mph in the final step). In vitro pressure-diameter measurements were performed on coronary segments (119 ± 5 μm in diameter at 50 mm Hg) with microarteriography. The magnitude of the myogenic response and contribution of endogenous nitric oxide and prostanoid production to the wall mechanics and pressure-diameter relationship were assessed. Results: Arterioles isolated from exercised ani mals – compared to the sedentary group – had thicker walls, increased distensibility, and a decreased elastic modulus as a result of reduced wall stress in the low pressure range. The arterioles of exercised rats exhibited a more powerful myogenic response and less endogenous vasoconstrictor prostanoid modulation at higher pressures, while vasodilator nitric oxide modulation of diameter was augmented at low pressures (< 60 mm Hg). Conclusions: A short-term daily exercise program induces remodeling of rat intramural coronary arterioles, likely resulting in a greater range of coronary autoregulatory function (constrictor and dilator reserves) and more effective protection against great changes in intraluminal pressure, contributing thereby to the optimization of coronary blood flow during exercise.

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Section: Discussionmentioning

confidence: 99%

Remodeling of Wall Mechanics and the Myogenic Mechanism of Rat Intramural Coronary Arterioles in Response to a Short-Term Daily Exercise Program: Role of Endothelial Factors

et al. 2018

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“…Differences between male and female vascular function and disease risk are now well established. Some sexspecific characteristics of resistance artery function were previously published by our group [15,16]. Sex differences regarding several aspects of cardiovascular adaptation in response to physical exercise have previously been proven [17,18].…”

Section: Introductionmentioning

confidence: 89%

“…The chest was opened, the heart was removed, and the weight of the heart was measured. Thereafter, in a cold Krebs-Ringer solution, from an intramural branch of the left anterior descending coronary artery, resistance-sized arteries with an outer diameter of 200 μm [16] were prepared by careful microdissection under a preparation microscope (Wild, M3Z, Leica, Olympus Heerbrugg, Switzerland), as described previously [20]. The arteriolar segment with a length of approximately 2 mm was placed in a tissue bath with a glass bottom filled with a normal Krebs-Ringer solution (37°C).…”

Section: In Vitro Pressure Arteriography Of Intramural Coronary Arteriesmentioning

confidence: 99%

Long-term exercise results in morphological and biomechanical changes in coronary resistance arterioles in male and female rats

et al. 2020

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Background: Biomechanical remodeling of coronary resistance arteries in physiological left ventricular hypertrophy has not yet been analyzed, and the possible sex differences are unknown. Methods: Wistar rats were divided into four groups: male and female sedentary controls (MSe and FSe) and male and female animals undergoing a 12-week intensive swim training program (MEx and FEx). On the last day, the in vitro contractility, endothelium-dependent dilatation, and biomechanical properties of the intramural coronary resistance arteries were investigated by pressure microarteriography. Elastica and collagen remodeling were studied in histological sections. Results: A similar outer radius and reduced inner radius resulted in an elevated wall to lumen ratio in the MEx and FEx animals compared to that in the sedentary controls. The wall elastic moduli increased in the MEx and FEx rats. Spontaneous and TxA 2 agonist-induced tone was increased in the FEx animals, whereas endothelium-dependent relaxation became more effective in MEx rats. Arteries of FEx rats had stronger contraction, while arteries of MEx animals had improved dilation. Conclusions: According to our results, the coronary arterioles adapted to an elevated load during long-term exercise, and this adaptation depended on sex. It is important to emphasize that in addition to differences, we also found many similarities between the sexes in the adaptive response to exercise. The observed sport adaptation in the coronary resistance arteries of rats may contribute to a better understanding of the physiological and pathological function of these arteries in active and retired athletes of different sexes.

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“…An angiotensin-II-induced hypertension animal model resulted in pronounced vascular biomechanical, contractility, and pharmacon reactivity changes. Sex differences in the impairments of coronary arterioles have been found [ 12 , 13 ]. It is also well known that cardiovascular risk shows gender difference.…”

Section: Introductionmentioning

confidence: 99%

Vitamin D Deficiency Induces Elevated Oxidative and Biomechanical Damage in Coronary Arterioles in Male Rats

et al. 2020

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Background: Several reports prove interconnection between vitamin D (VD) deficiency and increased cardiovascular risk. Our aim was to investigate the effects of VD status on biomechanical and oxidative–nitrative (O–N) stress parameters of coronary arterioles in rats. Methods: 4-week-old male Wistar rats were divided into a control group (11 animals) with optimal VD supply (300 IU/kgbw/day) and a VD-deficient group (11 animals, <5 IU/kg/day). After 8 weeks, coronary arteriole segments were prepared. Geometrical, elastic, and biomechanical characteristics were measured by in vitro arteriography. O–N stress markers were investigated by immunohistochemistry. Results: Inner radius decreased; wall thickness and wall-thickness/lumen diameter ratio increased; tangential wall stress and elastic modulus were reduced in VD-deficient group. No difference could be found in wall-cross-sectional area, intima-media area %. While the elastic elements of the vessel wall decreased, the α-smooth muscle actin (α-SMA) immunostaining intensity showed no changes. Significant elevation was found in the lipid peroxidation marker of 4-hidroxy-2-nonenal (HNE), while other O–N stress markers staining intensity (poly(ADP)ribose, 3-nitrotyrosine) did not change. Conclusions: Inward eutrophic remodeling has developed. The potential background of these impairments may involve the initial change in oxidative damage markers (HNE). These mechanisms can contribute to the increased incidence of the cardiovascular diseases in VD deficiency.

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Gender differences in biomechanical properties of intramural coronary resistance arteries of rats, an in vitro microarteriographic study

Cited by 10 publications

References 29 publications

Remodeling of Wall Mechanics and the Myogenic Mechanism of Rat Intramural Coronary Arterioles in Response to a Short-Term Daily Exercise Program: Role of Endothelial Factors

Remodeling of Wall Mechanics and the Myogenic Mechanism of Rat Intramural Coronary Arterioles in Response to a Short-Term Daily Exercise Program: Role of Endothelial Factors

Long-term exercise results in morphological and biomechanical changes in coronary resistance arterioles in male and female rats

Vitamin D Deficiency Induces Elevated Oxidative and Biomechanical Damage in Coronary Arterioles in Male Rats

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