Gender Differences in Ca
            <sup>2+</sup>
            Entry Mechanisms of Vasoconstriction in Wistar-Kyoto and Spontaneously Hypertensive Rats

Crews, Janice K.; Murphy, Jason G.; Khalil, Raouf A.

doi:10.1161/01.hyp.34.4.931

Cited by 43 publications

(62 citation statements)

References 31 publications

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“…Consistent with results reported by others in rat aorta [15][16][17], we demonstrated a higher maximal relaxation to acetylcholine, and a lower contractile response to phenylephrine and less sensitivity to angiotensin II in aortae from females compared to males. Estrogen deficiency was shown to augment contractions to angiotensin II, with AT 1 receptor overexpression, and phenylephrine, which is reversed by estrogen substitution therapy, in isolated aortic rings from female rats [16,17]. Administration of estrogen for 7 days to male and female rats was demonstrated to increase endothelial relaxation or suppress the contractions most likely by increasing production of endothelial nitric oxide [22][23][24].…”

Section: Discussionsupporting

confidence: 93%

“…Moreover, the female sex hormone estrogen seems to be involved in the regulation of vascular tension because estrogen, at physiological concentrations, causes relaxation by activating nitric oxide synthesis, which may lead to gender-related differences [21]. Consistent with results reported by others in rat aorta [15][16][17], we demonstrated a higher maximal relaxation to acetylcholine, and a lower contractile response to phenylephrine and less sensitivity to angiotensin II in aortae from females compared to males. Estrogen deficiency was shown to augment contractions to angiotensin II, with AT 1 receptor overexpression, and phenylephrine, which is reversed by estrogen substitution therapy, in isolated aortic rings from female rats [16,17].…”

Section: Discussionsupporting

confidence: 91%

“…In the present study, we assessed whether relaxation to acetylcholine and vasoconstrictions to phenylephrine and angiotensin II are changed in aortae from resveratrol-treated healthy rats, whether aortic superoxide production capacity in response to provocation by angiotensin II and NAD(P)H is reduced, and whether the effect of resveratrol is modified by gender and by the presence or absence of endothelium. It is well known that endothelium modulates vascular contractility, and its function may change between males and females [15][16][17]. Increasing evidence demonstrated that endothelial factor nitric oxide promotes vascular health against superoxide, angiotensin II and catecholamine-induced insults [18].…”

Section: Introductionmentioning

confidence: 99%

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Resveratrol Supplementation Gender Independently Improves Endothelial Reactivity and Suppresses Superoxide Production in Healthy Rats

Söylemez

Sepici

Akar

2009

Cardiovasc Drugs Ther

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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Resveratrol Supplementation Gender Independently Improves Endothelial Reactivity and Suppresses Superoxide Production in Healthy Rats

Söylemez

Sepici

Akar

2009

Cardiovasc Drugs Ther

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“…In comparison, ER-mediated relaxation in the carotid, mesenteric, and renal artery appears to mainly involve an endothelium-independent mechanism in VSM because 1) E 2 -, PPT-, DPN-, and G1-induced relaxation of the carotid, mesenteric, and renal artery was not blocked by L-NAME, indomethacin, TEA, or endothelium removal; 2) ER agonist-induced relaxation was still greater in the mesenteric and renal artery and in the carotid artery than in the aorta even after treatment with blockers of endothelium-derived vasodilators or endothelium removal; and 3) immunohistochemistry showed ER␣, ER␤, and GPER in the tunica media and VSM of the aorta, carotid artery, mesenteric artery, and renal artery of pregnant rats. These data are consistent with reports that E 2 causes relaxation of the endothelium-denuded aorta and coronary artery (13,14,31), and inhibits Phe-and PGF 2␣ -induced contraction in VSM cells from the rat aorta and porcine coronary artery (60,61).…”

Section: Pregnancy-and E 2 /Er-related Adaptations In Vascular Functionsupporting

confidence: 92%

Adaptive increases in expression and vasodilator activity of estrogen receptor subtypes in a blood vessel-specific pattern during pregnancy

Mata

Reslan

et al. 2015

American Journal of Physiology-Heart and Circulatory Physiology

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RA. Adaptive increases in expression and vasodilator activity of estrogen receptor subtypes in a blood vessel-specific pattern during pregnancy. Am J Physiol Heart Circ Physiol 309: H1679 -H1696, 2015. First published September 25, 2015 doi:10.1152/ajpheart.00532.2015.-Normal pregnancy is associated with adaptive hemodynamic, hormonal, and vascular changes, and estrogen (E 2) may promote vasodilation during pregnancy; however, the specific E 2 receptor (ER) subtype, post-ER signaling mechanism, and vascular bed involved are unclear. We tested whether pregnancy-associated vascular adaptations involve changes in the expression/ distribution/activity of distinct ER subtypes in a blood vessel-specific manner. Blood pressure (BP) and plasma E 2 were measured in virgin and pregnant (day 19) rats, and the thoracic aorta, carotid artery, mesenteric artery, and renal artery were isolated for measurements of ER␣, ER␤, and G protein-coupled receptor 30 [G protein-coupled ER (GPER)] expression and tissue distribution in parallel with relaxation responses to E 2 (all ERs) and the specific ER agonist 4,4=,4Љ-(4-propyl-[1H]-pyrazole-1,3,5-triyl)-tris-phenol (PPT; ER␣), diarylpropionitrile (DPN; ER␤), and G1 (GPER). BP was slightly lower and plasma E 2 was higher in pregnant versus virgin rats. Western blots revealed increased ER␣ and ER␤ in the aorta and mesenteric artery and GPER in the aorta of pregnant versus virgin rats. Immunohistochemistry revealed that the increases in ERs were mainly in the intima and media. In phenylephrineprecontracted vessels, E 2 and PPT caused relaxation that was greater in the aorta and mesenteric artery but similar in the carotid and renal artery of pregnant versus virgin rats. DPN-and G1-induced relaxation was greater in the mesenteric and renal artery than in the aorta and carotid artery, and aortic relaxation to G1 was greater in pregnant versus virgin rats. The nitric oxide synthase inhibitor N -nitro-L-arginine methyl ester with or without the cyclooxygenase inhibitor indomethacin with or without the EDHF blocker tetraethylammonium or endothelium removal reduced E 2, PPT, and G1-induced relaxation in the aorta of pregnant rats, suggesting an endothelium-dependent mechanism, but did not affect E 2-, PPT-, DPN-, or G1-induced relaxation in other vessels, suggesting endothelium-independent mechanisms. E 2, PPT, DPN, and G1 caused relaxation of Ca 2ϩ entry-dependent KCl contraction, and the effect of PPT was greater in the mesenteric artery of pregnant versus virgin rats. Thus, during pregnancy, an increase in ER␣ expression in endothelial and vascular smooth muscle layers of the aorta and mesenteric artery is associated with increased ER␣-mediated relaxation via endothelium-derived vasodilators and inhibition of Ca 2ϩ entry into vascular smooth muscle, supporting a role of aortic and mesenteric arterial ER␣ in pregnancyassociated vasodilation. GPER may contribute to aortic relaxation while enhanced ER␤ expression could mediate other genomic vascular effects during pregnancy.

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“…Membrane depolarization by high KCl mainly stimulates Ca 2+ entry from the extracellular space (28). Reports that KCl-induced smooth muscle contraction, Ca 2+ infl ux and [Ca 2+ ] i are greater in males than females further support possible gender differences in the Ca 2+ -entry mechanisms (29,30). These results support our observation.…”

Section: Discussionmentioning

confidence: 95%

Responses of acrylamide-treated rat bladders

Nurullahoglu-Atalik¹,

Okudan²,

Belviranlı³

et al. 2012

BLL

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Abstract:Objective: Acrylamide (ACR) is a chemical used in many industries around the world and more recently was found to be formed naturally in foods cooked at high temperatures. ACR was shown to be a neurotoxicant, reproductive toxicant, and carcinogen in animal species. The aim of the present study is to evaluate the infl uence of ACR treatment on urinary bladder responses to carbachol (10 -9 -3x10 -4 M) and potassium chloride (KCl; 5-100 mM), each of them causes receptor-dependent and receptor-independent contractions, respectively. We also examined the role of gender in these responses. Material and methods: Rats of both genders were divided into three groups as follows: (1) Control animals (2) ACR-I; ACR-treated (2 mg/kg-d for 90 days) (3) ACR-II; ACR-treated (5 mg/kg-d for 90 days). Results: In rats treated with ACR, the EC 50 values of carbachol and KCl, but not the maximal response, to both agents were signifi cantly higher than in control group. Histopathological parameters such as edema, congestion, infl ammatory cells, microvascular proliferation, fi brosis, eosinophils, mast cells and epithelial damage were all higher in the ACR-treated group than in the controls.Conclusions: These results demonstrate for the fi rst time that ACR-treatment can induce urinary bladder injury (Tab. 4, Fig. 4, Ref. 30). Full Text in PDF www.elis.sk.

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Gender Differences in Ca ²⁺ Entry Mechanisms of Vasoconstriction in Wistar-Kyoto and Spontaneously Hypertensive Rats

Cited by 43 publications

References 31 publications

Resveratrol Supplementation Gender Independently Improves Endothelial Reactivity and Suppresses Superoxide Production in Healthy Rats

Resveratrol Supplementation Gender Independently Improves Endothelial Reactivity and Suppresses Superoxide Production in Healthy Rats

Adaptive increases in expression and vasodilator activity of estrogen receptor subtypes in a blood vessel-specific pattern during pregnancy

Responses of acrylamide-treated rat bladders

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Gender Differences in Ca 2+ Entry Mechanisms of Vasoconstriction in Wistar-Kyoto and Spontaneously Hypertensive Rats

Cited by 43 publications

References 31 publications

Resveratrol Supplementation Gender Independently Improves Endothelial Reactivity and Suppresses Superoxide Production in Healthy Rats

Resveratrol Supplementation Gender Independently Improves Endothelial Reactivity and Suppresses Superoxide Production in Healthy Rats

Adaptive increases in expression and vasodilator activity of estrogen receptor subtypes in a blood vessel-specific pattern during pregnancy

Responses of acrylamide-treated rat bladders

Contact Info

Product

Resources

About

Gender Differences in Ca ²⁺ Entry Mechanisms of Vasoconstriction in Wistar-Kyoto and Spontaneously Hypertensive Rats