Gender-related differences in morphology and thermogenic capacity of brown adipose tissue mitochondrial subpopulations

Justo, Roberto; Frontera, Margalida; Pujol, Esperanza; Rodríguez‐Cuenca, Sergio; Lladó, Isabel; García-Palmer, Francisco J.; Roca, Pilar; Gianotti, Magdalena

doi:10.1016/j.lfs.2004.08.019

Cited by 67 publications

(50 citation statements)

References 37 publications

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“…These findings are in agreement with previous works showing greater recruitment in BAT of female rats [21,33]. In particular, it has been shown that ad libitum female rats have larger mitochondria, with longer cristae and higher cristae density [21].…”

Section: Discussionsupporting

confidence: 93%

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Sex Differences in Brown Adipose Tissue Thermogenic Features During Caloric Restriction

Valle

García-Palmer

Oliver

et al. 2007

Cell Physiol Biochem

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Caloric restriction (CR) studies have shown that females rats conserve energy more efficiently, showing a higher resistance to weight loss and higher protection of vital organs mass than male rats. Gender-dependent inactivation of thermogenesis in brown adipose tissue (BAT) has been proposed as one of these possible energy conserving mechanisms. To study the changes underlying this inactivation in rats, a three month study with 40% CR was undertaken to unravel the effects on BAT recruitment. Under ad libitum conditions female rats had greater BAT recruitment and greater oxygen consumption than their male counterparts. Total and mitochondrial protein, as well as triglyceride and DNA content were more reduced in restricted female rats than in restricted males. Similarly, the levels of key BAT functional proteins (UCP1, LPL, HSL, TFAM) were more reduced in restricted females, whereas no changes were found in mitochondrial DNA levels (mtDNA) and OXPHOS activities in males and females. Furthermore, α _2A/β ₃ adrenergic receptor ratio remained constant in male rats whereas in female rats CR increased 60%. In conclusion, our results suggest that female rats, whose BAT thermogenic activity is higher in ad libitum conditions, is depressed during CR. This inactivation involves the mitochondrial differentiation process and lipolytic system and could be due, at least in part, to the unfavourable adrenergic receptor balance for thermogenic activation.

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Section: Discussionsupporting

confidence: 93%

“…The ad libitum fed female rats showed the lowest ratio mtDNA/mtprotein, indicating a tendency for a higher degree of maturation, which was in agreement with previous reports [21,33]. On the contrary, restricted female rats showed similar mtDNA/mtprotein ratio than males (which levels were not affected by CR).…”

Section: Mitochondrial Dna and Tfam Levelssupporting

confidence: 92%

Sex Differences in Brown Adipose Tissue Thermogenic Features During Caloric Restriction

Valle

García-Palmer

Oliver

et al. 2007

Cell Physiol Biochem

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“…In line with the results of other studies, female gender, younger age, low/normal BMI and BF and autumn/winter seasonal pattern were more prevalent in BAT bearing patients (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35). However, the level of BAT metabolic activity demonstrated significant correlation only with age and the recent history of treatment.…”

Section: Discussionsupporting

confidence: 87%

Brown Adipose Tissue at F-18 FDG PET/CT: Correlation of Metabolic Parameter with Demographics and Cancer-Related Characteristics in Cancer Patients

et al. 2017

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Background: The effect of various environmental and intrinsic stimulators on the development of brown adipose tissue (BAT) has been widely investigated by PET-based researches. However, evidence regarding the influencing factors on the level of BAT metabolic activity is scarce. Objectives: The aim of the present study was to evaluate the frequency of cancer-related characteristics in addition to anthropometrics and demographics in BAT-bearing cancer patients at 18F-fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) scan and any correlation between the level of BAT metabolic activity and the influencing factors. Methods: Reports from a total of 3762 F-18 FDG PET/CT scans were retrospectively reviewed to identify BAT-bearing cancer population. Demographic, anthropometric and cancer related characteristics were recorded. Maximum standardized uptake values (SUVmax) was measured separately for each anatomical region. Descriptive quantitative variables were expressed as either frequency or mean. Independent T test, Mann-Withney U test, Pearson correlation coefficients, one-way analysis of variance and linear regression test (IBM SPSS version 23) were used as appropriate (P value <0.05) Results: Sixty-two F-18 FDG PET/CT studies demonstrated BAT related 18-F FDG uptake (1.6%, 32% male, 68% female, P = 0.007, mean age 22.9). Lymphoma (43.5%) and treatment response evaluation (54.54%) were the most frequent type of cancer and reason for referral, respectively. Most patients were in status partial or complete metabolic response to treatment. Fifty-four point eight percent of patients had at least one metabolically active cancer-related lesion. BAT detection rate was higher in females in all adult age groups, younger age (< 40 years old), body mass index (BMI) and body fat (BF) below the obesity cut off and autumn/winter seasons. The dominant distribution pattern of BAT depots was neck, mediastinum, paravertebral (33.87%) with the highest level of metabolic activity in the axillary region. SUVmax demonstrated a weak inverse correlation with age (0.015), evidence of active malignant disease and no recent treatment. Linear regression test demonstrated that age (P = 0.021) and recent treatment (P = 0.033) have independent correlation with BAT SUVmax. Conclusions: The present study provided evidence for age and chemotherapeutic agents on the level of BAT metabolism. In addition, there is a suggestion for different pathways involved in BAT development and regulation of the level of metabolic activity.

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“…Valle et al (2005) have found that Wistar female liver mitochondria consume more oxygen than male mitochondria. In fact, it seems that female mitochondria show a greater tendency to respiratory chain uncoupling (Justo et al, 2005). Recently, it has been proposed that increased uncoupling can increase survival (Brand et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of sex differences on mitochondrial bioenergetics and apoptosis in mice

Sanz

Hiona

Kujoth

et al. 2007

Experimental Gerontology

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SummaryIt has been postulated that the differences in longevity observed between organisms of different sexes within a species can be attributed to differences in oxidative stress. It is generally accepted that differences are due to the higher female estrogen levels. However, in some species males live the same or longer despite their lower estrogen values. Therefore, in the present study, we analyze key parameters of mitochondrial bioenergetics, oxidative stress and apoptosis in the B6 (C57Bl/6J) mouse strain. There are no differences between males and females in this mouse strain, although estrogen levels are higher in females. We did not find any differences in heart, skeletal muscle and liver mitochondrial oxygen consumption (State 3 and State 4) and ATP content between male and female mice. Moreover, mitochondrial H 2 O 2 generation and oxidative stress levels determined by cytosolic protein carbonyls and concentration of 8-hydroxy-2′-deoxyguanosine in mitochondrial DNA were similar in both sexes. In addition, markers of apoptosis (caspase-3, caspase-9 and mono-and oligonucleosomes: the apoptosis index) were not different between male and female mice. These data show that there are no differences in mitochondrial bioenergetics, oxidative stress and apoptosis due to gender in this mouse strain according with the lack of differences in longevity. These results support the Mitochondrial Free Radical Theory of Ageing, and indicate that oxidative stress generation independent of estrogen levels determines ageing rate.

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Gender-related differences in morphology and thermogenic capacity of brown adipose tissue mitochondrial subpopulations

Cited by 67 publications

References 37 publications

Sex Differences in Brown Adipose Tissue Thermogenic Features During Caloric Restriction

Sex Differences in Brown Adipose Tissue Thermogenic Features During Caloric Restriction

Brown Adipose Tissue at F-18 FDG PET/CT: Correlation of Metabolic Parameter with Demographics and Cancer-Related Characteristics in Cancer Patients

Evaluation of sex differences on mitochondrial bioenergetics and apoptosis in mice

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