Gene action in the resistance of peppers (Capsicum annuum) to Phytophthora stem blight (Phytophthora capsici L.)

Bartual, R.; Carbonell, E. A.; Marsal, José Ignacio; Tello, Julio C.; Campos, Thayllane de

doi:10.1007/bf00039608

Cited by 23 publications

(6 citation statements)

References 18 publications

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“…Numerous sources of resistance against P. capsici have been identified in the cultivated species C. annuum, including Criollo de Morelos 334 (CM334), PI201232, PI201234 and AC2258, among which CM334 shows consistently high levels of resistance (Bartual et al 1991;Oelke et al 2003). Phytophthora resistance is often characterized as polygenic or quantitative, although simple inheritance models have been proposed.…”

Section: Introductionmentioning

confidence: 99%

Combined use of bulked segregant analysis and microarrays reveals SNP markers pinpointing a major QTL for resistance to Phytophthora capsici in pepper

et al. 2014

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Bulked segregant analysis (BSA) using Affymetrix GeneChips revealed candidate genes underlying the major QTL for Phytophthora capsici resistance in Capsicum. Using the candidate genes, reliable markers for Phytophthora resistance were developed and validated. Phytophthora capsici L. is one of the most destructive pathogens of pepper (Capsicum spp.). Resistance of pepper against P. capsici is controlled by quantitative trait loci (QTL), including a major QTL on chromosome 5 that is the predominant contributor to resistance. Here, to maximize the effect of this QTL and study its underlying genes, an F2 population and recombinant inbred lines were inoculated with P. capsici strain JHAI1-7 zoospores at a low concentration (3 × 10(3)/mL). Resistance phenotype segregation ratios for the populations fit a 3:1 and 1:1 (resistant:susceptible) segregation model, respectively, consistent with a single dominant gene model. Bulked segregant analysis (BSA) using Affymetrix GeneChips revealed a single position polymorphism (SPP) marker mapping to the major QTL. When this SPP marker (Phyto5SAR) together with other SNP markers located on chromosome 5 was used to confirm the position of the major QTL, Phyto5SAR showed the highest LOD value at the QTL. A scaffold sequence (scaffold194) containing Phyto5SAR was identified from the C. annuum genome database. The scaffold contained two putative NBS-LRR genes and one SAR 8.2A gene as candidates for contributing to P. capsici resistance. Markers linked to these genes were developed and validated by testing 100 F1 commercial cultivars. Among the markers, Phyto5NBS1 showed about 90% accuracy in predicting resistance phenotypes to a low-virulence P. capsici isolate. These results suggest that Phyto5NBS1 is a reliable marker for P. capsici resistance and can be used for identification of a gene(s) underlying the major QTL on chromosome 5.

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Section: Introductionmentioning

confidence: 99%

Combined use of bulked segregant analysis and microarrays reveals SNP markers pinpointing a major QTL for resistance to Phytophthora capsici in pepper

et al. 2014

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“…The moderately resistance genetic resources considered in this report can be used in breeding programs aimed at promoting P. capsici resistance via hybridization with other resistant lines or through recurrent selection, as has been demonstrated and recommended previously (Bartual et al, 1991(Bartual et al, , 1994Palloix et al, 1990). Muhanjilju, a commercial hybrid cultivar, exhibited a reasonable level of resistance in this study.…”

Section: Resultsmentioning

confidence: 68%

“…Tantan, a commercial rootstock cultivar, survived infection from both strains of P. capsici without presenting any above-ground symptoms but suffered a considerable degree of root rot. KC00256, originally known as P51 and a European breed that has been studied previously (Barksdale et al, 1984;Bartual et al, 1991Bartual et al, , 1994, remained symptomless above ground after Pc003 inoculation, but Pc005 induced severe heavy stem and root rot in many plants. KC00358-1 was the next most resistant line, exhibiting relatively consistent reactions to both pathogen strains.…”

Section: Resultsmentioning

confidence: 99%

Restorer Genotype for Male Sterile Cytoplasm of Genetic Resources Moderately Resistant to Phytophthora capsici in Capsicum Pepper

Kim¹,

Ahn²,

Lee³

et al. 2012

Korean Journal of Horticultural Science and Technology

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KC00256, KC00406, KC00462, KC00463, KC00820, and KC00821, the genetic resources that have previously been reported as moderately resistant to Phytophthora capsici, as well as the line KC01322, a new source of moderate resistance introduced from Laos, were tested against two strains (Pc003 and Pc005) of P. capsici. We also determined the nuclear restorer genotypes of these lines, in regards to their interaction with cytoplasmic male sterility, through crossing the resources with cytoplasmic male sterile Punggok-A (Srfrf) and determining the fertility of the F 1 hybrids. The studied lines exhibited a low level of resistance to both the strains of P. capsici compared to highly resistant CM334, but their response was fairly consistent for both P. capsici strains. KC00406, KC00462, KC00463, and KC01322 produced stable, male fertile F 1 plants indicating that they are restorers with genotype N(S)RfRf. KC00821 produced male sterile F 1 plants and was identified as a maintainer with genotype Nrfrf. The F 1 plants of the KC00820 cross, however, set a few male fertile flowers in the greenhouse at seedling stage, then became male sterile after being transplanted to the plastic greenhouse soil in May and remained so to the end of the growing season. Therefore, KC00820 is an unstable maintainer with genotype Nrfrf. The moderate resistance exhibited by these genetic resources may be integrated into breeding programs aimed at promoting higher levels resistance via recurrent selection or hybridization.

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“…Transgressive lines for resistance were obtained. Bartual et al (1991) studied inheritance for resistance to P. capsici, and epistasis was the main source of variation. Those authors concluded that recurrent selection is the most appropriate method for the transfer of resistance to a desired genotype.…”

Section: Breeding Methods and Programs Aimed At Anthracnose Resistancementioning

confidence: 99%

The state-of-the-art in the genetics of resistance to the colletotrichum species complex in Capsicum

Araújo,

Sudré,

Alencar

et al. 2023

Revisão Anual De Patologia De Plantas

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Anthracnose, caused by a complex of Colletotrichum species, causes severe damage in various crops, including sweet and chili peppers (Capsicum spp.). The present work is an overview of the breeding advances, challenges and limitations to the identification and characterization of genetic resistance and analytical tools employed for the development of Capsicum cultivars in breeding programs aimed at anthracnose resistance. The complexity and variability of the Capsicum-Colletotrichum pathosystem have practical implications for the germplasm resistance assessment protocols and the selection of representative populations of the pathogens. The methods and tools used for phenotypic evaluation must be adjusted to increase the reliability of the data, which in turn, is essential for the identification of unique genotypes able to contribute to the development of cultivars with durable resistance.

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Gene action in the resistance of peppers (Capsicum annuum) to Phytophthora stem blight (Phytophthora capsici L.)

Cited by 23 publications

References 18 publications

Combined use of bulked segregant analysis and microarrays reveals SNP markers pinpointing a major QTL for resistance to Phytophthora capsici in pepper

Combined use of bulked segregant analysis and microarrays reveals SNP markers pinpointing a major QTL for resistance to Phytophthora capsici in pepper

Restorer Genotype for Male Sterile Cytoplasm of Genetic Resources Moderately Resistant to Phytophthora capsici in Capsicum Pepper

The state-of-the-art in the genetics of resistance to the colletotrichum species complex in Capsicum

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