Gene characterization and expression pattern of Mx and Viperin genes in Dabry’s sturgeon<i>Acipenser dabryanus</i>

Zhang, Shuhuan; Lv, Xiaoyan; Deng, Dan; Zeng, Ling; Huang, Wei; Tang, Dongdong; Zhang, Lifeng; Xu, Qiaoqing; Wei, Qiwei

doi:10.1111/jai.13876

Cited by 7 publications

(4 citation statements)

References 53 publications

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“…In other fishes, the Mx gene is also ubiquitous in different tissues, such as sea bream [25] and Japanese flounder [6]. In the present study, we found that AdMx was expressed ubiquitously in all eight examined tissues, which is similar to the expression pattern in Cirrhinus mrigala [26] and Acipenser dabryanus [27]. Moreover, AdMx showed a relative high transcript level in the brain, kidney and spleen.…”

Section: Discussionsupporting

confidence: 79%

Characterization, Expression Pattern and Antiviral Activities of Mx Gene in Chinese Giant Salamander, Andrias davidianus

Liu

Zhou

et al. 2020

IJMS

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Mx, Myxovirus resistance is an important interferon-stimulated protein that mediates antiviral responses. In this study, the expression and activities of Chinese giant salamander, Andrias davidianus Mx gene, AdMx, were investigated. The AdMx cDNA sequence contains an open reading frame (ORF) of 2112 nucleotides, encoding a putative protein of 703 aa. Meanwhile, AdMx possesses the conserved tripartite GTP binding motif and a dynamin family signature. qRT-PCR analysis revealed a broad expression of AdMx in vivo, with the highest expression levels in brain, kidney and spleen. The AdMx expression level in kidney, spleen and muscle significantly increased at 6 h after Chinese giant salamander iridovirus (GSIV) infection and peaked at 48 h, while that in muscle cell line (GSM) was not noticeably up-regulated until 72 h post infection. Additionally, a plasmid expressing AdMx was constructed and transfected into the Chinese giant salamander GSM cells. The virus load and gene copies in AdMx over-expressed cells were significantly reduced compared with those in the control cells. Moreover, compared to the control cells, a lower level of virus major capsid protein (MCP) synthesis in AdMx over-expressed cells was confirmed by Western blot. These results collectively suggest that Mx plays an important antiviral role in the immune responses against GSIV in Chinese giant salamander.

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Section: Discussionsupporting

confidence: 79%

Characterization, Expression Pattern and Antiviral Activities of Mx Gene in Chinese Giant Salamander, Andrias davidianus

Liu

Zhou

et al. 2020

IJMS

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“…Another way relies on the catalytic activity of Viperin to generate 3′-deoxy-3′,4′-didehydro-CTP (ddhCTP), which can directly interfere with RNA synthesis and the replication of flaviviruses (33). Similar to mammal, fish viperin is considered an important antiviral gene because its expression is also induced by LPS, poly(I:C), or viruses (15,(34)(35)(36)(37)(38)(39)(40). However, little is known about the molecular mechanism underlying the antiviral effect of fish Viperin.…”

Section: Introductionmentioning

confidence: 99%

Divergent Antiviral Mechanisms of Two Viperin Homeologs in a Recurrent Polyploid Fish

Mou

et al. 2021

Front. Immunol.

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Polyploidy and subsequent diploidization provide genomic opportunities for evolutionary innovations and adaptation. The researches on duplicated gene evolutionary fates in recurrent polyploids have seriously lagged behind that in paleopolyploids with diploidized genomes. Moreover, the antiviral mechanisms of Viperin remain largely unclear in fish. Here, we elaborate the distinct antiviral mechanisms of two viperin homeologs (Cgviperin-A and Cgviperin-B) in auto-allo-hexaploid gibel carp (Carassius gibelio). First, Cgviperin-A and Cgviperin-B showed differential and biased expression patterns in gibel carp adult tissues. Subsequently, using co-immunoprecipitation (Co-IP) screening analysis, both CgViperin-A and CgViperin-B were found to interact with crucian carp (C. auratus) herpesvirus (CaHV) open reading frame 46 right (ORF46R) protein, a negative herpesvirus regulator of host interferon (IFN) production, and to promote the proteasomal degradation of ORF46R via decreasing K63-linked ubiquitination. Additionally, CgViperin-B also mediated ORF46R degradation through autophagosome pathway, which was absent in CgViperin-A. Moreover, we found that the N-terminal α-helix domain was necessary for the localization of CgViperin-A and CgViperin-B at the endoplasmic reticulum (ER), and the C-terminal domain of CgViperin-A and CgViperin-B was indispensable for the interaction with degradation of ORF46R. Therefore, the current findings clarify the divergent antiviral mechanisms of the duplicated viperin homeologs in a recurrent polyploid fish, which will shed light on the evolution of teleost duplicated genes.

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“…Immune‐related proteins such as IFNs, IFN receptors, interleukin 16/17, trim, lysozymes, antimicrobial peptides, and Toll‐like receptors (TLRs) have been characterised (Han et al., 2018; Kai et al., 2019; Youshen Li et al., 2019; Luo et al., 2018; Xu et al., 2019; Zhang, Xu, Boscari, et al., 2018; Zhang, Xu, Du, et al., 2018). The interferon‐stimulating, Mx and Viperin genes have also been reported in A. dabryanus (Zhang et al., 2019). However, there has been no report of PKR genes in A. dabryanus .…”

Section: Introductionmentioning

confidence: 89%

Molecular characterization and expression analysis of double‐stranded RNA‐dependent protein kinase (PKR) in Dabry’s sturgeon ( Acipenser dabryanus )

Zhang

Chen²,

Yuan

et al. 2021

J Appl Ichthyol

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Protein kinase RNA‐activated (PKR) is an important component of the innate immune system induced by interferon. This antiviral protein senses a variety of stresses and pathogen‐driven double‐stranded RNAs (dsRNAs). In the present study, we cloned and characterized the PKR gene from Dabry's sturgeon (Acipenser dabryanus). Bioinformatics analysis revealed that the AdPKR gene comprises a coding sequence (CDS) of 1848 bp, encoding a 616 amino acids (aa) polypeptide. AdPKR shares high aa identity/similarity with proteins from other fish, ranging from 33.4%/51.8% to 87.6%/91.2%, and relatively low aa identity (13.9%) and similarity (26.8%) with human PKR. The AdPKR polypeptide has two dsRNA‐binding motifs at the N‐terminus and a conserved catalytic kinase domain at the C‐terminus. The sturgeon PKR gene contains 17 exons split by 16 introns. Phylogenetic analysis clustered AdPKR together with its counterparts from other teleost fishes. Real‐time fluorescene quantitative PCR (RT‐qPCR) transcription profiles revealed highest AdPKR expression levels in brain, followed by middle kidney, skin, muscle and head kidney. AdPKR mRNA transcripts were significantly increased at 6 hr post‐stimulation (hps) with polyinosinic:polycytidylic acid (poly I:C), remained high at 24 hps, then decreased to normal levels at 48 hps. These results indicate that AdPKR might play a vital role in antiviral immune responses.

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Gene characterization and expression pattern of Mx and Viperin genes in Dabry’s sturgeonAcipenser dabryanus

Cited by 7 publications

References 53 publications

Characterization, Expression Pattern and Antiviral Activities of Mx Gene in Chinese Giant Salamander, Andrias davidianus

Characterization, Expression Pattern and Antiviral Activities of Mx Gene in Chinese Giant Salamander, Andrias davidianus

Divergent Antiviral Mechanisms of Two Viperin Homeologs in a Recurrent Polyploid Fish

Molecular characterization and expression analysis of double‐stranded RNA‐dependent protein kinase (PKR) in Dabry’s sturgeon ( Acipenser dabryanus )

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