Gene Cloning of an Alcohol Dehydrogenase from Thermophilic Alkane-Degrading Bacillus thermoleovorans B23.

KATO, TOMOHISA; Miyanaga, A.; Haruki, Mitsuru; Imanaka, Tadayuki; Morikawa, Masaaki; Kanaya, Shigenori

doi:10.1263/jbb.91.100

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…Total genomic DNA of G. thermoleovorans B23 was prepared as described previously [25] and was partially digested with Sau 3AI. Then the DNA fragments were ligated with phage vector Lambda EMBL3 using Lambda EMBL3/ Bam HI arm (Promega, Madison, WI) and packaged in vitro by Maxplax packaging extract kit (Epicentre Technologies, Madison, WI).…”

Section: Methodsmentioning

confidence: 99%

Alkane inducible proteins in Geobacillus thermoleovorans B23

et al. 2009

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BackgroundInitial step of β-oxidation is catalyzed by acyl-CoA dehydrogenase in prokaryotes and mitochondria, while acyl-CoA oxidase primarily functions in the peroxisomes of eukaryotes. Oxidase reaction accompanies emission of toxic by-product reactive oxygen molecules including superoxide anion, and superoxide dismutase and catalase activities are essential to detoxify them in the peroxisomes. Although there is an argument about whether primitive life was born and evolved under high temperature conditions, thermophilic archaea apparently share living systems with both bacteria and eukaryotes. We hypothesized that alkane degradation pathways in thermophilic microorganisms could be premature and useful to understand their evolution.ResultsAn extremely thermophilic and alkane degrading Geobacillus thermoleovorans B23 was previously isolated from a deep subsurface oil reservoir in Japan. In the present study, we identified novel membrane proteins (P16, P21) and superoxide dismutase (P24) whose production levels were significantly increased upon alkane degradation. Unlike other bacteria acyl-CoA oxidase and catalase activities were also increased in strain B23 by addition of alkane.ConclusionWe first suggested that peroxisomal β-oxidation system exists in bacteria. This eukaryotic-type alkane degradation pathway in thermophilic bacterial cells might be a vestige of primitive living cell systems that had evolved into eukaryotes.

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Section: Methodsmentioning

confidence: 99%

Alkane inducible proteins in Geobacillus thermoleovorans B23

et al. 2009

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“…In fact, there are many examples of biological relevance for chemotactically coupled predator-prey microorganisms. To name just a few, common microbial predators and phagocytes are Bdellovibrio [4][5][6], P. aeruginosa [7], D. discoideum [8,9], lymphocytes [10] and M. xanthus [11,12].…”

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confidence: 99%

Chemotactic predator-prey dynamics

2011

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A discrete chemotactic predator-prey model is proposed in which the prey secrets a diffusing chemical which is sensed by the predator and vice versa. Two dynamical states corresponding to catching and escaping are identified and it is shown that steady hunting is unstable. For the escape process, the predator-prey distance is diffusive for short times but exhibits a transient subdiffusive behavior which scales as a power law t 1/3 with time t and ultimately crosses over to diffusion again. This allows to classify the motility and dynamics of various predatory bacteria and phagocytes. In particular, there is a distinct region in the parameter space where they prove to be infallible predators.PACS numbers: 87.17.Jj,05.10.Gg Phagocytes or predatory microbes are hunting their prey by chemotaxis [1, 2], i.e. they sense the concentration of a chemical which is secreted by the prey and is diffusing through the solution [3]. Typically the predator moves along the steepest gradient of the chemical concentration to ultimately find its ejection source. Likewise the prey (for example another microbe) "smells" a secreted chemical from the advancing predator and tries to escape by moving along in the opposite direction of its maximal gradient. This chemotactically coupled predatorprey systems are relevant for many biological microorganisms. In fact, there are many examples of biological relevance for chemotactically coupled predator-prey microorganisms. To name just a few, common microbial predators and phagocytes are Bdellovibrio [4-6], P. aeruginosa [7], D. discoideum [8,9], lymphocytes [10] and M. xanthus [11,12].Previous theoretical investigations have focussed on spatiotemporal pattern formation in predator-prey colonies [13,14] which are typically described by nonlinear reaction-diffusion equations [15,16]. While the latter approaches involve a coarse-grained continuum modelling, there are much less model studies on individual microorganisms. A discrete swarming model of individual self-propelled particles for bacterial colonies has been proposed by Csirok et al [13]. This was elaborated recently by Romanczuk et al [17] based on a related individual model of . Finally individual autochemotactic models have been studied where the microbe follows its own diffusing secretion [19][20][21][22]. In all of these individual models there is no predator involved, apart from a recent study [23] which addressed a lattice model with no chemicals involved.Here we propose a discrete model which describes both the predator and the prey individually and contains explicitly the diffusion of the two chemicals secreted by the predator and the prey together with the Brownian motion of the latter. The deterministic (fluctuation-free) model is analyzed analytically and by numerical solution which is supplemented by Brownian dynamics computer simulations at finite temperature. Depending on the model parameters and the initial distance between predator and prey, two different dynamical processes are identified which correspond to catching an...

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“…B23 can degrade a broad range of alkanes (C 11 to C 32 ) at 70°C by the terminal oxidation pathway. Despite extensive survey and analysis of related genes and enzymes in this pathway ( 2 – 4 ), the alkane monooxygenase system for initial oxidation of alkane to primary alcohol has remained unknown. In order to elucidate comprehensive alkane degradation pathways in G. thermoleovorans , whole-genome sequence analysis was conducted.…”

Section: Genome Announcementmentioning

confidence: 99%