Gene Delivery Efficacy of Polyethyleneimine-Introduced Chitosan Shell/Poly(methyl Methacrylate) Core Nanoparticles for Rat Mesenchymal Stem Cells

Abstract

This work investigated polyethyleneimine (PEI)-introduced chitosan (CS) (CS/PEI) nanoparticles as non-viral carrier of plasmid DNA for rat mesenchymal stem cells (MSCs). The CS/PEI nanoparticles were prepared by the emulsifier-free emulsion polymerization of methyl methacrylate monomer induced by a small amount of t-butyl hydroperxide in the presence of different concentrations of PEI mixed with CS. The resulting nanoparticles were characterized by their surface properties and buffering capacity. In vitro gene transfection was also evaluated. The introduction of PEI affected the surface charge, dispersing stability and buffering capacity of the nanoparticles. The CS/PEI nanoparticles formed a complex upon mixing with a plasmid DNA of luciferase. The complex enhanced the level of gene transfection and prolonged the time period of expression for MSCs, compared with those of plasmid DNA-original CS and PEI nanoparticles. Cytotoxicity of CS/PEI complexes with plasmid DNA was significantly low, depending on the amount of PEI introduced. It is concluded that the CS/PEI nanoparticle was a promising carrier for gene delivery of MSCs.

The PEI-introduced CS shell/PMMA core nanoparticle for silencing the expression of E6/E7 oncogenes in human cervical cells

“…Therefore, the CS-PEI nanoparticle has promise for use as a carrier for gene delivery. This result is corresponded with the previous study of Pimpha et al (2010).…”

“…The complexes were prepared at various nitrogen/phosphate molar ratios (N/P) depending on the amine group (N) in the nanoparticle and the phosphate group (P) in the DNA or siRNA. The amount of amine groups on CS-PEI surface was determined by conventional 2,4,6-trinitrobenzene sulfonic acid (TNBS) assay as previously described in Pimpha et al (2010).…”

“…Chitosan is a cationic polysaccharide, produced by deacetylation of chitin from crustacean shells. Previously, the gene delivery effectiveness of PEI-introduced chitosan shell/poly(methyl methacrylate) core nanoparticles (CS-PEI nanoparticles) was demonstrated in rat mesenchymal stem cells (Pimpha, Sunintaboon, Inphonlek, & Tabata, 2010). The study concluded that the CS-PEI nanoparticle was a promising carrier for gene delivery.…”

Synthesis of poly(methyl methacrylate) core/chitosan-mixed-polyethyleneimine shell nanoparticles and their antibacterial property

“…PEI was partially protonated by concentrated hydrochloric acid until its pH became 7 to avoid precipitation when mixed with the acidic CS solution. The nanoparticles, having PMMA core with CS, PEI, and CS-mixed-PEI (CS/PEI) shells, were prepared by the emulsifier-free emulsion polymerization [12,24]. The amounts of CS and PEI charged were 0.5 g for the original systems.…”

Lower-Molecular-Weight Chitosan-Treated Polyethyleneimine: a Practical Strategy For Gene Delivery to Mesenchymal Stem Cells

“…For the construction of a chitosan-PEI carrier with high transfection efficiency and low toxicity, Pimpha et al prepared chitosan shell-PEI nanoparticles for gene delivery into MSCs [13]. The complex improved the level of gene transfection and extended the time period for expression in MSCs.…”