Gene Distribution in Cereal Genomes

Gill, Kulvinder S.

doi:10.1007/1-4020-2359-6_12

Cited by 10 publications

(20 citation statements)

References 85 publications

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“…However, in an earlier investigation GILL et al [70] characterised the protein as aleuron protein, similar to other 11/12-S seed storage proteins. The isolated protein was described by the authors as ,,morula-type", an almost globular aggregate with a particle diameter of 12 nm.…”

Section: Introductionmentioning

confidence: 72%

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Rapeseed: Constituents and protein products Part I. Composition and properties of proteins and glucosinolates

Mieth

Schwenke

Raab

et al. 1983

Nahrung

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Section: Introductionmentioning

confidence: 72%

“…Contrary to these, GILL et al [70] found that the 12-S protein from B. camp. contains 12.9 % carbohydrate.…”

Section: Introductionmentioning

confidence: 86%

Rapeseed: Constituents and protein products Part I. Composition and properties of proteins and glucosinolates

Mieth

Schwenke

Raab

et al. 1983

Nahrung

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“…About 60 % of the genes are present in the distal one-third of the chromosomes Sidhu and Gill, 2004). It has also been shown that the size of GRRs decreases and the number of GRRs increases, as the genome size increases from rice to wheat (Gill KS, 2004). For instance, the average size of gene clusters in rice is F 300 kb compared to less than 50 kb in wheat and barley.…”

Section: Gene-rich and Gene-empty Regions In The Wheat Genomementioning

confidence: 96%

“…It has been shown that within the genome, genes are not distributed randomly and that there are GRRs and gene-empty regions not only within the wheat genome, but perhaps in all eukaryotes (for a review, see Gill KS, 2004;Sidhu and Gill, 2004).…”

Section: Gene Distribution In Wheatmentioning

confidence: 99%

“…It may be because the estimations of average recombination rates in rice were at F 400 kb resolution (in wheat the resolution is much lower than in rice), whereas the recombination hotspots may be only !1 kb in length. Due to averaging over a larger region, recombination in hot spots in rice and wheat may appear to be low relative to that in yeast (for references, see Gill KS, 2004;Sidhu and Gill, 2004).…”

Section: Variable Recombination Rates In the Wheat Genomementioning

confidence: 99%

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Wheat cytogenetics in the genomics era and its relevance to breeding

Gupta

Kulwal

Rustgi³

2005

Cytogenet Genome Res

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Hexaploid wheat is a species that has been subjected to most extensive cytogenetic studies. This has contributed to understanding the mechanism of the evolution of polyploids involving diploidization through genetic restriction of chromosome pairing to only homologous chromosomes. The availability of a variety of aneuploids and the ph mutants (Ph1 and Ph2) in bread wheat also allowed chromosome manipulations leading to the development of alien addition/substitution lines and the introgression of alien chromosome segments into the wheat genome. More recently in the genomics era, molecular tools have been used extensively not only for the construction of molecular maps, but also for identification/isolation of genes/QTLs (including epistatic QTLs, eQTLs and PQLs) for several agronomic traits. It has also been possible to identify gene-rich regions and recombination hot spots in the wheat genome, which are now being subjected to sequencing at the genome level, through development of BAC libraries. In the EST database also, among all plants wheat ESTs are the highest in number, and are only next to those for human, mouse, Ciona intestinalis (a chordate), rat and zebrafish genomes. These ESTs and sequences of several genomic regions have been subjected to a variety of applications including development of perfect markers and establishment of microcollinearity. The technique of in situ hybridization (including FISH, GISH and McFISH) and the development of deletion stocks also facilitated the preparation of physical maps. Molecular markers are also used for marker-assisted selection in wheat breeding programs in several countries. Construction of a wheat DNA chip, which will also become available soon, may further facilitate wheat genomics research. These enormous resources, knowledge base and the fast development of additional molecular tools and high throughput approaches for genotyping will prove extremely useful in future wheat research and will lead to development of improved wheat cultivars.

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The structure of the 12 S globulin from rapeseed (Brassica napus L.)

Schwenke¹,

Raab²,

Plietz

et al. 1983

Nahrung

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The 12 S globulin of rapeseed represents an oligomeric protein with a molecular weight of 300,000. It is composed of 6 subunits, which are arranged in a trigonal. antiprism with the point group symmetry 32 (D3).Each subunit contains smaller units (polypeptide chains) with molecular weights in the range of 18,500 to 31,000. The protein contains the following 4 polypeptide chains differing by their molecular weights in the SDS-electrophoresis: 18,500 k 800, 21,100 500, 26,800 900, 31,200 k 1,600.According to its quaternary structure the protein dissociates under milieu conditions : low ionic strength extremely high or low pHThe secondary structure of the protein is characterized by a low (1 1 yo) content of a-helix and a relatively high (31 %) content of &conformation.11/12 S globulins in the seeds of different plant species and botanical families.Owing to its structure and physico-chemical properties the rapeseed protein is closely related to otherThe 12 S globulin represents a main storage protein in the seeds of Brassica species [I -61. 21-33% of the nitrogen in sodium chloride extracts of defatted rapeseed varieties or 18-28The globulin was first isolated by BHATTY et al.[I] from oil-free rapeseed meal by extraction with 10 % sodium chloride, precipitation by dialysis against water, and chromatographic separation on Sephadex G-100. SIMARD et al. 191 used the technique of fractionating precipitation and dissolution by ammonium sulphate for the isolation of the protein. SCHWENKE et al. [lo] described a combined gel and ionic-exchange chromatographic purification of the 12 S globulin. The present paper summarizes the results of research on the physico-chemical properties and structure of the 12 S globulin of rapeseed. of the total seed nitrogen correspond to this protein [l, 7, 81. Hydrodynamic properties and molecular weightAfter isolation and purification of the protein BHATTY et al.[l] could show by ultracentrifugal analysis that this rapeseed globulin, owing to its sedimentation property, corresponds to the group of 11/12 S seed proteins. The authors found a sedimentation coeffi-

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Gene Distribution in Cereal Genomes

Cited by 10 publications

References 85 publications

Rapeseed: Constituents and protein products Part I. Composition and properties of proteins and glucosinolates

Rapeseed: Constituents and protein products Part I. Composition and properties of proteins and glucosinolates

Wheat cytogenetics in the genomics era and its relevance to breeding

The structure of the 12 S globulin from rapeseed (Brassica napus L.)

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