Gene Expression Analysis during Interaction of Tomato and Related Wild Species with Clavibacter michiganensis subsp. michiganensis

Lara-Ávila, José Pablo; Isordia-Jasso, María I.; Castillo-Collazo, Rosalba; Simpson, June; Alpuche-Solís, Ángel G.

doi:10.1007/s11105-011-0348-8

Cited by 21 publications

(18 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While resistant plants initiate responses in incompatible interactions, susceptible plants can also launch a series of basal defense responses in compatible interactions. Although they present similar expression profiles, defense gene induction in compatible interactions occurs later than that in incompatible interactions (Balaji et al 2008;Lara-Ávila et al 2012). BLAST2GO analysis was performed for genes up-regulated at 12 and 24 hai in both resistant and susceptible genotypes (Fig.…”

Section: Profiling Of Differentially Expressed Genes (Degs)mentioning

confidence: 99%

High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection

et al. 2017

View full text Add to dashboard Cite

We provide a transcriptional profile of coffee rust interaction and identified putative up regulated resistant genes Coffee rust disease, caused by the fungus Hemileia vastatrix, is one of the major diseases in coffee throughout the world. The use of resistant cultivars is considered to be the most effective control strategy for this disease. To identify candidate genes related to different mechanism defense in coffee, we present a time-course comparative gene expression profile of Caturra (susceptible) and Híbrido de Timor (HdT, resistant) in response to H. vastatrix race XXXIII infection. The main objectives were to obtain a global overview of transcriptome in both interaction, compatible and incompatible, and, specially, analyze up-regulated HdT specific genes with inducible resistant and defense signaling pathways. Using both Coffea canephora as a reference genome and de novo assembly, we obtained 43,159 transcripts. At early infection events (12 and 24 h after infection), HdT responded to the attack of H. vastatrix with a larger number of up-regulated genes than Caturra, which was related to prehaustorial resistance. The genes found in HdT at early hours were involved in receptor-like kinases, response ion fluxes, production of reactive oxygen species, protein phosphorylation, ethylene biosynthesis and callose deposition. We selected 13 up-regulated HdT-exclusive genes to validate by real-time qPCR, which most of them confirmed their higher expression in HdT than in Caturra at early stage of infection. These genes have the potential to assist the development of new coffee rust control strategies. Collectively, our results provide understanding of expression profiles in coffee-H. vastatrix interaction over a time course in susceptible and resistant coffee plants.

show abstract

Section: Profiling Of Differentially Expressed Genes (Degs)mentioning

confidence: 99%

High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection

et al. 2017

View full text Add to dashboard Cite

show abstract

“…While resistant plants initiate responses in incompatible interactions, susceptible plants can also launch a series of basal defense responses in compatible interactions. Although they present similar expression profiles, defense gene induction in compatible interactions occurs later than that in incompatible interactions ( Tao et al, 2003 ; Li et al, 2006 ; Balaji et al, 2008 ; Lara-Ávila et al, 2012 ). In the compatible chickpea– Ascochyta interaction, most of the genes analyzed were rapidly induced and transcriptionally up-regulated 1 dpi.…”

Section: Discussionmentioning

confidence: 97%

Transcriptome analysis of the compatible interaction of tomato with Verticillium dahliae using RNA-sequencing

et al. 2015

View full text Add to dashboard Cite

Tomato Verticillium wilt is a soil-borne vascular disease caused by the necrotrophic fungus Verticillium dahliae. Although some understanding of plant defense mechanisms against V. dahliae infection has been gained for incompatible interactions, including identification of inducible resistant genes and defense signaling pathways, the genes and signaling pathways involved in the compatible interaction remain unclear. To investigate the molecular basis of the compatible interaction between tomato and V. dahliae, transcriptomes of V. dahliae infected tomatoes were compared to those of a control group. A total of approximately 25 million high-quality reads were generated by means of the RNA sequencing (RNA-seq) method. The sequence reads were aligned to the tomato reference genome and analyzed to measure gene expression levels, and to identify alternative splicing events. Comparative analysis between the two samples revealed 1,953 significantly differentially expressed genes (DEGs), including 1,281 up-regulated and 672 down-regulated genes. The RNA-Seq output was confirmed using RT-qPCR for 10 selected genes. The Nr, Swiss-Prot, Gene Ontology (GO), Clusters of Orthologous Groups (COG), and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases were used to annotate DEG functions. Of the 1,953 DEGs identified, 1,953, 1,579, 1,739, 862, and 380 were assigned by Nr, Swiss-Prot, GO, COG, and KEGG, respectively. The important functional groups identified via GO and COG enrichment were those responsible for fundamental biological regulation, secondary metabolism, and signal transduction. Of DEGs assigned to 87 KEGG pathways, most were associated with phenylpropanoid metabolism and plant–pathogen interaction pathways. Most of the DEGs involved in these two pathways were up-regulated, and may be involved in regulating the tomato-V. dahliae compatible interaction. The results will help to identify key susceptible genes and contribute to a better understanding of the mechanisms of tomato susceptible response to V. dahliae.

show abstract

“…Plant pathogens face a multifaceted defence response upon entering host plants, including the induction of defence‐related genes, production and scavenging of free oxygen radicals, enhanced protein turnover and hormone synthesis. These are triggered when Cmm infects tomato plants (Balaji et al ., ; Lara‐Ávila et al ., ). Therefore successful plant infection requires that phytopathogens overcome such defence mechanisms (Flores‐Cruz & Allen, ).…”

Section: Discussionmentioning

confidence: 97%

Induction of the viable but nonculturable state in Clavibacter michiganensis subsp. michiganensis and in planta resuscitation of the cells on tomato seedlings

Jiang

et al. 2015

Plant Pathology

View full text Add to dashboard Cite

Bacterial canker of tomato is an economically important seedborne disease caused by Clavibacter michiganensis subsp. michiganensis (Cmm). Copper‐based bactericides and seed treatment with hydrochloric acid are commonly used for bacterial canker management. Recent studies have shown that some bacteria can enter a viable but nonculturable (VBNC) state, and fail to form colonies on microbiological agar media. Bacteria in the VBNC state can recover their culturability when returned to favourable conditions. This study reports the induction of the VBNC state in Cmm by CuSO4 and low pH, and resuscitation of VBNC cells on tomato seedlings. Flow cytometry using the nucleic acid dyes SYTO 9 and propidium iodide, combined with agar plating, was used to assess VBNC cell counts. It was demonstrated that CuSO4 and low pH induced the VBNC state in Cmm and the rate of induction increased with copper ion concentration and acidity. Pathogenicity tests showed that some of the VBNC cells induced by CuSO4 retained their ability to colonize tomato seedlings but failed to produce typical bacterial canker symptoms by 2 months post‐inoculation. This was probably due to low levels of resuscitation of VBNC Cmm cells resulting in low levels of initial inoculum. This study has improved understanding of the VBNC state of Gram‐positive phytopathogenic bacteria. Most importantly, because copper‐based chemicals and low pH conditions are used for disease management, induction of the VBNC state and subsequent resuscitation of Cmm cells on tomato seedlings may limit pathogen detection by culture‐based assays yet present a risk for disease development in the field.

show abstract

Gene Expression Analysis during Interaction of Tomato and Related Wild Species with Clavibacter michiganensis subsp. michiganensis

Cited by 21 publications

References 62 publications

High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection

High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection

Transcriptome analysis of the compatible interaction of tomato with Verticillium dahliae using RNA-sequencing

Induction of the viable but nonculturable state in Clavibacter michiganensis subsp. michiganensis and in planta resuscitation of the cells on tomato seedlings

Contact Info

Product

Resources

About