Gene Expression Analysis of Alfalfa Seedlings Response to Acid-Aluminum

Zhou, Peng; Su, Liantai; Lv, Aimin; Wang, Shengyin; Huang, Bingru; An, Yuan

doi:10.1155/2016/2095195

Cited by 27 publications

(20 citation statements)

References 43 publications

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“…Meanwhile, CA10g12810 , which is homologous to Arabidopsis thaliana APL , also encodes a MYB-family TF APL predicted to participate in the regulation of anthocyanin metabolism (GO:0031537). Further, Zhou et al (2016) reported that APL was also involved in phenylpropanoid and flavonoid biosynthesis pathways [ 52 ]. CA10g12710 , a homolog of nol10 in Danio rerio was predicted to be a WD40 repeat protein based on its COG annotation.…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, we may answer the question rasied by Zhang et al (2015) that MYB39 might take part in phenylpropanoid pathway [ 75 ]. Zhou et al (2016) also reported that APL took part in phenylpropanoid and flavonoid biosynthesis pathways [ 52 ]. CA10g03650 and CA10g12810 were different from A locus because A locus was homologous to tomato ANT1 and petunia AN2 [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

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Genetic mapping of anthocyanin accumulation-related genes in pepper fruits using a combination of SLAF-seq and BSA

Wang

Chen

et al. 2018

PLoS ONE

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Anthocyanins have significant functions in stress tolerance in pepper (Capsicum annuum L.) and also benefit human health. Nevertheless, the key structural genes and regulatory genes involved in anthocyanin accumulation in pepper fruits are still not well understood and fine mapped. For the present study, 383 F2 plants from a cross between the green-fruited C. annuum line Z5 and the purple-fruited line Z6 was developed. Two separate bulked DNA pools were constructed with DNAs extracted from either 37 plants with high anthocyanin content or from 18 plants with no anthocyanin. A combination of specific-locus amplified fragment sequencing (SLAF-seq) and bulked segregant analysis (BSA) was used to identify candidates for regions associated with anthocyanin accumulation. We identified a total of 127,004 high-quality single nucleotide polymorphism (SNP) markers, and detected 1674 high-quality SNP markers associated with anthocyanin accumulation. Three candidate anthocyanin-associated regions including the intervals from 12.48 to 20.00 Mb, from 54.67 to 56.59 Mb, and from 192.17 to 196.82 Mb were identified within a 14.10-Mb interval on chromosome 10 containing 126 candidate genes. Based on their annotations, we identified 12 candidate genes that are predicted to be associated with anthocyanin expression. The present results provide an efficient strategy for genetic mapping of and valuable insights into the genetic mechanisms of anthocyanin accumulation in pepper fruit, and allow us to clone and functionally analyze the genes that influence anthocyanin accumulation in this species.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genetic mapping of anthocyanin accumulation-related genes in pepper fruits using a combination of SLAF-seq and BSA

Wang

Chen

et al. 2018

PLoS ONE

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“…Al toxicity severely restricts the growth of alfalfa. In our previous study of transcriptome‐wide analysis of Al‐stressed alfalfa seedlings, six LEA genes were found to be upregulated under Al stress (Zhou et al., 2016). Upon overexpressing the LEA genes in Arabidopsis, abiotic stress (drought, salt and Al) tests showed that an SK 3 ‐type DHN MsDHN1 significantly increased the Al tolerance of transgenic Arabidopsis.…”

Section: Introductionmentioning

confidence: 97%

Dehydrin MsDHN1 improves aluminum tolerance of alfalfa (Medicago sativaL.) by affecting oxalate exudation from root tips

Wen

Fan

et al. 2021

The Plant Journal

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SUMMARY A SK3‐type dehydrin MsDHN1 was cloned from alfalfa (Medicago sativa L.). Its function and gene regulatory pathways were studied via overexpression and suppression of MsDHN1 in alfalfa seedlings or hairy roots. The results showed that MsDHN1 is a typical intrinsically disordered protein that exists in the form of monomers and homodimers in alfalfa. The plant growth rates increased as a result of MsDHN1 overexpression (MsDHN1‐OE) and decreased upon MsDHN1 suppression (MsDHN1‐RNAi) in seedlings or hairy roots of alfalfa compared with the wild‐type or the vector line under Al stress. MsDHN1 interacting with aquaporin (AQP) MsPIP2;1 and MsTIP1;1 positively affected oxalate secretion from root tips and Al accumulation in root tips. MsABF2 was proven to be an upstream transcription factor of MsDHN1 and activated MsDHN1 expression by binding to the ABRE element of the MsDHN1 promoter. The transcriptional regulation of MsABF2 on MsDHN1 was dependent on the abscisic acid signaling pathway. These results indicate that MsDHN1 can increase alfalfa tolerance to Al stress via increasing oxalate secretion from root tips, which may involve in the interaction of MsDHN1 with two AQP.

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“…MYB and NAC TFs were majorly involved in the tolerance mechanism in the case of cultivars, whereas in the case of wild, WRKY and bHLH TFs were majorly involved. Zhou et al ( 2016 ) found that MYB and WRKY TFs were involved in the regulation of ROS and flavonoid biosynthesis under Al 3+ stress conditions in alfalfa. Similarly, zinc finger, MYB, and WRKY TFs, together, played critical roles in Al 3+ stress resilience in tea (Huang et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative Inter- and IntraSpecies Transcriptomics Revealed Key Differential Pathways Associated With Aluminium Stress Tolerance in Lentil

Singh

Chaudhary

et al. 2021

Front. Plant Sci.

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Aluminium stress causes plant growth retardation and engenders productivity loss under acidic soil conditions. This study accentuates morpho-physiological and molecular bases of aluminium (Al) tolerance within and between wild (ILWL-15) and cultivated (L-4602 and BM-4) lentil species. Morpho-physiological studies revealed better cyto-morphology of tolerant genotypes over sensitive under Al3+ stress conditions. Mitotic lesions were observed in root cells under these conditions. Transcriptome analysis under Al3+ stress revealed 30,158 specifically up-regulated genes in different comparison groups showing contigs between 15,305 and 18,861 bp. In tolerant genotypes, top up-regulated differentially expressed genes (DEGs) were found to be involved in organic acid synthesis and exudation, production of antioxidants, callose synthesis, protein degradation, and phytohormone- and calcium-mediated signalling under stress conditions. DEGs associated with epigenetic regulation and Al3+ sequestration inside vacuole were specifically upregulated in wild and cultivars, respectively. Based on assembled unigenes, an average of 6,645.7 simple sequence repeats (SSRs) and 14,953.7 high-quality single nucleotide polymorphisms (SNPs) were spotted. By quantitative real-time polymerase chain reaction (qRT-PCR), 12 selected genes were validated. Gene ontology (GO) annotation revealed a total of 8,757 GO terms in three categories, viz., molecular, biological, and cellular processes. Kyoto Encyclopaedia of Genes and Genomes pathway scanning also revealed another probable pathway pertaining to metacaspase-1,−4, and −9 for programmed cell death under Al-stress conditions. This investigation reveals key inter- and intraspecies metabolic pathways associated with Al-stress tolerance in lentil species that can be utilised in designing future breeding programmes to improve lentil and related species towards Al3+ stress.

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Gene Expression Analysis of Alfalfa Seedlings Response to Acid-Aluminum

Cited by 27 publications

References 43 publications

Genetic mapping of anthocyanin accumulation-related genes in pepper fruits using a combination of SLAF-seq and BSA

Genetic mapping of anthocyanin accumulation-related genes in pepper fruits using a combination of SLAF-seq and BSA

Dehydrin MsDHN1 improves aluminum tolerance of alfalfa (Medicago sativaL.) by affecting oxalate exudation from root tips

Comparative Inter- and IntraSpecies Transcriptomics Revealed Key Differential Pathways Associated With Aluminium Stress Tolerance in Lentil

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