Gene Expression and Microcomputed Tomography Analysis of Grafted Bone Using Deproteinized Bovine Bone and Freeze-Dried Human Bone

Kangwannarongkul, Thanyaporn; Subbalekha, Keskanya; Vivatbutsiri, Philaiporn; Suwanwela, Jaijam

doi:10.11607/jomi.6234

Cited by 14 publications

(20 citation statements)

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“…These cells contain MSCs that could differentiate into osteoblasts and cementoblasts [8,44]. In the previous studies, DBBM induced a high vascularization in mouse connective tissue at 10 days after implantation [45], and the gene expression of Osx in mouse periodontal defects at 3 months [46]. In the current study, in all areas, the proportions of PCNA-, VEGF-, and Osx-positive cells in the FGF-2 + DBBM group were significantly greater than that in the Unfilled group at 2 and 4 weeks.…”

Section: Discussionmentioning

confidence: 99%

Healing of Experimental Periodontal Defects Following Treatment with Fibroblast Growth Factor-2 and Deproteinized Bovine Bone Mineral

et al. 2021

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The aim of this study was to investigate the effects of fibroblast growth factor (FGF)-2 used in combination with deproteinized bovine bone mineral (DBBM) on the healing of experimental periodontal defects. Periodontal defects created in rats were treated by FGF-2, DBBM, FGF-2 + DBBM, or left unfilled. Microcomputed tomography, histological, and immunohistochemical examinations were used to evaluate healing. In vitro cell viability/proliferation on DBBM with/without FGF-2 was assessed by WST-1. Cell behavior was analyzed using scanning electron and confocal laser scanning microscopy. Osteogenic differentiation was evaluated by staining with alkaline phosphatase and alizarin red. Bone volume fraction was significantly greater in FGF-2 and FGF-2 + DBBM groups than in other groups at 2 and 4 weeks postoperatively. In histological assessment, newly formed bone in FGF-2 and FGF-2 + DBBM groups appeared to be greater than other groups. Significantly greater levels of proliferating cell nuclear antigen-, vascular endothelial growth factor-, and osterix-positive cells were observed in FGF-2 and FGF-2 + DBBM groups compared to Unfilled group. In vitro, addition of FGF-2 to DBBM promoted cell viability/proliferation, attachment/spreading, and osteogenic differentiation. The combination therapy using FGF-2 and DBBM was similarly effective as FGF-2 alone in the healing of experimental periodontal defects. In certain bone defect configurations, the combined use of FGF-2 and DBBM may enhance healing via promotion of cell proliferation, angiogenesis, and osteogenic differentiation.

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Section: Discussionmentioning

confidence: 99%

Healing of Experimental Periodontal Defects Following Treatment with Fibroblast Growth Factor-2 and Deproteinized Bovine Bone Mineral

et al. 2021

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“…Ivanovski et al (2011), in a similar model, showed overexpression of genes associated with skeletal growth and ossification (such as BMP2, BMP3, TGFβ, osteocalcin, osteomodulin, and Runx2, Sox6, Satb2 transcription factors) 14 d after regeneration. Recently, Kangwannarongkul et al (2018) showed that at 1 and 3 mo following bovine bone augmentation of calvaria defects in mice, an elevation of selected bone osteogenic genes such as Runx2 and ALP was observed. These studies, as well as the current study, indicate that active bone turnover ensues at the early phase of healing (as shown by Suleimenova et al, 2017, and Ivanovski et al, 2011) and continues for a long period (Kangwannarongkul et al, 2018, and the current study).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, Kangwannarongkul et al (2018) showed that at 1 and 3 mo following bovine bone augmentation of calvaria defects in mice, an elevation of selected bone osteogenic genes such as Runx2 and ALP was observed. These studies, as well as the current study, indicate that active bone turnover ensues at the early phase of healing (as shown by Suleimenova et al, 2017, and Ivanovski et al, 2011) and continues for a long period (Kangwannarongkul et al, 2018, and the current study). The augmented multiple inflammatory pathways found in the GSEA analysis in the bovine bone group might reflect immunogenic traits of the bone particles (Supronowicz et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Bovine Bone Promotes Osseous Protection via Osteoclast Activation

et al. 2020

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The current study aimed at investigating the long-term biological mechanisms governing bone regeneration in osseous defects filled with bovine bone (BB). Tooth extraction sockets were filled with BB or left unfilled for natural healing in a C57BL/6 mouse alveolar regeneration bone model ( n = 12). Seven weeks later, the alveolar bone samples were analyzed histologically with hematoxylin/eosin and tartrate-resistant acid phosphatase staining. A separate group ( n = 10) was used for RNA sequencing. Osteoclast inhibition was induced by zoledronic acid (ZA) administration at 2 wk postextraction in a third group ( n = 28) for examination of osseous changes and cellular functions with micro–computed tomography and quantitative reverse transcription polymerase chain reaction, respectively. Histological and radiological osseous healing was observed in both BB-filled and normal-healing sockets. However, BB regenerated bone showed significant robust expression of genes associated with bone homeostasis and osteoclasts’ function. Osteoclasts’ inhibition in BB-filled sockets led to decreased bone resorption markers and reduced bone formation to a greater extent than that observed in osteoclasts’ inhibition with natural healing. BB displays long-term biologically active properties, despite a naive osseous histological appearance. These include activation of osteoclasts, which in turn promotes osseous remodeling and maturation of ossified bone.

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“…In a rabbit experiment using bone allograft for critical size defects, an increasing immunostaining for these markers was found and those staining patterns were similar to our findings. These osteogenic markers were also upregulated in a mouse calvarium defect model using human DFDBA, where gene expression was investigated after 1 and 3 months of healing . From allograft studies in rhesus monkeys, it is known that non‐decalcified freeze‐dried human allograft is superior to decalcified allografts in stimulating new bone formation .…”

Section: Discussionmentioning

confidence: 99%

“…These osteogenic markers were also upregulated in a mouse calvarium defect model using human DFDBA, where gene expression was investigated after 1 and 3 months of healing. 54 From allograft studies in rhesus monkeys, it is known that non-decalcified freeze-dried human allograft is superior to decalcified allografts in stimulating new bone formation. 55 OC-immunoreactive cells were also observed after application of fresh frozen bone for sinus lift after 6 months.…”

Section: Discussionmentioning

confidence: 99%

Histological and immunohistochemical comparison of two different allogeneic bone grafting materials for alveolar ridge reconstruction: A prospective randomized trial in humans

Solakoglu

Götz

Heydecke

et al. 2019

Clin Implant Dent Rel Res

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BackgroundPreclinical studies have hypothesized a possible immunological reponse to allogeneic materials due to detection of remnants of potential immunogenic molecules. However, their impact on integration, bone remodeling and immunological reaction after the augmentation procedure is largely unknown and a direct correlation of analytical data and evaluation of human biopsies is missing.PurposeThe present study aimed to compare two commercially available allogeneic materials regarding their content of cellular remnants as well as the bone remodeling, and integration and potential immunologic reactions on a histological and immunohistochemical level, integrating also in vitro analytical evaluation of the specific batches that were used clinically.Materials and MethodsTwenty patients were randomly assigned to treatment with Maxgraft or Puros for lateral ridge augmentation in a two‐stage surgery. After a mean healing period of 5 months, implants were placed and biopsies were taken for histological, immunhistochemical, and histomorphometrical evaluation regarding bone remodeling and inflammation, protein concentrations in vitro and the presence of MHC molecules of the same batches used clinically.ResultsNo differences in clinical outcome, histological, immunohistochemical, and in vitro protein analysis between the two bone grafting materials were observed. Active bone remodeling, amount of newly formed bone, and residual grafting material was independent of the materials used, but varied between subjects. MHC1 residues were not detected in any sample.ConclusionsWithin the limitations of this study, both tested materials yielded equivalent results in terms of clinical outcome, new bone formation, and lack of immunological potential on a histological and immunohistochemical level.

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Gene Expression and Microcomputed Tomography Analysis of Grafted Bone Using Deproteinized Bovine Bone and Freeze-Dried Human Bone

Abstract: These results showed that both bone graft materials promoted bone regeneration. Bio-Oss demonstrated high osteoconductive properties.

Cited by 14 publications

References 0 publications

Healing of Experimental Periodontal Defects Following Treatment with Fibroblast Growth Factor-2 and Deproteinized Bovine Bone Mineral

Healing of Experimental Periodontal Defects Following Treatment with Fibroblast Growth Factor-2 and Deproteinized Bovine Bone Mineral

Bovine Bone Promotes Osseous Protection via Osteoclast Activation

Histological and immunohistochemical comparison of two different allogeneic bone grafting materials for alveolar ridge reconstruction: A prospective randomized trial in humans

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