2009
DOI: 10.1111/j.1365-2559.2009.03470.x
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Gene expression in colorectal neoplasia: modifications induced by tissue ischaemic time and tissue handling protocol

Abstract: Reliable interpretation of results of gene expression at the mRNA level requires standardized protocols for tissue procurement.

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Cited by 46 publications
(57 citation statements)
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“…Nevertheless, based on the literature, the major tumors of breast, colorectum, liver, and prostate are unlikely to have major molecular damage secondary to typical times of cold ischemia. 3,6,[8][9][10][11][12][13][14][15][16][17][18] Effects of Cold Ischemia of RNA Stability RNA integrity number (RIN) is a surrogate measure of mRNA stability. RIN specifically is an indication of the stability of ribosomal RNA (rRNA) based on the two major peaks of rRNA, 18S and 28S.…”
Section: Cold Ischemiamentioning
confidence: 99%
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“…Nevertheless, based on the literature, the major tumors of breast, colorectum, liver, and prostate are unlikely to have major molecular damage secondary to typical times of cold ischemia. 3,6,[8][9][10][11][12][13][14][15][16][17][18] Effects of Cold Ischemia of RNA Stability RNA integrity number (RIN) is a surrogate measure of mRNA stability. RIN specifically is an indication of the stability of ribosomal RNA (rRNA) based on the two major peaks of rRNA, 18S and 28S.…”
Section: Cold Ischemiamentioning
confidence: 99%
“…Of note, the stability of mRNA is assumed to be very similar to RIN or the 28S:18S rRNA ratio, but this has not been studied adequately and likely varies with a specific transcript and organ system. 3,[10][11][12][13][14][15][16][17] Importantly, most studies of RIN or 28S:18S ratios are performed with specimens held at room temperature (usually assumed to be 22°C-25°C) and some studies of the effects of cold ischemia focus only on the cold ischemia period after specimens leave the operating room and/or arrive in surgical pathology and do not consider the period of cold ischemia in the operating room or during transport. 19 More importantly, most studies do not evaluate the effects of warm ischemia, which is likely to interact with the effects of cold ischemia.…”
Section: Cold Ischemiamentioning
confidence: 99%
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