Gene expression of glucose transporter (GLUT) 1, 3 and 4 in bovine follicle and corpus luteum

Nishimoto, Hiromi; Matsutani, R; Yamamoto, Shinya; Takahashi, Tetsuya; Hayashi, Kanako; Miyamoto, Akio; Hamano, Seizo; Tetsuka, Masafumi

doi:10.1677/joe.1.06210

Cited by 90 publications

(67 citation statements)

References 24 publications

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“…In bovine oocytes and embryos, glucose is the energy source and its uptake occurs through glucose transporters Glut1 (preimplantation periods) and Glut3 (post-compaction) [50][51][52]. In the present study, the relative mRNA abundance of Glut1, Glut3 and IGF1R decreased (p<0.05) in the buffalo oocytes matured at 40.5 and 41.5°C for two time periods (first 12 h or complete 24 h) compared to the oocytes matured at 38.5°C for 24 h (control).…”

Section: Discussionmentioning

confidence: 99%

Developmental competence and expression pattern of bubaline (Bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro

Ashraf

Shah

Saini

et al. 2014

J Assist Reprod Genet

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Objective To determine the direct effect of physiologically relevant high temperatures (40.5 and 41.5°C) for two time periods (12 and 24 h) on bubaline oocytes during in vitro maturation. Method The control group oocytes were cultured at 38.5°C for 24 h. The treatment 1 (T1) and 3 (T3) group oocytes were cultured at 40.5 and 41.5°C respectively, for the first 12 h and at 38.5°C for rest of the 12 h. However, treatment 2 (T2) and 4 (T4) group oocytes were cultured at 40.5 and 41.5°C for complete 24 h. Results Development of oocytes to blastocyst was severely compromised (p<0.001) when matured at 40.5 and 41.5°C for both exposure periods (12 h and 24 h). It was found that the cleavage rates, blastocyst yield and mean cell number decreased remarkably (p<0.001) in the treatment groups compared to control. The relative mRNA expression of heat shock protein (Hsp 70.1, 70.2, 70.8, 60, 10 and HSF1), pro-apoptotic (caspases-3, −7, −8, Bid and Bax) and oxidative stress (iNOS) related genes was significantly higher (p<0.05) in all the treatment groups compared to control. However, mRNA abundance of anti-apoptotic (Bcl-2, Mcl-1, Bcl-xl), glucose transport (Glut1, Glut3 and IGF1R), developmental competence (ZAR1 and BMP15) and oxidative stress (MnSOD) related genes was significantly decreased (p<0.05) in the treatment groups compared to control. Conclusion The present study clearly establishes that physiologically relevant elevated temperatures during in vitro meiotic maturation reduce developmental competence of bubaline oocytes.

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Section: Discussionmentioning

confidence: 99%

Developmental competence and expression pattern of bubaline (Bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro

Ashraf

Shah

Saini

et al. 2014

J Assist Reprod Genet

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“…In small follicle, the significant increase in the glucose concentration may be due to lack of its metabolism and consumption by the few numbers of granular cells in compare to large follicles (Nandi et al, 2007;Leroy et al, 2004). However, other researcher found that high permeability of blood vessel-follicle wall barriers during the follicular growth led to filtrate more glucose from blood plasma into follicular fluid (Ying et al, 2011;Nishimoto et al, 2006). Moreover, Nishimoto et al (2006) described the importance of glucose concentration in the growth media necessary for in vitro development and maturity of eggs.…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

Study the Effects of Follicular Size on some Biochemical Follicular Fluid Composition in She Camel (Camelus Dromedarius)

Hassan¹,

AL-Nuaimi²,

Al-Yasari³

et al. 2018

Adv. Anim. Vet. Sci.

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| This study was designed to estimate the biochemical composition of ovary follicular fluid in relation to its size variations in local Iraqi She camels (Camelus dromedarius). Hundred ovary were collected from 50 adult she camel, 4 to 10 years old that slaughtered at Al Najaf abattoir during the breeding season of camelids. The ovaries were transferred immediately to the laboratory in a cold box. Later on, the follicular fluid was collected separately from small and large size follicles, (3-9 mm) and (10-19 mm) respectively. The fluid was kept at -4 0 C for further analysis. The follicular fluid samples were analyzed to estimate the metabolic composition (cholesterol, glucose and total protein), and the ionic compositions (calcium, sodium and potassium). The results of this study revealed significant (P< 0.05) increase inthe glucose and cholesterol concentration, while there were significant decrease in the total protein in large size follicles. Meanwhile, significant increase was seen in the concentration of Na+ and Ca+2 in relation to the size of the follicles. However, significant decrease was occurred in the concentrations of K with increasing follicle size. In conclusion, the results of this study revealed a significant variations in the concentration of the follicular fluid metabolic and the ionic compositions with the variations of its size.

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“…In comparison, cumulus cells express an additional glucose transporter, SLC2A4 (Williams et al 2001, Roberts et al 2004, Nishimoto et al 2006, which has a high affinity for glucose (K m w2-5 mM) and is an insulin-sensitive transporter, so the rate of glucose transportation into cells by SLC2A4 tends to be more reliant on insulin and insulin-like growth factors levels than on glucose concentration (Charron et al 1989).…”

Section: Coc Versus the Oocytementioning

confidence: 99%

The pivotal role of glucose metabolism in determining oocyte developmental competence

Sutton‐McDowall

Gilchrist²,

Thompson³

2010

Reproduction

433

325

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The environment that the cumulus oocyte complex (COC) is exposed to during either in vivo or in vitro maturation (IVM) can have profound effects on the success of fertilisation and subsequent embryo development. Glucose is a pivotal metabolite for the COC and is metabolised by glycolysis, the pentose phosphate pathway (PPP), the hexosamine biosynthesis pathway (HBP) and the polyol pathway. Over the course of oocyte maturation, a large proportion of total glucose is metabolised via the glycolytic pathway to provide substrates such as pyruvate for energy production. Glucose is also the substrate for many cellular functions during oocyte maturation, including regulation of nuclear maturation and redox state via the PPP and for the synthesis of substrates of extracellular matrices (cumulus expansion) and O-linked glycosylation (cell signalling) via the HBP. However, the oocyte is susceptible to glucose concentration-dependent perturbations in nuclear and cytoplasmic maturation, leading to poor embryonic development post-fertilisation. For example, glucose concentrations either too high or too low result in precocious resumption of nuclear maturation. This review will discuss the relevant pathways of glucose metabolism by COCs during in vivo maturation and IVM, including the relative contribution of the somatic and gamete compartments of the COC to glucose metabolism. The consequences of exposing COCs to abnormal glucose concentrations will also be examined, either during IVM or by altered maternal environments, such as during hyperglycaemia induced by diabetes and obesity.Reproduction (2010) 139 685-695

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Gene expression of glucose transporter (GLUT) 1, 3 and 4 in bovine follicle and corpus luteum

Cited by 90 publications

References 24 publications

Developmental competence and expression pattern of bubaline (Bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro

Developmental competence and expression pattern of bubaline (Bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro

Study the Effects of Follicular Size on some Biochemical Follicular Fluid Composition in She Camel (Camelus Dromedarius)

The pivotal role of glucose metabolism in determining oocyte developmental competence

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