Gene Expression of <i>CXCL1</i> (GRO‐α) and <i>EGF</i> by Platelets in Myeloproliferative Neoplasms

Collinson, Ryan J.; Mazza‐Parton, Allegra; Fuller, Kathryn A.; Linden, Matthew D.; Erber, Wendy N.; Guo, Belinda

doi:10.1097/hs9.0000000000000490

Cited by 7 publications

(2 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We found in our study that CCL5 expression by monocytes/macrophages was significantly elevated in ET, which further demonstrates that monocytes/macrophages can promote platelet production by secreting CCL5. Previous studies have also found that EGF transcript levels and cytokine levels from platelets were significantly elevated in ET [ 80 , 81 ]. Our study extends the origin of EGF to monocytes/macrophages in ET.…”

Section: Discussionmentioning

confidence: 90%

Contributions of bone marrow monocytes/macrophages in myeloproliferative neoplasms with JAK2V617F mutation

Fan,

Cao,

Shi

et al. 2023

Ann Hematol

View full text Add to dashboard Cite

The classic BCR-ABL1-negative myeloproliferative neoplasm (MPN) is a highly heterogeneous hematologic tumor that includes three subtypes, namely polycythemia vera (PV), essential thrombocytosis (ET), and primary myelofibrosis (PMF). Despite having the same JAK2 V617F mutation, the clinical manifestations of these three subtypes of MPN differ significantly, which suggests that the bone marrow (BM) immune microenvironment may also play an important role. In recent years, several studies have shown that peripheral blood monocytes play an important role in promoting MPN. However, to date, the role of BM monocytes/macrophages in MPN and their transcriptomic alterations remain incompletely understood. The purpose of this study was to clarify the role of BM monocytes/macrophages in MPN patients with the JAK2 V617F mutation. MPN patients with the JAK2 V617F mutation were enrolled in this study. We investigated the roles of monocytes/macrophages in the BM of MPN patients, using flow cytometry, monocyte/macrophage enrichment sorting, cytospins and Giemsa-Wright staining, and RNA-seq. Pearson correlation coefficient analysis was also used to detect the correlation between BM monocytes/macrophages and the MPN phenotype. In the present study, the proportion of CD163 + monocytes/macrophages increased significantly in all three subtypes of MPN. Interestingly, the percentages of CD163 + monocytes/macrophages are positively correlated with HGB in PV patients and PLT in ET patients. In contrast, the percentages of CD163 + monocytes/macrophages are negatively correlated with HGB and PLT in PMF patients. It was also found that CD14 + CD16 + monocytes/macrophages increased and correlated with MPN clinical phenotypes. RNA-seq analyses demonstrated that the transcriptional expressions of monocytes/macrophages in MPN patients are relatively distinct. Gene expression profiles of BM monocytes/macrophages suggest a specialized function in support of megakaryopoiesis in ET patients. In contrast, BM monocytes/macrophages yielded a heterogeneous status in the support or inhibition of erythropoiesis. Significantly, BM monocytes/macrophages shaped an inflammatory microenvironment, which, in turn, promotes myelofibrosis. Thus, we characterized the roles of increased monocytes/macrophages in the occurrence and progression of MPNs. Our findings of the comprehensive transcriptomic characterization of BM monocytes/macrophages provide important resources to serve as a basis for future studies and future targets for the treatment of MPN patients. Supplementary Information The online version contains supplementary material available at 10.1007/s00277-023-05284-5.

show abstract

Section: Discussionmentioning

confidence: 90%

Contributions of bone marrow monocytes/macrophages in myeloproliferative neoplasms with JAK2V617F mutation

Fan,

Cao,

Shi

et al. 2023

Ann Hematol

View full text Add to dashboard Cite

show abstract

“…n response to our recent paper which identified GRO-a, EGF and Eotaxin as potential biomarkers in chronic phase myeloproliferative neoplasms, 1 Collinson et al explored one of the possible cellular sources of these molecules by measuring levels of transcripts produced (and stored) within platelet granules. 2 Mutant megakaryocytes and platelets have been previously implicated in MPN pathogenesis, including in the fibrotic processes which is associated with poor prognosis. 3,4 In line with our data, high EGF transcript levels were observed in platelets from essential thrombocythemia (ET) and polycythemia vera (PV) patients.…”

mentioning

confidence: 99%