Gene expression profiles of oral soft tissue‐derived fibroblast from healing wounds: correlation with clinical outcome, autophagy activation and fibrotic markers expression

Rojas, Mariana; Ceccarelli, Simona; Gerini, Giulia; Vescarelli, Enrica; Marini, Lorenzo; Marchese, Cinzia; Pilloni, Andrea

doi:10.1111/jcpe.13439

Cited by 9 publications

(16 citation statements)

References 46 publications

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“…In our previous studies [28,29], we demonstrated a downregulation of αSMA and Col1a1 in gingival tissue 24 h after injury, in line with clinical observation of reduced scar formation in this tissue. Instead of this, the alveolar mucosal (M) tissue showed the opposite response, according to the clinical observation of scar tissue repair.…”

Section: Discussionsupporting

confidence: 86%

“…The other two genes that play a role in regulating scar formation in oral tissues, SERPINE1 and TIMP1, were evaluated. Such genes, involved in collagen deposition and fibrosis, were previously shown to remain stable in gingival tissue at 24 h after injury [29]. In our study, we observed an increase of SERPINE1 and TIMP1 in CHX biopsies of both patients (1.6 and 3.0-fold for SERPINE1; 3.4 and 11.8-fold for TIMP1, respectively; Figure 3B,C).…”

Section: Chx Influences the Expression Of Key Genes Involved In Early Wound Healingsupporting

confidence: 77%

“…Chen et al [27] demonstrated that the main transcriptional changes in the wound healing process occur in the first 12-24 h. In fact, we observed significant changes in myofibroblast differentiation, fibrotic markers, and wound healing gene expression of oral soft tissue derived-fibroblasts 24 h after surgery, when compared to baseline [28,29]. In addition, it was demonstrated that until the first 24 h the biofilm is primarily populated by gram-positive cocci, and gram-negative anaerobic bacteria rapidly increase and predominate after 48 h [30,31].…”

Section: Introductionmentioning

confidence: 56%

“…Based on our group's previous results [29], it was still interesting to further investigate the findings based on previously assessed genes related to scar wound healing. Through qRT-PCR analysis, we evaluated the expression of RAC1, TIMP1, and SERPINE1 genes.…”

Section: Discussionmentioning

confidence: 99%

“…We then investigated the effect of CHX on the expression of some previously shown genes in playing a role in the early wound healing process [29], in two out of the three enrolled patients (since in one of the patients the material obtained with the biopsy was not enough to carry out the analysis). We first evaluated RAC1, a member of the Rho family of small GTPases that promotes healing and that has been previously shown to increase in gingival tissue, 24 h after injury [29]. Interestingly, we observed a significant downmodulation of RAC1 expression at 24 h in CHX biopsies of both patients (0.2 and 0.02-fold, respectively) (Figure 3A), thus suggesting that CHX might impair gingival wound healing.…”

Section: Chx Influences the Expression Of Key Genes Involved In Early Wound Healingmentioning

confidence: 99%

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Effect of Chlorhexidine Digluconate in Early Wound Healing of Human Gingival Tissues. A Histological, Immunohistochemical and Biomolecular Analysis

et al. 2021

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Chlorhexidine digluconate (CHX) is considered the gold standard for oral cavity antiseptic treatment. Nevertheless, several in vitro studies have reported detrimental effects in oral tissue repair. The aim of the present study was to evaluate the in vivo effect of post-surgical CHX mouth rinse on gingival tissue (G) 24 h after injury. G biopsies were obtained in three patients 24 h after surgery with the indication of post-surgical 0.12% CHX use and were compared with those obtained from the same patients without any antiseptic use. Changes in collagen production, cell proliferation, and apoptosis were examined by histological and Ki-67/P53 immunohistochemical analysis. Fibrotic markers (COL1A1, αSMA), proapoptotic protein (BAX) expression, and wound healing-related gene modulation (RAC1, SERPINE1, TIMP1) were analyzed by quantitative real-time PCR analysis. CHX was able to reduce cellular proliferation and increase collagen deposition, proapoptotic molecule and fibrotic marker expression, and myofibroblast differentiation, reduce expression of RAC1 and trigger expression of SERPINE1 and TIMP1, showing “scar wound healing response” pattern. This study assessed for the first time the in vivo effects of CHX on gingival tissue. The demonstration of a CHX-induced fibrotic transformation, leading to scar repair, supports the need for new post-surgical clinical protocols based on a strategic and personalized use of CHX.

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Section: Discussionsupporting

confidence: 86%

Section: Chx Influences the Expression Of Key Genes Involved In Early Wound Healingsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Chx Influences the Expression Of Key Genes Involved In Early Wound Healingmentioning

confidence: 99%

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Effect of Chlorhexidine Digluconate in Early Wound Healing of Human Gingival Tissues. A Histological, Immunohistochemical and Biomolecular Analysis

et al. 2021

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Bone Biology

2022

Essential Techniques of Alveolar Bone Augmentation in Implant Dentistry

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The mucosal released‐coronally advanced flap: A novel surgical approach—A case report

Pilloni,

Dell'Olmo

2024

Clin Adv Periodontics

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BackgroundThe coronally advanced flap (CAF) can be a predictable surgical technique for the treatment of gingival recessions. However, the characteristics of the defect (e.g., limited amount of keratinized gingiva or flap tension, etc.) may limit the use of the CAF with a possible requirement of additional surgical interventions (i.e., the use of a tissue graft to be harvested from donor sites or connective tissue substitutes).MethodsA 28‐year‐old woman patient, with no history of periodontal disease, came for referral presenting receding gums as a chief complaint, poor esthetics, and dentinal hypersensitivity at the buccal surface of teeth 11, 12, and 13. Clinically, she presented a thick phenotype with gingival recession type, RT1, with detectable cemento‐enamel junction (A‒) in the second quadrant. To reduce the need of harvesting soft tissue grafts, the amount of cutting of muscles and vessels from the inner portion of the flap and mitigate the postoperative discomfort associated with the CAF, a novel surgical approach is described here using an advanced flap that incorporates an external incision along the mucogingival junction.ResultsThe average root coverage achieved with the novel procedure presented in this case report was 95%, along with an increased amount of keratinized gingiva and minimal postoperative patient's discomfort.ConclusionsThe mucosal released CAF is a promising technique in which the CAF technique alone may not be an indication.Key pointsThis technique has the following advantages: Reduce the need of harvesting soft tissue grafts. Reduce the amount of cutting of muscles and vessels from the inner portion of the flap. Minimal postoperative discomfort for the patient.

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Gene expression profiles of oral soft tissue‐derived fibroblast from healing wounds: correlation with clinical outcome, autophagy activation and fibrotic markers expression

Cited by 9 publications

References 46 publications

Effect of Chlorhexidine Digluconate in Early Wound Healing of Human Gingival Tissues. A Histological, Immunohistochemical and Biomolecular Analysis

Effect of Chlorhexidine Digluconate in Early Wound Healing of Human Gingival Tissues. A Histological, Immunohistochemical and Biomolecular Analysis

Bone Biology

The mucosal released‐coronally advanced flap: A novel surgical approach—A case report

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