Gene Expression Profiling during Wilting in Chickpea Caused by <i>Fusarium oxysporum</i> F. sp. <i>Ciceri</i>

Abstract

<i>Fusarium oxysporum</i> f. sp. <i>ciceri</i> (<i>Foc</i>), one of the most important fungal pathogen of chickpea, is a constant threat to this crop plant. In the present study gene expression analysis of chickpea roots during <i>Foc</i> infection was performed using various approaches. cDNAs derived from total mRNA during infection process of susceptible (JG62)and resistant (Digvijay) cultivars, were amplified using random oligonucleotides. Sequence characterization of differentially expressed transcripts revealed their homology with many plant genes essential for various metabolic functions including defense. Further, expression patterns of specific candidate gene transcripts were analyzed in the <i>Foc</i> inoculated and uninoculated resistant and susceptible chickpea cultivars, on day 6 of infection. Semiquantitative RT-PCR analysis of defense related genes was performed using gene specific oligonucleotides in resistant and susceptible chickpea cultivars. The expression of fungal pathogenesis related genes and their race specific response was determined throughout the course of chickpea-<i>Foc</i> interaction. Temporal expression and race specific response of plant defense related and fungal virulence genes were studied in the resistant and susceptible cultivars of chickpea inoculated with three races of <i>Foc</i> highlighting the host-pathogen interactions. Few genes, involved in chickpea defense against Fusarium wilt which were not reported previously were unveiled in this study

Fusarium oxysporum mediates systems metabolic reprogramming of chickpea roots as revealed by a combination of proteomics and metabolomics

“…() and Gurjar et al . () have reported that Bet v1 protein is responsible for resistance towards pathogen. Similarly, β‐1,3‐glucanase is involved in plant defence response against pathogen (Shetty et al ., ; Ward et al ., ).…”

“…Many studies have been carried out to identify the molecular basis of Foc resistance or susceptibility in chickpea using various approaches including gene mapping, candidate gene identification, differential expression and biochemical analysis postfungal infection (Ashraf et al ., ; Giri et al ., ; Gowda et al ., ; Gupta et al ., , ; Gurjar et al ., ; Nimbalkar et al ., ). However, genomic scale dynamics of plant–fungus interaction is poorly understood.…”

Dynamics of Colonization and Expression of Pathogenicity Related Genes in Fusarium oxysporum f.sp. ciceri during Chickpea Vascular Wilt Disease Progression

“…Plant-pathogen interaction is complex and involves the expression of both, pathogen virulence genes as well as plant defense genes. Till date, various candidate genes with prime role in fungal pathogenesis have been identified [ 7 ]. These fungal pathogenicity genes are categorized based on formation of infection structures, cell wall degradation, toxin biosynthesis, signaling and proteins suppressing plant defense [ 8 – 10 ].…”

Systemic Resistance to Powdery Mildew in Brassica napus (AACC) and Raphanus alboglabra (RRCC) by Trichoderma harzianum TH12

“…The success of plant defense against pathogens depends on multiple events involved in the resistance. Moreover, these mechanisms of plant defense are governed by a range of genes singly or synergistically [ 23 ]. Some plants express resistance proteins that reveal the presence of specific elicitors, thereby leading to a strong defensive response, which is referred to as elicitor-triggered resistance [ 24 ].…”