2018
DOI: 10.1371/journal.pone.0205852
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Gene expression studies using a miniaturized thermal cycler system on board the International Space Station

Abstract: The distance and duration of human spaceflight missions is set to markedly increase over the coming decade as we prepare to send astronauts to Mars. However, the health impact of long-term exposure to cosmic radiation and microgravity is not fully understood. In order to identify the molecular mechanisms underpinning the effects of space travel on human health, we must develop the capacity to monitor changes in gene expression and DNA integrity in space. Here, we report successful implementation of three molec… Show more

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Cited by 17 publications
(12 citation statements)
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“…18 A series of DNA analysis technologies such as the polymerase chain reaction (PCR), quantitative PCR and DNA sequencing have already been demonstrated on the ISS. [19][20][21] These molecular biology | 161 RUBINFIEN Et al. technologies have contributed immensely to our understanding of the biological responses to microgravity in general. While all these efforts have been successful, they require specialized equipment and extensive molecular biology expertise to prepare, execute, and analyze the results.…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…18 A series of DNA analysis technologies such as the polymerase chain reaction (PCR), quantitative PCR and DNA sequencing have already been demonstrated on the ISS. [19][20][21] These molecular biology | 161 RUBINFIEN Et al. technologies have contributed immensely to our understanding of the biological responses to microgravity in general. While all these efforts have been successful, they require specialized equipment and extensive molecular biology expertise to prepare, execute, and analyze the results.…”
Section: Introductionmentioning
confidence: 99%
“…To study these biological changes that impact human health during and after spaceflight, the National Aeronautics and Space Administration (NASA) and other space agencies have established several platforms on the International Space Station (ISS) to enable advanced biological research . A series of DNA analysis technologies such as the polymerase chain reaction (PCR), quantitative PCR and DNA sequencing have already been demonstrated on the ISS . These molecular biology technologies have contributed immensely to our understanding of the biological responses to microgravity in general.…”
Section: Introductionmentioning
confidence: 99%
“…Moreover, thanks to its short life-cycle, the phenotyping could be done in C. elegans over multiple generations in space, as NASA has called for. The combination with sequencing technologies, which have recently been implemented on board the ISS, will allow analyses of space-induced genetic and epigenetic changes, in strict correlation to phenotypic changes, during missions on the ISS and beyond [105]. The peculiar features of C. elegans, including the possibility of maintaining it in hibernation and growing within microfluidic devices, would make it ideally suited as an innovative bio-dosimeter for deep space missions, including those conducted through micro-satellite platforms.…”
Section: Role Of Genetically Tractable Models In the Study Of Biologimentioning
confidence: 99%
“…com) as compared to $3000 USD for a conventional PCR thermocycler [28]. Several papers have documented the value of the miniPCR1 system as a portable and robust diagnostic tool [32][33][34][35][36]. We recently published a comparison of the performance of the miniPCR and a commercial thermal cycler for the identification of artificial Zika and Ebola genetic sequences.…”
Section: Introductionmentioning
confidence: 99%