Gene Identification and Characterization of Correlations for DEPs_DEGs Same Trend Responding to Salinity Adaptation inScylla paramamosain

Abstract

Scylla paramamosain is a commercially important species distributed along the coast of southern China and other Indo-Pacific countries. Sudden salinity drop exceeding the adjustment capability of S. paramamosain can result in damage or even mortality. In our previous study, we had analyzed the mechanism of adapting sudden drop in salinity from the level of transcriptomics and proteomics, respectively. This study performed a correlation analysis of RNA sequencing transcriptomics and iTRAQ proteomics in order to investigate the adaptation mechanisms to sudden salinity drop from 23‰ to 3‰. There were 3954 correlations and a total of 15 correlations for differentially expressed proteins (DEPs) and differentially expressed genes (DEGs) from proteomics and transcriptomics. The correlation between DEPs and DEGs was 0, and the Spearman correlation coefficient of the same trend correlation for DEPs and DEGs was the highest, reaching 0.9080. KEGG pathway enrichment correlation revealed that protein digestion and absorption (Ko04974), proximal tubule bicarbonate (Ko04964), and bile secretion (Ko04976) played important roles in Na+/H+ and Na+/K+ exchange. In addition, important genes related to osmoregulation, such as ion transport and carbonic anhydrase, were also detected in the correlation analysis for same trend DEPs_DEGs. In conclusion, the proteome and transcriptome correlation results from this study indicate that ion transport plays a critical role in the adaptation of S. paramamosain to sudden reduction in salinity.

Multi-omic approach provides insights into osmoregulation and osmoconformation of the crab Scylla paramamosain

“…We found a low correlation between expression levels of transcripts and proteins with only 31 differentially expressed genes in common at transcriptional and translational levels. The results are similar to findings from a previous study on salinity adaptation of mud crab 24 . Moreover, the poor correlation between transcript and protein abundance is also observed in other animal taxa 25 , 26 .…”

Effects of salinity on the ions important and sodium‐potassium ATPase in osmoregulation, cortisol, amino acids, digestive and immune enzymes in Scylla paramamosain during indoor overwintering

“…Salinity is an important factor affecting osmoregulation in S. paramamosain (Wang, Tang, Wei, Lu, Mu, et al, 2018; Wang, Wei, et al, 2019). Ion concentrations (K + , Cl ‐ and Na + ) and Na + /K + ‐ATPase activity are important indicators reflecting osmoregulation ability (Geng, Tian, et al, 2016; Geng, Tong, Jiang, & Xu, 2016; Nanba et al., 2012).…”

“…Salinity is one of the most variable water quality factors (Chand et al., 2015) that can directly affect osmotic regulation (Setiarto, Strussmann, Takashima, Watanabe, & Yokota, 2015; Wang et al., 2018a, b; Wang, Wei, et al, 2019), hormone levels (cortisol) (Kiilerich, Kristiansen, & Madsen, 2007), and the immune and digestive enzyme activities (Wang & Chen, 2006) of aquatic organisms. Due to a stress response, aquatic animals will experience from internal physiological dysfunction, and their immune defence ability will be reduced (Gheisvandi, Hajimoradloo, Ghorbani, & Hoseinifar, 2015), thereby affecting their growth and survival.…”

“…Salinity is also one of the important environmental factors influencing overwintering in S. paramamosain (Zhen & Shao, 2001). S. paramamosain is a euryhaline crab (Wang, Tang, Wei, Lu, Mu, et al, 2018; Wang et al., 2018a, b; Wang, Wei, et al, 2019); however, previous studies on the effects of salinity on osmotic pressure, plasma cortisol, digestive, immunity and amino acid of crabs have mainly focused on a sharp change (Lu, Zhuang, Feng, Zhang, & Wang, 2011). However, there are few studies on the effects of long‐term gradual changes in salinity on S. paramamosain .…”

Transcriptome analysis reveals hepatopancreatic genes and pathways associated with metabolism responding to water salinities during the overwintering of mud crab Scylla paramamosain

“…Scylla Paramamosain belongs to the family of Portunidae , and it is a eurythermic and euryhaline marine crab species (Wang et al, 2018a). It is characterized by fast growth, large size and strong adaptability, and it is considered an economically important crab species in China, with a high commodity value (Wang, Tang, et al, 2019; Wang, Wei, et al, 2019; Zhou, Li, Wang, Wang, et al, 2020). In recent years, the production rates of wild S. paramamosain have consistently declined due to the expansion of the breeding scale and the increase in market demand, and research on S. paramamosain aquaculture has gained increased attention.…”