Gene monitoring of surface-activated monocytes in circulating whole blood using duplex RT-PCR

Weber, Norbert; Wendel, Hans Peter; Ziemer, Gerhard

doi:10.1002/1097-4636(200107)56:1<1::aid-jbm1024>3.0.co;2-c

Cited by 15 publications

(11 citation statements)

References 31 publications

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“…dt Jn au • n dS = 0 Js (5) The fluid properties in the cell are determined as Pc = «pf + (\ -a)p" (6) Pc = <*Pf + (1 -< *K (7) where the subscript a indicates a property of the gas phase and a subscript / denotes a property of the liquid phase. The interface location within a given cell volume is tracked using a geometric reconstruction scheme [17].…”

Section: Numerical Setupmentioning

confidence: 70%

“…The loop can be rotated at various speeds for extended periods of time and requires relatively small samples of the blood or other working fluid under investigation. It has been employed in a variety of studies, including work on stents [2,3], heparin surface coatings [4], monocyte activation [5], and thrombus formation, lysis, and dissolution [6][7][8][9][10], to name a few.…”

Section: Introductionmentioning

confidence: 69%

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Numerical Investigation of Fluid Flow in a Chandler Loop

Touma

Sahin

Gaamangwe

et al. 2014

Journal of Biomechanical Engineering

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The Chandler loop is an artificial circulatory platform for in vitro hemodynamic experiments. In most experiments, the working fluid is subjected to a strain rate field via rotation of the Chandler loop, which, in turn, induces biochemical responses of the suspended cells. For low rotation rates, the strain rate field can be approximated using laminar flow in a straight tube. However, as the rotation rate increases, the effect of the tube curvature causes significant deviation from the laminar straight tube approximation. In this manuscript, we investigate the flow and associated strain rate field of an incompressible Newtonian fluid in a Chandler loop as a function of the governing nondimensional parameters. Analytical estimates of the strain rate from a perturbation solution for pressure driven steady flow in a curved tube suggest that the strain rate should increase with Dean number, which is proportional to the tangential velocity of the rotating tube, and the radius to radius of curvature ratio of the loop. Parametrically varying the rotation rate, tube geometry, and fill ratio of the loop show that strain rate can actually decrease with Dean number. We show that this is due to the nonlinear relationship between the tube rotation rate and height difference between the two menisci in the rotating tube, which provides the driving pressure gradient. An alternative Dean number is presented to naturally incorporate the fill ratio and collapse the numerical data. Using this modified Dean number, we propose an empirical formula for predicting the average fluid strain rate magnitude that is valid over a much wider parameter range than the more restrictive straight tube-based prediction.

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Section: Numerical Setupmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 69%

Numerical Investigation of Fluid Flow in a Chandler Loop

Touma

Sahin

Gaamangwe

et al. 2014

Journal of Biomechanical Engineering

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“…The most prominent inducible HSP is the 7,000 kDa protein, HSP70, and the expression of HSP70 mRNA is a valuable tool for estimation of biomaterial cytotoxicity [9][10][11][12][13][14]. In humans, at least eleven HSP70 genes have been previously identified [15].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Metal Materials on Heat Shock Protein 70B' Gene Expression

Okuda-Shimazaki¹,

Yamamoto²,

Kuroda³

et al. 2007

TOBIOTJ

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Abstract:To avoid the toxic effect of released nickel ions and compounds from conventional stainless steels, nickel-free austenitic stainless steels have been developed. We previously established a new manufacturing process to produce nickel-free austenitic stainless steel that involves nitrogen adsorption treatment. Although the cytocompatibility of nickelfree austenitic stainless steel produced using this method has been evaluated using two viability assay, molecular level analysis, such as gene expression analysis, has not been previously performed. In the present study, the cytotoxicity of our nickel-free austenitic stainless steel, as well as of commercially available metal materials, was evaluated by analysis of heat shock protein 70B' (HSP70B') gene expression as a stress response marker. Furthermore, to investigate the effect of metal materials on cytotoxicity, HSP70B' gene expression was quantified using human osteoblast-like SaOS-2 cells, human monocyte THP-1 cells and the mouse macrophage cell line J774A.1. We found no significant differences in HSP70B' expression among the various metal materials, including the nickel-free austenitic stainless steel, indicating that the nickel-free austenitic stainless steel produced using our nitrogen adsorption method has the same cytocompatibility as commercially available metal materials.

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“…The reverse transcription‐polymerase chain reaction (RT‐PCR) is one of the useful techniques for evaluating gene expression in cells. Gene expression in a small number of cells can be analyzed by the RT‐PCR because it is the most sensitive among available methods such as Northern blotting 13…”

Section: Introductionmentioning

confidence: 99%

“…The creation of HSPs is induced in cells by a variety of stress stimuli including heat shock, oxidative free radicals, toxic metal ions, and ultraviolet irradiation 17–19. A few studies of HSP gene expressions in cells exposed to biomaterials have been reported 13, 20, 21. Kishida and coworkers reported on the expression of HSP (HSP70, HSP90, and HSP47) mRNA in cells on various polymers 22.…”

Section: Introductionmentioning

confidence: 99%

Stress response of adherent cells on a polymer blend surface composed of a segmented polyurethane and MPC copolymers

Sawada

Iwasaki

Nakabayashi

et al. 2006

J Biomedical Materials Res

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To better understand the effect of 2-methacryloyloxyethyl phosphorylcholine (MPC) copolymer in improving the biocompatibility of segmented polyurethane (SPU), the expression of heat shock protein (HSP) mRNA in HeLa S3 cells adhered on SPU blended with MPC copolymers was measured. Conventionally, MPC copolymers (PMEH) were synthesized by changing the feed ratios of MPC and 2-ethylhexyl methacrylate. X-ray photoelectron spectroscopic analysis of the SPU/PMEH film indicated that the surface concentration of MPC units on the SPU/PMEH film increased with an increase in PMEH composition. HeLa S3 cells were cultured on SPU/PMEH films. The number of adherent cells on the SPU/PMEH films decreased with an increase in the concentration of PMEH. When the PMEH composition was greater than 0.5 wt %, cell adhesion and proliferation decreased markedly. Expressions of HSP27 and HSP47 mRNA were detected using the reverse transcription-polymerase chain reaction (RT-PCR). After incubation for 24 h, both the HSP mRNA expressions in the HeLa S3 cells showed no significant differences among all samples. In HeLa S3 cells that adhered to the SPU film for 48 h, the expressions of HSP27 and HSP47 mRNA increased significantly when compared with those incubated for 24 h. In contrast, the two kinds of mRNA expressions decreased in the HeLa S3 cells that adhered to the SPU/PMEH films for 48 h. From these results, we concluded that PMEH was quite important in suppressing the stress response of adherent HeLa S3 cells. Therefore, SPU/PMEH blend polymers are useful as implantable biomedical materials.

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Gene monitoring of surface-activated monocytes in circulating whole blood using duplex RT-PCR

Cited by 15 publications

References 31 publications

Numerical Investigation of Fluid Flow in a Chandler Loop

Numerical Investigation of Fluid Flow in a Chandler Loop

The Effect of Metal Materials on Heat Shock Protein 70B' Gene Expression

Stress response of adherent cells on a polymer blend surface composed of a segmented polyurethane and MPC copolymers

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