Gene transcription profiles of Saccharomyces cerevisiae after treatment with plant protection fungicides that inhibit ergosterol biosynthesis

Kagan, Isabelle A.; Michel, Albrecht; Prause, Anja; Scheffler, Brian E.; Pace, Pat; Duke, Stephen O.

doi:10.1016/j.pestbp.2005.02.002

Cited by 20 publications

(20 citation statements)

References 41 publications

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“…Although only one gene was analyzed, its expression pattern would seem to support that the sterol biosynthetic pathway in G. intraradices is in fact the target of fenpropimorph, while this does not seems to be the case for fenhexamid. This is in line with what has been proposed for yeast (Kagan, 2005) and it is also supported by the biochemical analyses.…”

Section: Discussionsupporting

confidence: 93%

“…These authors, using a microarray approach, showed no change in ERG25 expression levels in yeast cells treated with fenpropimorph or fenhexamid. Interestingly, the expression pattern of other ERG genes found by Kagan, 2005 supported the role of class II inhibitors (fenpropimorph) in affecting ergosterol biosynthesis, but strongly suggested that the class III inhibitor (fenhexamid) did not affect ergosterol biosynthesis in yeast. Although only one gene was analyzed, its expression pattern would seem to support that the sterol biosynthetic pathway in G. intraradices is in fact the target of fenpropimorph, while this does not seems to be the case for fenhexamid.…”

Section: Discussionmentioning

confidence: 84%

“…To our knowledge, only one paper, which was published by Kagan, 2005, refers to the use of the two fungicides used in this study -fenpropimorph and fenhexamid -in gene expression studies in S. cerevisiae. These authors, using a microarray approach, showed no change in ERG25 expression levels in yeast cells treated with fenpropimorph or fenhexamid.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Functional characterization of a C-4 sterol methyl oxidase from the endomycorrhizal fungus Glomus intraradices

Oger

Ghignone²,

Campagnac

et al. 2009

Fungal Genetics and Biology

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Functional characterization of a C-4 sterol methyl oxidase from the endomycorrhizal fungus Glomus intraradices

Oger

Ghignone²,

Campagnac

et al. 2009

Fungal Genetics and Biology

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“…Analysis of biosynthetic pathways, in addition to individual genes, may provide clearer hints to the mode of action of a toxicant. For example, although there were no profound effects of an array of ergosterol synthesis-inhibiting fungicides on any of the genes encoding enzymes of the ergosterol synthesis pathway in Saccharomyces cerevisiae , analysis of the pattern of effects on the entire pathway provided a signature that differentiated Class I and II ergosterol synthesis inhibitors from other fungicide modes of action (Kagan et al, 2005) (Fig. 2).…”

Section: Transcriptomicsmentioning

confidence: 99%

Omics Methods for Probing the Mode of Action of Natural and Synthetic Phytotoxins

2013

Self Cite

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For a little over a decade, omics methods (transcriptomics, proteomics, metabolomics, and physionomics) have been used to discover and probe the mode of action of both synthetic and natural phytotoxins. For mode of action discovery, the strategy for each of these approaches is to generate an omics profile for phytotoxins with known molecular targets and to compare this library of responses to the responses of compounds with unknown modes of action. Using more than one omics approach enhances the probability of success. Generally, compounds with the same mode of action generate similar responses with a particular omics method. Stress and detoxification responses to phytotoxins can be much clearer than effects directly related to the target site. Clues to new modes of action must be validated with in vitro enzyme effects or genetic approaches. Thus far, the only new phytotoxin target site discovered with omics approaches (metabolomics and physionomics) is that of cinmethylin and structurally related 5-benzyloxymethyl-1,2-isoxazolines. These omics approaches pointed to tyrosine amino-transferase as the target, which was verified by enzyme assays and genetic methods. In addition to being a useful tool of mode of action discovery, omics methods provide detailed information on genetic and biochemical impacts of phytotoxins. Such information can be useful in understanding the full impact of natural phytotoxins in both agricultural and natural ecosystems.

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“…Studies have reported that ingestion of pomegranate fruits has nutritional and health benefits, involving reduced oxidatlueive stress, and it has anticancer and antibacterial regulator effects (Santos et al 2012). Saccharomyces cerevisiae is important yeast that has been used for various studies (Kagan et al 2005;Comitini et al 2011). H 2 O 2 is a reactive oxygen species (ROS) in organism, being permanently produced intracellularly as a product of the metabolism in aerobic organisms and otherwise extracellularly during infection in specialist organisms (Lopes et al 2004;Cipak et al 2006;Folmer et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Anti-Oxidant effects of pomegranate juice on <i>Saccharomyces cerevisiae</i> cell growth

Aslan

Can

Boydak

2014

Afr. J. Trad. Compl. Alt. Med.

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Background: Pomegranate juice has a number of positive effects on both human and animal subjects. Material and methods: Four groups were used in this study. i: Control group, ii: H 2 O 2 group, iii: Pomegranate juice (PJ) group and iv: PJ + H 2 O 2 group. Following the sterilization method for pomegranate juice (10%) and H 2 O 2 (6% v/v), Saccharomyces cerevisiae cultures were added and the cultivation incubated at 35°C for 72 hours. Fatty acids and vitamin concentrations were measured using HPLC and GC and the total protein bands profile were determined by SDS-PAGE. Results: According to our results statistically significant differences have been determined among the study groups in terms of fatty acids and vitamin (p<0,05). Fatty acid synthesis, vitamin control and cell density increased in groups to which PJ was given in comparison with the control group (p<0,05). Pomegranate juice increased vitamins, fatty acids and total protein expression in Saccharomyces cerevisiae in comparison with the control. Conclusion: Pomegranate juice has a positive effect on fatty acid, vitamin and protein synthesis by Saccharomyces cerevisiae. Accordingly, we believe that it has significantly decreased oxidative damage thereby making a positive impact on yeast development.

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Gene transcription profiles of Saccharomyces cerevisiae after treatment with plant protection fungicides that inhibit ergosterol biosynthesis

Cited by 20 publications

References 41 publications

Functional characterization of a C-4 sterol methyl oxidase from the endomycorrhizal fungus Glomus intraradices

Functional characterization of a C-4 sterol methyl oxidase from the endomycorrhizal fungus Glomus intraradices

Omics Methods for Probing the Mode of Action of Natural and Synthetic Phytotoxins

Anti-Oxidant effects of pomegranate juice on <i>Saccharomyces cerevisiae</i> cell growth

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