Gene transfection of human mesenchymal stem cells with a nano‐hydroxyapatite–collagen scaffold containing DNA‐functionalized calcium phosphate nanoparticles

Abstract

This study aimed to fabricate a growth factor-releasing biodegradable scaffold for tissue regeneration. We prepared multishell calcium phosphate (CaP) nanoparticles functionalized with DNA, polyethyleneimine (PEI), protamine and octa-arginine (R8) and compared their respective transfection activity and cell viability measures using human mesenchymal stem cells. DNA-protamine complexes improved the transfection efficiency of CaP nanoparticles with the exception of those functionalized with R8. These complexes also greatly reduced the cytotoxicity of PEI. In addition, we also fabricated DNA-protamine-functionalized CaP nanoparticle-loaded nano-hydroxyapatite-collagen scaffolds and investigated their gene transfection efficiencies. These experiments showed that the scaffolds were associated with moderate hMSC cell viability and were capable of releasing the BMP-2 protein into hMSCs following gene transfection. In particular, the scaffold loaded with protamine-containing CaP nanoparticles showed the highest cell viability and transfection efficiency in hMSCs; thus, it might be suitable to serve as an efficient growth factor-releasing scaffold.

Entrapment of organic fluorophores in calcium phosphate nanoparticles with slow release

“…Future work should focus on other ways to control the release of organic substances from these nanoparticles and the possibility of extending their release. In terms of size and trapping efficiency, and release of an external agent, these results were comparable to the findings of similar work, but considerably more effective than those synthesized by other groups [36][37][38][39][40][41][42].…”

Calcium Phosphate Nanoparticles-Based Systems for RNAi Delivery: Applications in Bone Tissue Regeneration

“…Tenkumo et al fabricated multi-shell nanoparticles, by combining HA with functionalized pDNA, which was subsequently loaded in a collagen scaffold for periodontal tissue repair. These functionalized collagen scaffolds exhibited a higher transfection efficacy compared to Lipofectamine ® 2000, a widely used commercial available transfection agent [146].…”

Structural and Luminescent Properties of Europium-Doped and Undoped Hydroxyapatite Powders Sintered by Spark Plasma

“…Therefore, HA is widely used as a biomaterial in orthopaedic and dental applications; moreover, nanosized hydroxyapatite with excellent biocompatibility and thermal stability also has its possible applications in bioimaging and nanomedicine [3]. Recently, HA has been proposed to be useful in applications such as gene transfection [4,5], hyperthermia therapy [6], drugs delivery [7,8] and molecular imaging [9]. For these purposes, it is necessary to functionalise the hydroxyapatite nanoparticles by incorporating organic dyes, quantum dots, and rare earth elements [10,11].…”