General Methods for the Extraction, Purification, and Measurement of Steroids by Chromatography and Mass Spectrometry

Makin, H. L. J.; Honour, John W.; Shackleton, Cedric; Griffiths, William J.

doi:10.1023/b135931_3

Cited by 26 publications

(23 citation statements)

References 581 publications

(517 reference statements)

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“…The sterically hindered hydroxyl groups of side chain of UK1 and UK2 could prevent their derivatization by the alkylating reagent (TMS). [21] Looking at UK3 spectrum (Fig. 3A) in the region at lower masses, it was very similar to that of UK1 suggesting that A-B-C-D ring structure follows the same fragmentation pattern.…”

Section: Resultsmentioning

confidence: 61%

“…B is reported the UK2 spectrum in which, although showing a lower intensity of diagnostic ions, the presence of fragment ions at m / z 415 and 430 suggest that UK2 could be identified as 24‐hydroxycholest‐4‐en‐3‐one‐26‐oic acid. The sterically hindered hydroxyl groups of side chain of UK1 and UK2 could prevent their derivatization by the alkylating reagent (TMS) …”

Section: Resultsmentioning

confidence: 99%

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Sterol and steroid catabolites from cholesterol produced by the psychrophile Pseudoalteromonas haloplanktis

et al. 2014

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Pseudoalteromonas haloplanktis, a psychrotrophilic marine bacterium of biotechnological interest, shows anti-biofilm properties and is particularly relevant for cold storage of vacuum packed seafood. We focused our interest on the activation of cholesterol metabolism in this bacterium as the presence in its genome of a putative 3-ketosteroid-Δ(1) -dehydrogenase. This study reports GC-MS and LC-MS/MS profiles of sterols/steroids and their derivatives found in cell extracts of P. haloplanktis grown in a medium with a low content of cholesterol. Here, for the first time, we suggest that P. haloplanktis produces some intermediates of cholesterol catabolism, putatively identified as 24-hydroxycholest-1,4-dien-3-one-26-oic acid, chol-1,4-dien-3-one-24-oic acid, 26-hydroxycholest-4-en-3-one, and pregn-4-en-3-one-20-carboxylic acid, a finding already reported in other microorganisms. The presence of these compounds, also considered steroid precursors, produced by P. haloplanktis in vacuum packed seafood could be of interest for healthy of consumers, as well as, for biotechnological applications in pharmaceutical industry.

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Section: Resultsmentioning

confidence: 61%

Section: Resultsmentioning

confidence: 99%

Sterol and steroid catabolites from cholesterol produced by the psychrophile Pseudoalteromonas haloplanktis

et al. 2014

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“…It is still widely used for analysis of bile acids [7], steroids [8], cholesterol and its precursors [9] and oxysterols [10]. Many of the current methods are based on classical protocols developed in the laboratories of Sjövall [3], [6], Shackleton [11] and Björkhem [12], [13]. A pre-requisite for GC-MS analysis of sterols is derivatisation to enhance volatility and stability.…”

Section: Technologymentioning

confidence: 99%

Sterolomics in biology, biochemistry, medicine

Griffiths

2019

TrAC Trends in Analytical Chemistry

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In mammalian systems “sterolomics” can be regarded as the quantitative or semi-quantitative profiling of all metabolites derived from cholesterol and its cyclic precursors. The system can be further complicated by metabolites derived from ingested phytosterols or pharmaceuticals, but this is beyond the scope of this article. “Sterolomics” can be performed on either an unbiased global format, or more usually, exploiting a targeted format. Here we discuss the different mass spectrometry-based analytical techniques used in “sterolomics” giving specific examples in the context of neurodegenerative disease and for the diagnosis of inborn errors of metabolism. We pay particular attention to the profiling of cholesterol metabolites in the bile acid biosynthesis pathways, although the analytical techniques discussed are also appropriate for analysis of hormonal steroids.

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“…According to statistics, there are more than 150 steroids currently used for clinical treatment [1] . Terpenoids generally contain a steroidal nucleus, which is the reason for its special structure [2] .In recent years, due to the continuous development of medicine, the demand for steroids has surged [3] , natural extraction methods have been unable to meet market demand, and artificial synthesis has become new access to steroids. Androstenedione (AD) is an essential intermediate for the synthesis of most steroids in this context [4] .…”

Section: Introductionmentioning

confidence: 99%

Study on optimum technological conditions for producing androstenedione by microbial method

ShI

Yang

et al. 2019

E3S Web Conf.

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As an indispensable intermediate, androstenedione is widely used in drug manufacturing, especially steroidal drugs. However, the chemical manufacturing process of androstenedione is generally complicated and difficult, and it will cause serious environmental pollution in the production process. The biological method for the production of androstenedione has a very promising development prospect, because it is more economical and environmentally friendly than chemical methods. In order to better produce androstenedione on a large scale, the imbalance between supply and demand can be solved. In this study, the biaqueous phase system was used to increase the substrate concentration, and the method of transforming plant sterol by mycobacterium was used to produce androstenedione. The optimal conditions for the production of androstenedione by microbial assay were determined by orthogonal test: the aqueous two-phase system was water/ sunflower oil, the temperature was 30 °C, the initial pH was 6.5, the substrate concentration was 0.4 g/L, the rotation speed was 250 rpm, and the inoculation was carried out. The amount was 14.83%, the organic ratio was 20.65%, and the liquid loading was 150/500 mL. The preliminary production of androstenedione by microbial method has found suitable process conditions and provided data and theoretical support for its large-scale production.

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General Methods for the Extraction, Purification, and Measurement of Steroids by Chromatography and Mass Spectrometry

Cited by 26 publications

References 581 publications

Sterol and steroid catabolites from cholesterol produced by the psychrophile Pseudoalteromonas haloplanktis

Sterol and steroid catabolites from cholesterol produced by the psychrophile Pseudoalteromonas haloplanktis

Sterolomics in biology, biochemistry, medicine

Study on optimum technological conditions for producing androstenedione by microbial method

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