Single-step tightening is simple and fast compared to multi-step tightening, and it is therefore frequently used during the tightening of bolts in flange structures. However, single-step tightening to maintain uniformity is more difficult to achieve. To address this problem, existing methods such as the Elastic Interaction Coefficient Method (EICM) and Tetraparametric Assembly Method (TAM) have been investigated for load uniformity in single-step tightening. In order to improve the computational efficiency and accuracy of the traditional methods, a new single-step tightening method is proposed in this paper. This method can realize the design of the initial preload force simply by measuring the change in bolt load in a specific sequence. It is verified by numerical simulation that this method can realize the uniform distribution of bolt load. In addition, this paper will provide suggestions for the optimal tightening sequence for the single-step tightening method.