Generation and application of chicken egg-yolk antibodies

Tini, Mauro; Jewell, Ursula R.; Camenisch, Gieri; Chilov, Dmitri; Gassmann, Max

doi:10.1016/s1095-6433(01)00508-6

Cited by 149 publications

(82 citation statements)

References 21 publications

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“…As concentrações de proteína obtidas da purificação de IgG do soro e das gemas dos ovos de codorna apresentaram coerência com os resultados encontrados em estudos anteriores (Otani et al, 1993;De Meulenaer e Huyghebaert, 2001;Tini et al, 2002) para purificação de IgG de galinha.…”

Section: Tabela 1 Concentração De Proteína Das Amostras De Igg Purifunclassified

“…A IgG é a imunoglobulina encontrada em maior concentração no soro das aves, pode passar para o ovo, conferindo imunidade passiva para o pintinho recém-eclodido (Rosenberg, 1996;Tini et al, 2002). A gema de ovo pode ser usada em testes sorológicos, porém, devido à grande quantidade de lipídeos presentes, os anticorpos precisam ser purificados (Tini et al, 2002).…”

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“…A gema de ovo pode ser usada em testes sorológicos, porém, devido à grande quantidade de lipídeos presentes, os anticorpos precisam ser purificados (Tini et al, 2002). Os testes sorológicos convencionais utilizados para codornas (Coturnix coturnix japonica) têm sido baseados naqueles padronizados para galinhas (Gallus gallus formadomestica).…”

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Purificação de IgG de codorna a partir de soro e gema de ovos

Oliveira¹,

Resende²,

Flatschart³

et al. 2010

Arq. Bras. Med. Vet. Zootec.

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Section: Tabela 1 Concentração De Proteína Das Amostras De Igg Purifunclassified

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Purificação de IgG de codorna a partir de soro e gema de ovos

Oliveira¹,

Resende²,

Flatschart³

et al. 2010

Arq. Bras. Med. Vet. Zootec.

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“…The primary antibody was chicken a-SNFT antiserum (#12), generated by immunizing chickens with purified GST-SNFT and isolated from eggs as previously described (Tini et al, 2002), except that no affinity purification was used. a-SNFT #12 was diluted 1 : 4000 in 5% milk/TBST, and the secondary antibody was rabbit a-chicken-HRP (Sigma) diluted 1 : 10 000 in 5% milk/TBST.…”

Section: Western Blotsmentioning

confidence: 99%

Transcriptional repression of MMP-1 by p21SNFT and reduced in vitro invasiveness of hepatocarcinoma cells

2004

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p21 SNFT (21 kDa small nuclear factor isolated from T cells) is a human basic leucine zipper transcription factor that can repress AP-1-mediated transcription. We show here that overexpression of p21 SNFT in HepG2 cells leads to repression of matrix metalloproteinase-1 by 70-80%. p21 SNFT interacted with Jun at the matrix metalloproteinase-1 promoter À88 Ets/AP-1 enhancer element, where Jun is known to activate transcription via interaction with Fos and Ets proteins. When p21 SNFT /Jun dimers bound the element in the presence of Ets, DNA was protected differently than when Fos was paired with Jun. The data suggest a difference in overall conformation between p21 SNFT -containing and Fos-containing complexes that may be involved in the repression of matrix metalloproteinase-1 by p21 SNFT . Overexpression of p21 SNFT led to a reduction in invasiveness of HepG2 cells through type I collagen and reconstituted basement membrane, an effect similar to that obtained via direct immunodepletion of matrix metalloproteinase-1. The results indicate that the mechanism of repression of matrix metalloproteinase-1 by p21 SNFT may be exploited in inhibiting pathological matrix remodeling during cancer progression in vivo.

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“…A large amount of IgY (100-200 mg/egg) can be extracted at a low cost from the egg yolks of hens immunized via a simple procedure (11). Many IgY-based applications are currently under development (12)(13)(14)(15). This study focused on its lower reactivity with protein A since IgY lacks a structurally different Fc region (16).…”

Section: Introductionmentioning

confidence: 99%

Detection of Methicillin-Resistant Staphylococcus aureus Using a Specific Anti-PBP2a Chicken IgY Antibody

et al. 2013

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SUMMARY:Immunodetection of methicillin-resistant Staphylococcus aureus (MRSA) by conventional methods employing mammalian immunoglobulins has unknown detection limits, and often yields false-positive results because of the presence of S. aureus protein A, which binds the Fc region of mammalian IgG. In this study, a new PBP2a-specific chicken IgY antibody was developed in inbred and conventional chickens, and used for the detection of MRSA using whole cell lysate samples. Our results showed that this chicken IgY antibody minimized the side effects of protein A. Moreover, enzymelinked immunosorbent assay and immunochromatography systems were used with a monoclonal and polyclonal anti-PBP2a IgY antibody, clearly differentiating MRSA from methicillin-sensitive S. aureus and other methicillin-sensitive Staphylococcus spp. The detection limit of the immunochromatography was 10 8 colony-forming units; therefore, 1 colony on an agar plate was adequate to distinguish MRSA from non-MRSA. The specificity and sensitivity of this assay were almost similar to that of a commercially available latex agglutination test; however, the procedure used in this study was less complicated. The entire detection procedure, including sample preparation, takes only 20 min and does not require special equipment. Therefore, the use of this IgY antibody as a new tool for the detection of MRSA is highly recommended.

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Generation and application of chicken egg-yolk antibodies

Cited by 149 publications

References 21 publications

Purificação de IgG de codorna a partir de soro e gema de ovos

Purificação de IgG de codorna a partir de soro e gema de ovos

Transcriptional repression of MMP-1 by p21SNFT and reduced in vitro invasiveness of hepatocarcinoma cells

Detection of Methicillin-Resistant Staphylococcus aureus Using a Specific Anti-PBP2a Chicken IgY Antibody

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