Generation and characterization of an estrogen receptor alpha‐iCre knock‐in mouse

Park, Chan Jin; Chen, Guanglin; Koo, Yongbum; Lin, Po Ching; Cacioppo, Joseph A.; Prohaska, Hailey; Ko, Che Myong

doi:10.1002/dvg.23084

Cited by 6 publications

(4 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…stm.sciencemag.org/cgi/content/full/13/603/eabf1383/DC1 Materials and Methods Figs. S1 to S7 Movies S1 to S4 Data file S1 Reference (73) View/request a protocol for this paper from Bio-protocol.…”

Section: Supplementary Materialsmentioning

confidence: 99%

A small-molecule activator of the unfolded protein response eradicates human breast tumors in mice

et al. 2021

View full text Add to dashboard Cite

Metastatic estrogen receptor α (ERα)–positive breast cancer is presently incurable. Seeking to target these drug-resistant cancers, we report the discovery of a compound, called ErSO, that activates the anticipatory unfolded protein response (a-UPR) and induces rapid and selective necrosis of ERα-positive breast cancer cell lines in vitro. We then tested ErSO in vivo in several preclinical orthotopic and metastasis mouse models carrying different xenografts of human breast cancer lines or patient-derived breast tumors. In multiple orthotopic models, ErSO treatment given either orally or intraperitoneally for 14 to 21 days induced tumor regression without recurrence. In a cell line tail vein metastasis model, ErSO was also effective at inducing regression of most lung, bone, and liver metastases. ErSO treatment induced almost complete regression of brain metastases in mice carrying intracranial human breast cancer cell line xenografts. Tumors that did not undergo complete regression and regrew remained sensitive to retreatment with ErSO. ErSO was well tolerated in mice, rats, and dogs at doses above those needed for therapeutic responses and had little or no effect on normal ERα-expressing murine tissues. ErSO mediated its anticancer effects through activation of the a-UPR, suggesting that activation of a tumor protective pathway could induce tumor regression.

show abstract

Section: Supplementary Materialsmentioning

confidence: 99%

A small-molecule activator of the unfolded protein response eradicates human breast tumors in mice

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Their expression and transcriptional activity in the course of embryo development is less studied (Brandenberger et al 1997; Lemmen et al 1999). In addition to selective KO mutation of ERα, ERβ, or the aromatase gene ( Cyp19a1 , encoding the enzyme responsible for testosterone-derived oestrogen synthesis) that highlighted the relevance of these receptors and their cognate ligands for the development of sexual organs and sexual behaviour (Kudwa, et al 2006), the elucidation of the ER distribution and activity during implantation and embryogenesis in non-reproductive tissues is circumscribed to only a few studies (Bondesson, et al 2015; Mogi, et al 2015; Park, et al 2017). However, preclinical and clinical observations in subjects carrying mutations that impair ER signalling showed deviations from the proper development of the cardiovascular system (Del Principe, et al 2015; Tait et al 2015), innate immune and neuro-immune communications (Zoller and Kersh 2006), pancreatic and gastric activity (Campbell-Thompson, et al 2001; Maniu, et al 2016), and liver functions (Barros and Gustafsson 2011; Bryzgalova, et al 2006; Foryst-Ludwig, et al 2008), as well as adipose (Barros and Gustafsson 2011; Lapid et al 2014), lung (Carey, et al 2007; Patrone, et al 2003; Thuresson-Klein et al 1985), kidney (Kummer, et al 2011; Lane 2008), and epidermal tissues with the muscle-skeletal apparatus (Brandenberger et al 1997; Hanley et al 1996; Lemmen et al 1999; Markiewicz, et al 2013; Takeyama et al 2001; Ueberschlag-Pitiot, et al 2017; Walker and Korach 2004).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional activity of oestrogen receptors in the course of embryo development

Torre

Rando

Meda

et al. 2018

Journal of Endocrinology

View full text Add to dashboard Cite

Oestrogens are well-known proliferation and differentiation factors that play an essential role in the correct development of sex-related organs and behaviour in mammals. With the use of the ERE-Luc reporter mouse model, we show herein that throughout mouse development, oestrogen receptors (ERs) are active starting from day 12 post conception. Most interestingly, we show that prenatal luciferase expression in each organ is proportionally different in relation to the germ layer of the origin. The luciferase content is highest in ectoderm-derived organs (such as brain and skin) and is lowest in endoderm-derived organs (such as liver, lung, thymus and intestine). Consistent with the testosterone surge occurring in male mice at the end of pregnancy, in the first 2 days after birth, we observed a significant increase in the luciferase content in several organs, including the liver, bone, gonads and hindbrain. The results of the present study show a widespread transcriptional activity of ERs in developing embryos, pointing to the potential contribution of these receptors in the development of non-reproductive as well as reproductive organs. Consequently, the findings reported here might be relevant in explaining the significant differences in male and female physiopathology reported by a growing number of studies and may underline the necessity for more systematic analyses aimed at the identification of the prenatal effects of drugs interfering with ER signalling, such as aromatase inhibitors or endocrine disrupter chemicals.

show abstract

“…This reporter mouse model supports longitudinal and functional monitoring of T cells in vivo. Park et al reported estrogen receptor alpha‐iCre mouse line which express codon‐improved Cre (iCre) driven by the Esr1 promoter. They further crossed ROSA26‐LacZ reporter mouse strain with Esr1‐iCre mouse line to characterize the function of lineage‐tracing Esr1‐expressing cells.…”

Section: Rosa26 Reporter Animal Modelsmentioning

confidence: 99%

Overview of the reporter genes and reporter mouse models

Chen

Peng

et al. 2018

Anim Models and Exp Med

View full text Add to dashboard Cite

Reporter genes are widely applied in biotechnology and biomedical research owning to their easy observation and lack of toxicity. Taking advantage of the reporter genes in conjunction with imaging technologies, a large number of reporter mouse models have been generated. Reporter mouse models provide systems that enable the studies of live cell imaging, cell lineage tracing, immunological research and cancers etc. in vivo. In this review, we describe the types of different reporter genes and reporter mouse models including, random reporter strains, Cre reporter strains and ROSA 26 reporter strains. Collectively, these reporter mouse models have broadened scientific inquires and provided potential strategies for generation of novel reporter animal models with enhanced capabilities.

show abstract

Generation and characterization of an estrogen receptor alpha‐iCre knock‐in mouse

Cited by 6 publications

References 56 publications

A small-molecule activator of the unfolded protein response eradicates human breast tumors in mice

A small-molecule activator of the unfolded protein response eradicates human breast tumors in mice

Transcriptional activity of oestrogen receptors in the course of embryo development

Overview of the reporter genes and reporter mouse models

Contact Info

Product

Resources

About