Generation and characterization of <scp>PDGFR</scp>α‐<scp>GFPC</scp>re<scp>ER</scp><sup>t2</sup> knock‐In mouse line

Miwa, Hiroyuki; Era, Takumi

doi:10.1002/dvg.22853

Cited by 26 publications

(21 citation statements)

References 33 publications

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“…60 In the heart, PDGFRα is robustly expressed in fibroblasts, with relatively limited expression in other cell types, including smooth muscle cells 61 and possible expression in cardiac progenitor cells; 62 it has been utilized for the development of an inducible Cre system, the PDGFRα-GFPCreERT2 knock-in mouse line. 63 This will likely prove a useful tool in characterizing CFs and their role in fibrosis. PDGFRβ has also been used as a marker of fibroblasts.…”

Section: Cardiac Fibroblast Markers and Limitationsmentioning

confidence: 99%

Cardiac Fibrosis

Travers

Kamal

Robbins

et al. 2016

Circulation Research

1,250

662

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Myocardial fibrosis is a significant global health problem associated with nearly all forms of heart disease. Cardiac fibroblasts comprise an essential cell type in the heart that is responsible for the homeostasis of the extracellular matrix; however upon injury, these cells transform to a myofibroblast phenotype and contribute to cardiac fibrosis. This remodeling involves pathological changes that include chamber dilation, cardiomyocyte hypertrophy and apoptosis, and ultimately leads to the progression to heart failure. Despite the critical importance of fibrosis in cardiovascular disease, our limited understanding of this cell population impedes the development of potential therapies that effectively target this cell type and its pathological contribution to disease progression. This review summarizes current knowledge regarding the origins and roles of fibroblasts, mediators and signaling pathways known to influence fibroblast function after myocardial injury, as well as novel therapeutic strategies under investigation to attenuate cardiac fibrosis.

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Section: Cardiac Fibroblast Markers and Limitationsmentioning

confidence: 99%

Cardiac Fibrosis

Travers

Kamal

Robbins

et al. 2016

Circulation Research

1,250

662

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“…Furthermore, Platelet-derived growth factor receptor α (Pdgfrα) and platelet-derived growth factor receptor β (Pdgfrβ) belong to the family of receptor tyrosine kinases (42). Pdgfrα and Pdgfrβ play important roles in forming the vasculature during embryonic development and are expressed in cardiovascular and mesenchymal progenitor cells (43,44). Pdgfrβ regulates the development of vascular smooth muscle cells from epicardium-derived cells (45).…”

Section: Cd90 (Thy1)mentioning

confidence: 99%

Cardiac fibroblasts: more than mechanical support

et al. 2017

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Fibroblasts are cells with a structural function, synthesizing components of the extracellular matrix. They are accordingly associated with various forms of connective tissue. During cardiac development fibroblasts originate from different sources. Most derive from the epicardium, some derive from the endocardium, and a small population derives from the neural crest. Cardiac fibroblasts have important functions during development, homeostasis, and disease. However, since fibroblasts are a very heterogeneous cell population no truly specific markers exist. Therefore, studying them in detail is difficult. Nevertheless, several lineage tracing models have been widely used. In this review, we describe the developmental origins of cardiac fibroblasts, comment on fibroblast markers and related lineage tracing approaches, and discuss the cardiac cell composition, which has recently been revised, especially in terms of non-myocyte cells.

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“…Cre recombinase is used to delete a segment of DNA flanked by LoxP sites (flox). The ERT2 system is used to activate Cre activity by tamoxifen treatment[10,11]. …”

Section: Introductionmentioning

confidence: 99%

Construction of Gpm6a/Reelin^GFPCreERT2 by BAC recombination using a specific gene in hepatic mesothelial or stellate cells

Lou

Shi

Ren

et al. 2017

WJG

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AIMTo prepare a Gpm6a/ReelinGFPCreERT2 construct with a rapid and reliable strategy using a bacterial artificial chromosome (BAC).METHODSGpm6a and Reelin BACs were purified and transformed into SW102 E. coli by electroporation. The GFPCreERT2 fragment was prepared from a shuttle vector and transformed into SW102 E. coli carrying a BAC. Homologous recombination was induced in SW102 E. coli. Recombinant clones were screened and confirmed by PCR and restriction enzyme digestion. Recombinant clones were transformed into SW102 E. coli to remove the kanamycin unit.RESULTSA complete BAC was successfully transformed into SW102 E. coli by electroporation because BAC purified from SW102 E. coli showed the same pattern as the original BAC with BamHI digestion. The GFPCreERT2 fragment was deemed to have been prepared successfully because we obtained the same size fragment as expected. Homologous recombination was induced, and GFPCreERT2 was deemed to have been inserted into the correct site of the BAC because we found the band change was the same as the expected pattern after restriction enzyme digestion. The kanamycin unit was deemed to have been removed successfully because we obtained different sizes of bands that were consistent with the results expected by PCR with different primers.CONCLUSIONThe construct of Gpm6aGFPCreERT2 or ReelinGFPCreERT2 was prepared successfully, which will establish a foundation for tracing the hepatic stellate cell lineage and studying its function.

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Generation and characterization of PDGFRα‐GFPCreER^t2 knock‐In mouse line

Cited by 26 publications

References 33 publications

Cardiac Fibrosis

Cardiac Fibrosis

Cardiac fibroblasts: more than mechanical support

Construction of Gpm6a/Reelin^GFPCreERT2 by BAC recombination using a specific gene in hepatic mesothelial or stellate cells

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Generation and characterization of PDGFRα‐GFPCreERt2 knock‐In mouse line

Cited by 26 publications

References 33 publications

Cardiac Fibrosis

Cardiac Fibrosis

Cardiac fibroblasts: more than mechanical support

Construction of Gpm6a/ReelinGFPCreERT2 by BAC recombination using a specific gene in hepatic mesothelial or stellate cells

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Product

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Generation and characterization of PDGFRα‐GFPCreER^t2 knock‐In mouse line

Construction of Gpm6a/Reelin^GFPCreERT2 by BAC recombination using a specific gene in hepatic mesothelial or stellate cells