Generation of a co-culture cell micropattern model to simulate lung cancer bone metastasis for anti-cancer drug evaluation

Zhong, Huixiang; Liu, Xuan; Wang, Dandan; Zhou, Jianhua; Li, Yan; Jiang, Qing

doi:10.1039/c7ra01868a

Cited by 14 publications

(18 citation statements)

References 40 publications

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“…On the aligned samples, cells obtained an elongated morphology, aligned to the micropattern and mainly located on the microstrips (Figure S2, Supporting Information; Figure a). The empty space between the microstrips may facilitate cell migration and proliferation . A significantly greater number of cells was measured after 7 d of seeding (Figure S3, Supporting Information), indicating the peptide/silica micropattern did not compromise hMSC proliferation.…”

Section: Resultsmentioning

confidence: 99%

“…Aligned micropattern cues are crucial for cell proliferation, migration, and differentiation, and also result in the secretion and deposition of anistropic extracellular matrix (ECM) specific to tissue types . Our recent work on hMSC differentiation in silicified silk/R5 composites demonstrated that this composite material promoted osteogenic differentiation of hMSCs in an osteoinductive environment in vitro, as compared to the silk plain controls .…”

Section: Resultsmentioning

confidence: 99%

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Coding Cell Micropatterns Through Peptide Inkjet Printing for Arbitrary Biomineralized Architectures

Ling

Chen

et al. 2018

Adv Funct Materials

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Well-designed micropatterns present in native tissues and organs involve changes in extracellular matrix compositions, cell types and mechanical properties to reflect complex biological functions. However, the design and fabrication of these micropatterns in vitro to meet task-specific biomedical applications remains a challenge. A de novo design strategy to code and synthesize functional micropatterns is presented to engineer cell alignment through the integration of aqueous-peptide inkjet printing and site-specific biomineralization. The inkjet printing provides direct writing of macroscopic biosilica selective peptide-R5 patterns with micrometer-scale resolution on the surface of a biopolymer (silk) hydrogel. This is combined with in situ biomineralization of the R5 peptide for site-specific growth of silica nanoparticles on the micropatterns, avoiding the use of harsh chemicals or complex processing. The functional micropatterned systems are used to align human mesenchymal stem cells and bovine serum albumin. This combination of peptide printing and site-specific biomineralization provides a new route for developing cost-effective micropatterns, with implications for broader materials designs.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Coding Cell Micropatterns Through Peptide Inkjet Printing for Arbitrary Biomineralized Architectures

Ling

Chen

et al. 2018

Adv Funct Materials

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“…The micropattern platform creates multi-organ co-culture in a single compartment with different organ cells spatially separated using cell micropatterning techniques (Figure 2A (c)). [66] Different types of cells are often selectively attached to or removed from 2D culture substrate with regional (patterned) surface modification that tunes cell attachment, [67,68] or encapsulated in different hydrogels patterned in 3D configuration. [69] Different organ cell interactions are mainly mediated by diffusion through the overlying medium, and sometimes also by physical contact with neighboring cells.…”

Section: Advances In Multi-organ Integration Platformsmentioning

confidence: 99%

Multiorgan Microphysiological Systems for Drug Development: Strategies, Advances, and Challenges

Wang

Carmona

Hickman

et al. 2017

Adv Healthcare Materials

116

105

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Traditional cell culture and animal models utilized for preclinical drug screening have led to high attrition rates of drug candidates in clinical trials due to their low predictive power for human response. Alternative models using human cells to build in vitro biomimetics of the human body with physiologically relevant organ-organ interactions hold great potential to act as "human surrogates" and provide more accurate prediction of drug effects in humans. This review is a comprehensive investigation into the development of tissue-engineered human cell-based microscale multiorgan models, or multiorgan microphysiological systems for drug testing. The evolution from traditional models to macro- and microscale multiorgan systems is discussed in regards to the rationale for recent global efforts in multiorgan microphysiological systems. Current advances in integrating cell culture and on-chip analytical technologies, as well as proof-of-concept applications for these multiorgan microsystems are discussed. Major challenges for the field, such as reproducibility and physiological relevance, are discussed with comparisons of the strengths and weaknesses of various systems to solve these challenges. Conclusions focus on the current development stage of multiorgan microphysiological systems and new trends in the field.

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“…Yet although such experiments are common on tissue and groups of cells embedded in synthetic matrices, they are rarely performed on individual cells in vitro. Batch processes that apply force to populations of cells, whether in 3D matrices or in monolayers [4]- [9], [12], [13], make it difficult to precisely control the loading conditions applied to each cell and to isolate the force response from other contributing factors. This isolation is crucial to investigating how mechanical deformation alone affects both suspended and adherent cells, which once understood can shed light on the role of more complicated interactions between cells and their surroundings when subjected to force.…”

Section: Introductionmentioning

confidence: 99%

Investigating Cellular Response to Impact With a Microfluidic MEMS Device

Patterson

Walker

Rodriguez-Mesa

et al. 2020

J. Microelectromech. Syst.

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Although high strain and strain-rate impacts to the human body have been the subject of substantial research at both the systemic and tissue levels, little is known about the celllevel ramifications of such assaults. This is largely due to the lack of high throughput, dynamic compression devices capable of simulating such traumatic loading conditions on individual cells. To fill this gap, we developed and characterized a high speed, high actuation force, magnetically driven MEMS chip to apply stress to biological cells at unprecedented strain (10% to 90%), strain rate (30,000 to 200,000 s −1), and throughput (12,000 cells/min). To demonstrate the capabilities of the µHammer, we applied biologically relevant strains and strain rates to human leukemic K562 cells and then monitored their viability for up to 8 days. We observed significantly repressed proliferation of the hit cells compared to both unperturbed and sham-hit control cells, accompanied by minimal cell death. This indicates success in applying cellular damage without compromising the overall viability of the population, allowing us to conclude that this device is well suited to study the subtle effects of impact on large populations of inherently heterogeneous cells.

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Generation of a co-culture cell micropattern model to simulate lung cancer bone metastasis for anti-cancer drug evaluation

Abstract: A549/OB co-culture micropattern was fabricated through μ-eraser strategy to mimic lung cancer bone metastasis for DOX efficacy evaluation.

Cited by 14 publications

References 40 publications

Coding Cell Micropatterns Through Peptide Inkjet Printing for Arbitrary Biomineralized Architectures

Coding Cell Micropatterns Through Peptide Inkjet Printing for Arbitrary Biomineralized Architectures

Multiorgan Microphysiological Systems for Drug Development: Strategies, Advances, and Challenges

Investigating Cellular Response to Impact With a Microfluidic MEMS Device

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