Generation of a Highly Biomimetic Organoid, Including Vasculature, Resembling the Native Immature Testis Tissue

Cham, Tat-Chuan; Ibtisham, Fahar; Fayaz, Mohammad Amin; Honaramooz, Ali

doi:10.3390/cells10071696

Cited by 30 publications

(38 citation statements)

References 81 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, bovine Leydig, Sertoli and peritubular myoid cells were isolated from testes and cultured separately, mixed at a concentration of 1 × 10 6 cells/mL, cultured for 7 days and then transferred to Matrigel for further 3D organization. This protocol differs from those commonly used in 3D testicular models, where testicular cells are obtained through enzymatic digestion and the remotion of connective tissue [ 6 , 8 , 30 , 32 , 34 , 48 ]. These cell isolation protocols may unintentionally provide other cell types to the testicular cell mix, including endothelial-like or uncharacterized cell types that may interfere with further experiments [ 8 , 30 , 31 , 32 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

“…This protocol differs from those commonly used in 3D testicular models, where testicular cells are obtained through enzymatic digestion and the remotion of connective tissue [ 6 , 8 , 30 , 32 , 34 , 48 ]. These cell isolation protocols may unintentionally provide other cell types to the testicular cell mix, including endothelial-like or uncharacterized cell types that may interfere with further experiments [ 8 , 30 , 31 , 32 , 49 ]. Following our testicular cell isolation method, we obtained cultures with populations of 21–37% of Leydig cells, 65–77% of Sertoli cells and 10–16% of peritubular myoid cells.…”

Section: Discussionmentioning

confidence: 99%

“…Organoids have generated great interest in recent years, since they are considered a useful tool to perform in vitro studies in different areas including toxicology, virology, oncology, microbiology and reproduction [ 3 , 4 , 5 , 6 ]. In particular, testicular organoids (TOs) have enabled the study of testicular development and have been used as a model for assaying the effects of different experimental factors and treatments [ 7 , 8 , 9 ]. In this regard, TOs may recreate conditions of the testicular niche, where spermatogenesis occurs through the self-renewing and differentiation of spermatogonial stem cells (SSCs) [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Generation and Characterization of Bovine Testicular Organoids Derived from Primary Somatic Cell Populations

Cortez

Leiva

Torres

et al. 2022

Animals

View full text Add to dashboard Cite

Organoids are 3D-culture systems composed of tissue-specific primary cells that self-organize and self-renew, creating structures similar to those of their tissue of origin. Testicular organoids (TOs) may recreate conditions of the testicular niche in domestic and wild cattle; however, no previous TO studies have been reported in the bovine species. Thus, in the present study, we sought to generate and characterize bovine TOs derived from primary testicular cell populations including Leydig, Sertoli and peritubular myoid cells. Testicular cells were isolated from bovine testes and cultured in ultra-low attachment (ULA) plates and Matrigel. TOs were cultured in media supplemented from day 3 with 100 ng/mL of BMP4 and 10 ng/mL of FGF2 and from day 7 with 15 ng/mL of GDNF. Testicular cells were able to generate TOs after 3 days of culture. The cells positive for STAR (Leydig) and COL1A (peritubular myoid) decreased (p < 0.05), whereas cells positive for WT1 (Sertoli) increased (p < 0.05) in TOs during a 28-day culture period. The levels of testosterone in media increased (p < 0.05) at day 28 of culture. Thus, testicular cells isolated from bovine testes were able to generate TOs under in vitro conditions. These bovine TOs have steroidogenic activity characterized by the production of testosterone.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Generation and Characterization of Bovine Testicular Organoids Derived from Primary Somatic Cell Populations

Cortez

Leiva

Torres

et al. 2022

Animals

View full text Add to dashboard Cite

show abstract

“…Testis-specific cell types such as germ cells, Sertoli cells, Leydig cells, and peritubular myoid cells, were identified in these organoids. More recently, porcine organoids with vascular structures was reported for the first time allowing the coexistence of various cell types in organoid culture system ( 70 ).…”

Section: Development Of Farm and Companion Animal Organoid Models: Hi...mentioning

confidence: 99%

“…In general, pigs are considered as superior animal models to mice due to their physiological and anatomical similarities to humans when considering biomedical and pharmaceutical studies ( 70 ). Therefore, studies on porcine testicular organoids possess wide application potentials for a variety of clinical and preclinical research including investigation of causes and treatment of male infertility and strategies to preserve fertility potentials in patients undertaking chemotherapy.…”

Section: Development Of Farm and Companion Animal Organoid Models: Hi...mentioning

confidence: 99%

Farm and Companion Animal Organoid Models in Translational Research: A Powerful Tool to Bridge the Gap Between Mice and Humans

Kawasaki

Goyama

Tachibana

et al. 2022

Front. Med. Technol.

View full text Add to dashboard Cite

Animal organoid models derived from farm and companion animals have great potential to contribute to human health as a One Health initiative, which recognize a close inter-relationship among humans, animals and their shared environment and adopt multi-and trans-disciplinary approaches to optimize health outcomes. With recent advances in organoid technology, studies on farm and companion animal organoids have gained more attention in various fields including veterinary medicine, translational medicine and biomedical research. Not only is this because three-dimensional organoids possess unique characteristics from traditional two-dimensional cell cultures including their self-organizing and self-renewing properties and high structural and functional similarities to the originating tissue, but also because relative to conventional genetically modified or artificially induced murine models, companion animal organoids can provide an excellent model for spontaneously occurring diseases which resemble human diseases. These features of companion animal organoids offer a paradigm-shifting approach in biomedical research and improve translatability of in vitro studies to subsequent in vivo studies with spontaneously diseased animals while reducing the use of conventional animal models prior to human clinical trials. Farm animal organoids also could play an important role in investigations of the pathophysiology of zoonotic and reproductive diseases by contributing to public health and improving agricultural production. Here, we discuss a brief history of organoids and the most recent updates on farm and companion animal organoids, followed by discussion on their potential in public health, food security, and comparative medicine as One Health initiatives. We highlight recent evolution in the culturing of organoids and their integration with organ-on-a-chip systems to overcome current limitations in in vitro studies. We envision multidisciplinary work integrating organoid culture and organ-on-a-chip technology can contribute to improving both human and animal health.

show abstract

Organoids of the male reproductive system: Challenges, opportunities, and their potential use in fertility research

Patrício

Santiago

Mano

et al. 2022

WIREs Mechanisms of Disease

View full text Add to dashboard Cite

Organoids are units of function of a given organ able to reproduce, in culture, a biological structure similar in architecture and function to its counterpart in vivo. Today, it is possible to develop an organoid from a fragment of tissue, a stem cell located in an adult organ, an embryonic stem cell, or an induced pluripotent stem cell. In the past decade, many organoids have been developed which mimic stomach, pancreas, liver and brain tissues, optic cups, among many others. Additionally, different male reproductive system organs have already been developed as organoids, including the prostate and testis. These 3D cultures may be of great importance for urological cancer research and have the potential to be used in fertility research for the study of spermatozoa production and maturation, germ cells–somatic cells interactions, and mechanisms of disease. They also provide an accurate preclinical pipeline for drug testing and discovery, as well as for the study of drug resistance. In this work, we revise the current knowledge on organoid technology and its use in healthcare and research, describe the male reproductive system organoids and other biomaterials already developed, and discuss their current application. Finally, we highlight the research gaps, challenges, and opportunities in the field and propose strategies to improve the use of organoids for the study of male infertility situations. This article is categorized under: Reproductive System Diseases > Stem Cells and Development Reproductive System Diseases > Biomedical Engineering

show abstract

Generation of a Highly Biomimetic Organoid, Including Vasculature, Resembling the Native Immature Testis Tissue

Cited by 30 publications

References 81 publications

Generation and Characterization of Bovine Testicular Organoids Derived from Primary Somatic Cell Populations

Generation and Characterization of Bovine Testicular Organoids Derived from Primary Somatic Cell Populations

Farm and Companion Animal Organoid Models in Translational Research: A Powerful Tool to Bridge the Gap Between Mice and Humans

Organoids of the male reproductive system: Challenges, opportunities, and their potential use in fertility research

Contact Info

Product

Resources

About