Generation of a proton motive force by the excretion of metal-phosphate in the polyphosphate-accumulating Acinetobacter johnsonii strain 210A.

Veen, Hendrik W van; Abee, Tjakko; Kortstee, G.J.J.; Pereira, Heloíza Vieira Rodrigues; Konings, Wn; Zehnder, Alexander J. B.

doi:10.1016/s0021-9258(18)43909-9

Cited by 66 publications

(12 citation statements)

References 37 publications

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“…Bacteria that accumulate large stores of phosphate under oxic conditions are under active investigation as biological agents to remove phosphorus from wastewaters (see Seviour for a review of this field). One of these phosphate-accumulating bacteria, Acinetobacter johnsonii 210A, was observed to uptake and then reduce the orthophosphate levels to micromolar concentrations by generating polyphosphate. , Mitochondria have detectable polyphosphate stores , that may act as orthophosphate reservoirs to meet the orthophosphate demand.…”

Section: Polyphosphate Biochemistrymentioning

confidence: 99%

“…Acinotobacter johnsonii produces a neutral MeHPO 4 complex as a biproduct of energy production. This complex can be excreted with H + , generating a proton motive force . Phosphate transport in prokaryotes, including proposed roles of polyphosphates, have been reviewed by van Veen .…”

Section: Polyphosphate Biochemistrymentioning

confidence: 99%

“…One of these phosphateaccumulating bacteria, Acinetobacter johnsonii 210A, was observed to uptake and then reduce the orthophosphate levels tomicromolarconcentrationsbygeneratingpolyphosphate. 218,219 Mitochondria have detectable polyphosphate stores 101,220 that may act as orthophosphate reservoirs to meet the orthophosphate demand.…”

Section: Phosphate Storagementioning

confidence: 99%

“…This complex can be excreted with H + , generating a proton motive force. 219 Phosphate transport in prokaryotes, including proposed roles of polyphosphates, have been reviewed by van Veen. 244 The metabolism of polyphosphates by these polyphosphate-accumulating bacteria in activated sludge was theorized to have ecological significance in the phosphorus dynamics of freshwater ecosystems as well.…”

Section: Energy Sourcementioning

confidence: 99%

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Relationships between Polyphosphate Chemistry, Biochemistry and Apatite Biomineralization

2008

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Section: Polyphosphate Biochemistrymentioning

confidence: 99%

Section: Polyphosphate Biochemistrymentioning

confidence: 99%

Section: Phosphate Storagementioning

confidence: 99%

Section: Energy Sourcementioning

confidence: 99%

See 2 more Smart Citations

Relationships between Polyphosphate Chemistry, Biochemistry and Apatite Biomineralization

2008

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“…During the anaerobic phase of EBPR, the electrogenic release of PP occurs in conjunction with the production of protons to generate a proton motive force that energises the uptake of acetate to form poly3-hydroxybutyrate (PHB) (Van Veen et al, 1994). Noting that PHB is more of a reduced compound than acetate, it has been proposed that the reducing power for its synthesis is mainly from intracellularly stored glycogen (Mino et al, 1994;Smolders et al 1995;Pereira et al, 1996) and also possibly from nicotinamide-adenine dinucleotide (NADH 2 ) formed by the oxidation of acetate to carbon dioxide (CO 2 ) (Comeau et al, 1985;Wentzel, 1988).…”

Section: Pao Behaviour In Phosphorus Removal Unit Processesmentioning

confidence: 99%

Plantwide modelling – anaerobic digestion of waste sludge from parent nutrient (N and P) removal systems

Ikumi

Ekama

2019

WSA

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Wastewater treatment plant (WWTP) mathematical models are based on the behavioural patterns of microorganisms involved in the treatment process. These microorganisms are assumed incapable of thinking or planning but simply act according to the capabilities afforded to them by their surrounding conditions – hence different microorganisms pre-dominate different WWTP zones according to how well the conditions suit them. When waste activated sludge (WAS) from biological nutrient removal (BNR) activated sludge (AS) systems, containing phosphorus-accumulating organisms (PAOs), is fed to an anaerobic digester, there is a release of high quantities of metals, phosphorus (P) and nitrogen (N). The manner in which we model the release of these metals and nutrients significantly affects the accuracy of predicted anaerobic digestion (AD) outcomes. Previous studies of PAOs show that in the anaerobic zone of the AS system, they can form energy-rich poly3-hydroxybutyrate (PHB) at the expense of their aerobically generated polyphosphate (PP). Thus, it is expected that the PAOs containing PP sent into an anaerobic digester with volatile fatty acids (VFAs) present, would utilize their PP reserves as they would in the anaerobic zone of an AS process ending up with formation and storage of some PHB. Ultimately, all the stored products of the PAO get released, since there is no alternating aerobic environment to cater for their growth. Since it has been established that the PP release in the AD occurs much faster than the PAO biomass hydrolysis rate, it is modelled as a separate process. Steps are presented in the development of this PP release mass-balanced stoichiometries that occur with AD of PAOs. By comparing outcomes from these proposed stoichiometries against measured experimental data, it is noticed that better predictions are obtained with acetate uptake for PHB formation than when modelling the AD PP release to occur with PAO death and hydrolysis.

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Modulation of the phosphate-starvation response in Escherichia coli by genetic manipulation of the polyphosphate pathways

Sharfstein¹,

Dien²,

Keasling³

2000

Biotechnol. Bioeng.

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The effect of intracellular polyphosphate on the phosphate‐starvation response in Escherichia coli was studied by genetically manipulating the intracellular polyphosphate levels and by performing phosphate shifts on the genetically engineered strains. Strains that produced large quantities of polyphosphate and were able to degrade it induced the phosphate‐starvation response to a lesser extent than wild‐type strains, whereas strains that were unable to degrade a large intracellular polyphosphate pool induced the phosphate‐starvation response to a greater extent than wild‐type strains. These results have important implications for expression of heterologous genes under control of the phoA promoter. © 1996 John Wiley & Sons, Inc.

show abstract

Generation of a proton motive force by the excretion of metal-phosphate in the polyphosphate-accumulating Acinetobacter johnsonii strain 210A.

Cited by 66 publications

References 37 publications

Relationships between Polyphosphate Chemistry, Biochemistry and Apatite Biomineralization

Relationships between Polyphosphate Chemistry, Biochemistry and Apatite Biomineralization

Plantwide modelling – anaerobic digestion of waste sludge from parent nutrient (N and P) removal systems

Modulation of the phosphate-starvation response in Escherichia coli by genetic manipulation of the polyphosphate pathways

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