Generation of an Osteogenic Graft From Human Placenta and Placenta-Derived Mesenchymal Stem Cells

Mohr, Stefan; Portmann-Lanz, C. Bettina; Schoeberlein, Andreina; Sager, Ruth; Surbek, Daniel

doi:10.1177/1933719110377471

Cited by 22 publications

(14 citation statements)

References 47 publications

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“…Chorionic mesenchymal stem cells can undergo osteogenic [54] and neuronal differentiation [55]; yet the stemness of chorionic mesenchymal cells is uncertain at this time. Immunofluorescence studies have not demonstrated SSEA-3 and SSEA-4 in these cells [56].…”

Section: Discussionmentioning

confidence: 99%

“Trophoblast islands of the chorionic connective tissue” (TICCT): A novel placental histologic feature

Hong¹,

Romero²,

Kusanovic³

et al. 2013

Placenta

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Introduction We found isolated or clustered trophoblasts in the chorionic connective tissue of the extraplacental membranes, and defined this novel histologic feature as the “trophoblast islands of the chorionic connective tissue” (TICCT). This study was conducted to determine the clinical significance of TICCT. Methods Immunohistochemistry for cytokeratin-7 was performed on the chorioamniotic membranes (N=2155) obtained from singleton pregnancies of 1199 uncomplicated term and 956 preterm deliveries. The study groups comprised 1236 African-American and 919 Hispanic women. Gestational age ranged from 24+0 weeks to 41+6 weeks. Multiple logistic regression analysis was performed to investigate the magnitude of association between patient characteristics and the presence of TICCT. Results The likelihood of TICCT was significantly associated with advancing gestational age both in term (OR: 1.29, 95% CI: 1.16–1.45, p<0.001) and preterm deliveries (OR: 1.19, 95% CI: 1.07–1.32, p=0.001). Hispanic women were less likely than African-American women to have TICCT across gestation in term (OR: 0.23, 95% CI: 0.18–0.31, p<0.001) and preterm pregnancies (OR: 0.41, 95% CI: 0.29–0.58, p<0.001). Women with a female fetus were significantly more likely to have TICCT than women with a male fetus, in both term (OR: 1.64, 95% CI: 1.28–2.11, p<0.001) and preterm gestations (OR: 2.04, 95% CI: 1.46–2.85, p<0.001). TICCT was 40% less frequent in the presence of chronic placental inflammation [term (OR: 0.60, 95% CI: 0.45–0.81, p=0.001) and preterm gestations (OR: 0.58, 95% CI: 0.40–0.84, p=0.003)] and in parous women at term (OR: 0.60, 95% CI: 0.44–0.81, p=0.001). Conclusions Our findings suggest that the duration of pregnancy, fetal sex, and parity may influence the behavior of extravillous trophoblast and placental mesenchymal cells.

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Section: Discussionmentioning

confidence: 99%

“Trophoblast islands of the chorionic connective tissue” (TICCT): A novel placental histologic feature

Hong¹,

Romero²,

Kusanovic³

et al. 2013

Placenta

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“…There is however, limited work on placenta-derived stem cells. Surbek's group showed both first trimester and term chorionic stem cells were capable of generating osteogenic grafts [67], indicating the potential of these cells in regenerative medicine. Furthermore, another study showed engraftment of term placental adherent cells inhibited growth of multiple myeloma in bone and stimulated bone formation in an immune-deficient model of medullary myeloma bone disease [68].…”

Section: Discussionmentioning

confidence: 99%

Ontological Differences in First Compared to Third Trimester Human Fetal Placental Chorionic Stem Cells

et al. 2012

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Human mesenchymal stromal/stem cells (MSC) isolated from fetal tissues hold promise for use in tissue engineering applications and cell-based therapies, but their collection is restricted ethically and technically. In contrast, the placenta is a potential source of readily-obtainable stem cells throughout pregnancy. In fetal tissues, early gestational stem cells are known to have advantageous characteristics over neonatal and adult stem cells. Accordingly, we investigated whether early fetal placental chorionic stem cells (e-CSC) were physiologically superior to their late gestation fetal chorionic counterparts (l-CSC). We showed that e-CSC shared a common phenotype with l-CSC, differentiating down the osteogenic, adipogenic and neurogenic pathways, and containing a subset of cells endogenously expressing NANOG, SOX2, c-MYC, and KLF4, as well as an array of genes expressed in pluripotent stem cells and primordial germ cells, including CD24, NANOG, SSEA4, SSEA3, TRA-1-60, TRA-1-81, STELLA, FRAGILIS, NANOS3, DAZL and SSEA1. However, we showed that e-CSC have characteristics of an earlier state of stemness compared to l-CSC, such as smaller size, faster kinetics, uniquely expressing OCT4A variant 1 and showing higher levels of expression of NANOG, SOX2, c-MYC and KLF4 than l-CSC. Furthermore e-CSC, but not l-CSC, formed embryoid bodies containing cells from the three germ layer lineages. Finally, we showed that e-CSC demonstrate higher tissue repair in vivo; when transplanted in the osteogenesis imperfecta mice, e-CSC, but not l-CSC increased bone quality and plasticity; and when applied to a skin wound, e-CSC, but not l-CSC, accelerated healing compared to controls. Our results provide insight into the ontogeny of the stemness phenotype during fetal development and suggest that the more primitive characteristics of early compared to late gestation fetal chorionic stem cells may be translationally advantageous.

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“…In the present study, P-MSCs cultured in hCBS were differentiated into mesenchymal and ectodermal lineages, indicative of multiple/pluri-potent characteristics. A recent study showed that P-MSCS were less prone to osteogenic differentiation and expressed a lower level of CD146 when compared with MB-MSCs (34), yet another study showed successful generation of an osteogenic graft from P-MSCs (35). It appears that the expression of phenotypes and functions by P-MSCs may vary when being cultured in different conditions or being isolated from different tissue origins.…”

Section: Discussionmentioning

confidence: 99%

Characterization of placenta-derived mesenchymal stem cells cultured in autologous human cord blood serum

Wang

Yang

Zhu

et al. 2012

Molecular Medicine Reports

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Human placenta-derived mesenchymal stem cells (P-MSCs) have drawn increasing attention in the field of stem cell research due to their potential in clinical applications as well as their rich and easy to procure cell source. While studies demonstrating the potential of P-MSCs for therapeutic transplantations have been documented, a clinically compliant procedure for P-MSC expansion in vitro has yet to be established. To this end, previous studies have demonstrated that MSCs of bone marrow and cord blood origins cultured in human cord blood serum (hCBS) are comparable to those cultured in fetal bovine serum (FBS), indicating that hCBS may be an alternative to FBS for the development of in vitro cell expansion procedures free of animal components. However, stem cells from origins other than bone marrow or cord blood, particularly from human placental tissues, which have demonstrated a good potential for clinical applications, have not been characterized under similar conditions. In this study, in an attempt to define a clinically compliant protocol for P-MSC expansion in vitro, we examined the effects of human hCBS as a replacement for FBS on cell proliferation capacity, differentiation potential, MSC-specific phenotypic expression and the genetic stability of P-MSCs in cultures. P-MSCs expanded in vitro in autologous hCBS maintained the capacity of self‑renewal and expressed surface antigens characteristic of bone marrow-derived mesenchymal stem cells. Under differentiation conditions, the P-MSCs expanded in hCBS developed into adipogenic, osteogenic and neurogenic cell phenotypes. Chromosomal karyotyping and single cell gel electrophoresis analysis demonstrated that P-MSCs cultured in autologous hCBS were genetically stable. These results suggest that autologous hCBS may be used as an alternative to FBS for the in vitro expansion of P-MSCs for clinical applications.

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Generation of an Osteogenic Graft From Human Placenta and Placenta-Derived Mesenchymal Stem Cells

Cited by 22 publications

References 47 publications

“Trophoblast islands of the chorionic connective tissue” (TICCT): A novel placental histologic feature

“Trophoblast islands of the chorionic connective tissue” (TICCT): A novel placental histologic feature

Ontological Differences in First Compared to Third Trimester Human Fetal Placental Chorionic Stem Cells

Characterization of placenta-derived mesenchymal stem cells cultured in autologous human cord blood serum

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