Generation of antioxidant peptides from soy protein isolate through psychrotrophic Chryseobacterium sp. derived alkaline broad temperature active protease

Mukhia, Srijana; Kumar, Anil; Kumar, Rakshak

doi:10.1016/j.lwt.2021.111152

Cited by 30 publications

(18 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have been classifying the psychrotrophic bacteria from the East Rathong glacier of Sikkim Himalaya to maintain its repository of genomic data [7,[11][12][13][14][15][16], as well as to study its bioprospection potential [17,18]. While exploring the supraglacial site, we observed taxonomic ambiguity among a dominant group of Pseudomonas sp.…”

Section: Introductionmentioning

confidence: 99%

Multilocus sequence based identification and adaptational strategies of Pseudomonas sp. from the supraglacial site of Sikkim Himalaya

et al. 2022

Self Cite

View full text Add to dashboard Cite

Microorganisms inhabiting the supraglacial ice are biotechnologically significant as they are equipped with unique adaptive features in response to extreme environmental conditions of high ultraviolet radiations and frequent freeze-thaw. In the current study, we obtained eleven strains of Pseudomonas from the East Rathong supraglacial site in Sikkim Himalaya that showed taxonomic ambiguity in terms of species affiliation. Being one of the most complex and diverse genera, deciphering the correct taxonomy of Pseudomonas species has always been challenging. So, we conducted multilocus sequence analysis (MLSA) using five housekeeping genes, which concluded the taxonomic assignment of these strains to Pseudomonas antarctica. This was further supported by the lesser mean genetic distances with P. antarctica (0.73%) compared to P. fluorescens (3.65%), and highest ANI value of ~99 and dDDH value of 91.2 of the representative strains with P. antarctica PAMC 27494. We examined the multi-tolerance abilities of these eleven Pseudomonas strains. Indeed the studied strains displayed significant tolerance to freezing for 96 hours compared to the mesophilic control strain, while except for four strains, seven strains exhibited noteworthy tolerance to UV-C radiations. The genome-based findings revealed many cold and radiation resistance-associated genes that supported the physiological findings. Further, the bacterial strains produced two or more cold-active enzymes in plate-based assays. Owing to the polyadaptational attributes, the strains ERGC3:01 and ERGC3:05 could be most promising for bioprospection.

show abstract

Section: Introductionmentioning

confidence: 99%

Multilocus sequence based identification and adaptational strategies of Pseudomonas sp. from the supraglacial site of Sikkim Himalaya

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, Zn 2+ exhibited significant inhibition, retaining only 5.94% of enzyme activity. Ca 2+ and Mn 2+ maintained the native conformation of the protease by limiting its folding, thereby enhancing its stability and proteolytic activity [ 32 ], whereas other metal ions altered the initial structure of the enzyme, resulting in a decrease in enzyme activity [ 33 ]. The concentration of metal ions had a greater effect on protease activity.…”

Section: Resultsmentioning

confidence: 99%

“…Several denaturants and protease inhibitors can inhibit enzymes. The enzyme activity was generally reduced in the presence of the surfactant [ 32 ], retaining 77.83% of the enzyme activity in the presence of TritonX-100 and an increase of 19.9% in Tween 80. In organic solvents, ethyl acetate strongly inhibited enzyme activity while 10% final concentrations of methanol, dimethyl sulfoxide, and ethanol promoted enzyme activity.…”

Section: Resultsmentioning

confidence: 99%

Screening and Characteristics of Marine Bacillus velezensis Z-1 Protease and Its Application of Enzymatic Hydrolysis of Mussels to Prepare Antioxidant Active Substances

et al. 2022

Molecules

View full text Add to dashboard Cite

Bacillus velezensis is a type of microorganism that is beneficial to humans and animals. In this work, a protease-producing B. velezensis strain Z-1 was screened from sludge in the sea area near Qingdao (deposit number CGMCC No. 25059). The response surface methodology was used to analyze protease production, and the optimal temperature was 37.09 °C and pH 7.73 with the addition of 0.42% NaCl, resulting in maximum protease production of 17.64 U/mL. The optimum reaction temperature and pH of the protease of strain Z-1 were 60 °C and 9.0, respectively. The protease had good temperature and pH stability, and good stability in solvents such as methanol, ethanol and Tween 80. Ammonium, NH4+,and Mn2+ significantly promoted enzyme activity, while Zn2+ significantly inhibited the enzyme activity. The protease produced by strain Z-1 was used for the enzymolysis of mussel meat. The mussel hydrolysate exhibited good antioxidant function, with a DPPH free radical removal rate of 75.3%, a hydroxyl free radical removal rate of 75.9%, and a superoxide anion removal rate of 84.4%. This study provides a reference for the application of B. velez protease and the diverse processing applications of mussel meat.

show abstract

“…Several factors influence the antioxidant activities of food-derived peptides, including the parent protein source, amino acid composition, sequence, chain length, hydrophobicity, and preparation method such enzymatic and microbial hydrolysis [ 18 , 21 ]. Different peptides with antioxidant properties have been produced from enzymatic hydrolysis of milk and plant proteins using a variety of proteases from plant, animal and microbial sources [ 22 , 23 , 24 , 25 , 26 ]. Notably, low molecular weight peptides (containing 2–15 amino acid residues) appear to be more bioaccessible and, hence, may potentiate better practical health-promoting effects in vivo [ 27 , 28 , 29 , 30 ].…”

Section: Overview Of Food-derived Antioxidant Peptides (Fdaps)mentioning

confidence: 99%

Transepithelial transport and cellular mechanisms of food-derived antioxidant peptides

Okagu

Udenigwe²

2022

Heliyon

View full text Add to dashboard Cite

Generation of antioxidant peptides from soy protein isolate through psychrotrophic Chryseobacterium sp. derived alkaline broad temperature active protease

Cited by 30 publications

References 43 publications

Multilocus sequence based identification and adaptational strategies of Pseudomonas sp. from the supraglacial site of Sikkim Himalaya

Multilocus sequence based identification and adaptational strategies of Pseudomonas sp. from the supraglacial site of Sikkim Himalaya

Screening and Characteristics of Marine Bacillus velezensis Z-1 Protease and Its Application of Enzymatic Hydrolysis of Mussels to Prepare Antioxidant Active Substances

Transepithelial transport and cellular mechanisms of food-derived antioxidant peptides

Contact Info

Product

Resources

About