Generation of Artificial Gamete and Embryo From Stem Cells in Reproductive Medicine

Zhang, Pu-Yao; Fan, Yong; Tan, Tao; Yu, Yang

doi:10.3389/fbioe.2020.00781

Cited by 23 publications

(10 citation statements)

References 141 publications

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“…In the last decade, the prevalence of infertility has significantly increased in the western world. Today, about 8–15% of individuals of reproductive age willing to conceive are supposed to be infertile [ 110 , 112 ]. Infertility is defined upon verification of a specific impairment of a person´s capacity to reproduce or of the failure to achieve a pregnancy after a period of 12 months of unprotected sexual intercourse [ 137 ].…”

Section: Multipotent Fetal Stem Cells As New Therapeutic Tools For Re...mentioning

confidence: 99%

“…At present, two different strategies using stem cells for infertility treatment are pursued: the transplantation of stem cells or stem cell-derived paracrine factors to restore reproductive organ functions, which is discussed in the next chapter, and the in vitro differentiation of stem cells into germ cells or gametes [ 109 , 110 , 112 ].…”

Section: Multipotent Fetal Stem Cells As New Therapeutic Tools For Re...mentioning

confidence: 99%

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Multipotent fetal stem cells in reproductive biology research

et al. 2023

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Due to the limited accessibility of the in vivo situation, the scarcity of the human tissue, legal constraints, and ethical considerations, the underlying molecular mechanisms of disorders, such as preeclampsia, the pathological consequences of fetomaternal microchimerism, or infertility, are still not fully understood. And although substantial progress has already been made, the therapeutic strategies for reproductive system diseases are still facing limitations. In the recent years, it became more and more evident that stem cells are powerful tools for basic research in human reproduction and stem cell-based approaches moved into the center of endeavors to establish new clinical concepts. Multipotent fetal stem cells derived from the amniotic fluid, amniotic membrane, chorion leave, Wharton´s jelly, or placenta came to the fore because they are easy to acquire, are not associated with ethical concerns or covered by strict legal restrictions, and can be banked for autologous utilization later in life. Compared to adult stem cells, they exhibit a significantly higher differentiation potential and are much easier to propagate in vitro. Compared to pluripotent stem cells, they harbor less mutations, are not tumorigenic, and exhibit low immunogenicity. Studies on multipotent fetal stem cells can be invaluable to gain knowledge on the development of dysfunctional fetal cell types, to characterize the fetal stem cells migrating into the body of a pregnant woman in the context of fetomaternal microchimerism, and to obtain a more comprehensive picture of germ cell development in the course of in vitro differentiation experiments. The in vivo transplantation of fetal stem cells or their paracrine factors can mediate therapeutic effects in preeclampsia and can restore reproductive organ functions. Together with the use of fetal stem cell-derived gametes, such strategies could once help individuals, who do not develop functional gametes, to conceive genetically related children. Although there is still a long way to go, these developments regarding the usage of multipotent fetal stem cells in the clinic should continuously be accompanied by a wide and detailed ethical discussion.

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Section: Multipotent Fetal Stem Cells As New Therapeutic Tools For Re...mentioning

confidence: 99%

Section: Multipotent Fetal Stem Cells As New Therapeutic Tools For Re...mentioning

confidence: 99%

Multipotent fetal stem cells in reproductive biology research

et al. 2023

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“…Further investigation to the potential growth, culture and developmental competence of these cells would be required to determine the nature of their pluripotency. Recent work has also established the potential of complete male and female gamete generation in vitro in multiple species, including from stem cells [52][53][54][55]. Furthermore, mice have been produced with the genetic material from cells originating from two male mice [56].…”

Section: Plos Onementioning

confidence: 99%

Live births from urine derived cells

Olsson¹,

Yeonwoo²,

Park

et al. 2023

PLoS ONE

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Here we report urine-derived cell (UDC) culture and subsequent use for cloning which resulted in the successful development of cloned canine pups, which have remained healthy into adulthood. Bovine UDCs were used in vitro to establish comparative differences between cell sources. UDCs were chosen as a readily available and noninvasive source for obtaining cells. We analyzed the viability of cells stored in urine over time and could consistently culture cells which had remained in urine for 48hrs. Cells were shown to be viable and capable of being transfected with plasmids. Although primarily of epithelial origin, cells were found from multiple lineages, indicating that they enter the urine from more than one source. Held in urine, at 4°C, the majority of cells maintained their membrane integrity for several days. When compared to in vitro fertilization (IVF) derived embryos or those from traditional SCNT, UDC derived embryos did not differ in total cell number or in the number of DNA breaks, measured by TUNEL stain. These results indicate that viable cells can be obtained from multiple species’ urine, capable of being used to produce live offspring at a comparable rate to other cell sources, evidenced by a 25% pregnancy rate and 2 live births with no losses in the canine UDC cloning trial. This represents a noninvasive means to recover the breeding capacity of genetically important or infertile animals. Obtaining cells in this way may provide source material for human and animal studies where cells are utilized.

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“…However there is no report of human somatic cells ability to differentiate into haploid spermatid. Human granulosa cells are somatic cells that have stem cells characteristics (27). Oct-4 which its role in remaining self-renewing ability of stem cells and their pluripotency is known has expression in unfertilized oocytes (28).…”

Section: Declarations Declaration Of Interestmentioning

confidence: 99%

The differentiation potential of human granulosa cells into sperm-like cells

Ghadirkhomi

Maleki

ghadirkhomi³

2022

Preprint

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Context: According to previous studies human-derived luteal phase granulosa cells are able to differentiate into other cell lineages, such as osteoblasts, chondrocytes and neurons. Aims: In this study we investigated the differentiation potential of these cells into sperm-like cells. Methods: First, we demonstrated the stemness of isolated GCs by flow cytometry and then we showed that granulosa cells can differentiate into advanced male germ cell lineages including spermatid-like cells in vitro. Follicular-phase GCs were cultured then induced by sheep testis tissue extract to differentiate into sperm-like cells, as evidenced by their expression of Gpr125, as well as VASA and DAZL, which are markers of different steps of Spermatogenesis.Key results: Mesenchymal stem cell markers were expressed in these cells. Spermatogenesis markers including DAZL,VASA and GPR125, were also expressed from the second week after induction. Morphological analysis of induced cells showed that Producing sperm- like cellsweresimilar to the normal sperm. Conclusions: Obtained results prove that isolated GCs are a kind of mesenchymal stem cells and have the capability to differentiate into sperm–like cells. Implications: This finding may represent a novel strategy for studying spermatogenesis in vitro or may help to improve assisted reproductive technology methods in the future.

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Generation of Artificial Gamete and Embryo From Stem Cells in Reproductive Medicine

Cited by 23 publications

References 141 publications

Multipotent fetal stem cells in reproductive biology research

Multipotent fetal stem cells in reproductive biology research

Live births from urine derived cells

The differentiation potential of human granulosa cells into sperm-like cells

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