Generation of bovine immune colostrum against Streptococcus mutans and Streptococcus sobrinus and its effect on glucose uptake and extracellular polysaccharide formation by mutans streptococci

Loimaranta, Vuokko; Tenovuo, Jorma; Virtanen, Susanna; Marnila, Pertti; Syväoja, Eeva-Liisa; Tupasela, Tuomo; Korhonen, Hannu J. T.

doi:10.1016/s0264-410x(97)00027-3

Cited by 32 publications

(22 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…IgG is the major class of immunoglobulin present in ruminant milk, whereas IgA is the major immunoglobulin class present in human milk [18,19]. The immunological activity of IgGs in milk from cows immunized against human pathogens is similar to that of IgGs in human milk, which indicates the potential benefits of hyperimmune bovine milk in the human diet [20,21]. Recent studies have shown that daily supplementation of skimmed milk from cows immunized with human enteropathogenic microorganisms can reduce elevated blood cholesterol concentrations in patients with primary hypercholesterolaemia [22].…”

Section: Introductionmentioning

confidence: 97%

Oral administration of immunoglobulin G-enhanced colostrum alleviates insulin resistance and liver injury and is associated with alterations in natural killer T cells

Adar

Ya’acov

Lalazar

et al. 2012

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SummaryInsulin resistance and metabolic syndrome are chronic inflammatory conditions that lead to hepatic injury and non-alcoholic steatohepatitis (NASH). Bovine colostrum has therapeutic effects in a variety of chronic infections. However its effectiveness in NASH was never studied. Natural killer T (NKT) cells have been shown to be associated with some of the pathological and metabolic abnormalities accompanying NASH in leptin-deficient (ob/ob) mice. In the present study, we used hyperimmune bovine colostrum to treat hepatic injury and insulin resistance and we also assessed the effects on NKT cells. We used ob/ob mice that were fed for 6 weeks with either 0·1 mg bovine colostrum prepared from non-immunized cows, 0·1 mg hyperimmune colostrum raised against a bacterial lipopolysaccharide (LPS) extract or 0·001, 0·1 or 1 mg of immunoglobulin (Ig)G purified from hyperimmune colostrum (IgG-LPS). NKT cells were phenotyped by flow cytometry, and hepatic injury and insulin resistance were assessed by measuring fasting glucose levels, glucose tolerance tests and liver enzymes. Fat accumulation was measured in the liver and plasma. Oral administration of hyperimmune colostrums decreased alanine aminotransferase (ALT) serum levels and serum triglycerides compared to controls. Glucose intolerance was also improved by the hyperimmune colostrum preparations. These results were accompanied by a decrease in serum tumour necrosis factor (TNF)-a levels following oral treatment with 0·1 or 1 mg of IgG-LPS. The beneficial effects of hyperimmune colostrums were associated with an increase in the number of splenic NKT cells. These data suggest that oral administration of hyperimmune colostrum preparations can alleviate chronic inflammation, liver injury and insulin resistance associated with NASH.

show abstract

Section: Introductionmentioning

confidence: 97%

Oral administration of immunoglobulin G-enhanced colostrum alleviates insulin resistance and liver injury and is associated with alterations in natural killer T cells

Adar

Ya’acov

Lalazar

et al. 2012

Clinical and Experimental Immunology

View full text Add to dashboard Cite

show abstract

“…However, it may be impractical to introduce active immunization in humans to prevent dental caries because a vaccine targeting a nonlethal disease, such as dental caries, must be safer than vaccines developed for life-threatening infections (3). Therefore, passive immunization for the prevention of dental caries has received much attention (3,5,25), and monoclonal antibodies (17), chicken egg yolk antibodies (6,24), bovine milk antibodies (2,16), and synthetic peptide (7) have been used. In those experiments, IgG, chicken egg yolk IgY, and synthetic peptides were purified and applied directly to the oral cavity or applied as a mixture with diet and drinking water.…”

mentioning

confidence: 99%

Passive Immunization with Bovine Milk Containing Antibodies to a Cell Surface Protein Antigen-Glucosyltransferase Fusion Protein Protects Rats against Dental Caries

et al. 2002

View full text Add to dashboard Cite

Cell surface protein antigen (PAc) and glucosyltransferases (GTF) of Streptococcus mutans are major colonization factors of the organism. We prepared bovine milk containing antibodies against a fusion of the saliva-binding alanine-rich region of PAc with the glucan-binding domain of GTF-I. This study examined the effect of the immune milk on the cariogenicity of S. mutans in a rat model. Concentrated immune milk was fed to rats once a day for 55 days. The group that received immune milk had significantly less caries development than controls.

show abstract

“…Passive immunization for prevention of dental caries has recently received much attention (3,4,23), and monoclonal antibodies, chicken egg yolk antibodies, and bovine milk antibodies have been used (1,6,13,14). Of these, bovine milk antibodies are, in theory, most easily obtained on a large scale.…”

Section: Discussionmentioning

confidence: 99%

“…Of these, bovine milk antibodies are, in theory, most easily obtained on a large scale. In practice, colostrum or concentrated milk has been used in previous studies (1,13,17) because of the difficulty of obtaining normal milk containing high titers of antibodies. However, the use of colostrum as a daily food for humans is prohibited by the Ministry of Health, Labour and Welfare in Japan, because colostrum contains a large amount of proteins, including blood cells, readily forms a coagulum on warming, and is colored.…”

Section: Discussionmentioning

confidence: 99%

Passive Immunization with Milk Produced from an Immunized Cow Prevents Oral Recolonization by Streptococcus mutans

Shimazaki

Mitoma

Oho

et al. 2001

Clin Diagn Lab Immunol

View full text Add to dashboard Cite

Cell surface protein antigen (PAc) and water-insoluble glucan-synthesizing enzyme (GTF-I) produced by cariogenic Streptococcus mutans are two major factors implicated in the colonization of the human oral cavity by this bacterium. We examined the effect of bovine milk, produced after immunization with a fusion protein of functional domains of these proteins, on the recolonization of S. mutans. To prepare immune milk, a pregnant Holstein cow was immunized with the fusion protein PAcA-GB, a fusion of the saliva-binding alanine-rich region (PAcA) of PAc and the glucan-binding (GB) domain of GTF-I. After eight adult subjects received cetylpyridinium chloride (CPC) treatment, one subgroup (n ‫؍‬ 4) rinsed their mouths with immune milk and a control group (n ‫؍‬ 4) rinsed with nonimmune milk. S. mutans levels in saliva and dental plaque decreased after CPC treatment in both groups. Mouth rinsing with immune milk significantly inhibited recolonization of S. mutans in saliva and plaque. On the other hand, the numbers of S. mutans cells in saliva and plaque in the control group increased immediately after the CPC treatment and surpassed the baseline level 42 and 28 days, respectively, after the CPC treatment. The ratios of S. mutans to total streptococci in saliva and plaque in the group that received immune milk were lower than those in the control group. These results suggest that milk produced from immunized cow may be useful for controlling S. mutans in the human oral cavity.

show abstract

Generation of bovine immune colostrum against Streptococcus mutans and Streptococcus sobrinus and its effect on glucose uptake and extracellular polysaccharide formation by mutans streptococci

Cited by 32 publications

References 48 publications

Oral administration of immunoglobulin G-enhanced colostrum alleviates insulin resistance and liver injury and is associated with alterations in natural killer T cells

Oral administration of immunoglobulin G-enhanced colostrum alleviates insulin resistance and liver injury and is associated with alterations in natural killer T cells

Passive Immunization with Bovine Milk Containing Antibodies to a Cell Surface Protein Antigen-Glucosyltransferase Fusion Protein Protects Rats against Dental Caries

Passive Immunization with Milk Produced from an Immunized Cow Prevents Oral Recolonization by Streptococcus mutans

Contact Info

Product

Resources

About