Generation of cell adhesive substrates using peptide fluoralkyl surface modifiers

Ernsting, Mark J.; Bonin, Geneviève C.; Yang, Meilin; Labow, Rosalind S.; Santerre, J. Paul

doi:10.1016/j.biomaterials.2005.04.047

Cited by 33 publications

(59 citation statements)

References 46 publications

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“…The amount of peptides on the modified PU film was quantitatively analyzed by amino acid analysis (AAA). 29,30 The film was placed into a glass tube, added 1 mL of 6 N HCl, and vacuum sealed. The tube was hydrolyzed at 110 o C for 24 h. Free amino acids were analyzed by a high-performance liquid chromatograph (HPLC, Hewlett Packard 1100 Series, Waldbom, Germany), equipped with a reverse-phase column (Waters symmetry C18).…”

Section: Methodsmentioning

confidence: 99%

Fabrication of endothelial cell-specific polyurethane surfaces co-immobilized with GRGDS and YIGSR peptides

et al. 2009

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Polyurethane (PU) is widely used as a cardiovascular biomaterial due to its good mechanical properties and hemocompatibility, but it is not adhesive to endothelial cells (ECs). Cell adhesive peptides, GRGDS and YIGSR, were found to promote adhesion and spreading of ECs and showed a synergistic effect when both of them were used. In this study, a surface modification was designed to fabricate an EC-active PU surface capable of promoting endothelialization using the peptides and poly(ethylene glycol) (PEG) spacer, The modified PU surfaces were characterized in vitro. The density of the grafted PEG on the PU surface was measured by acid-base back titration to the terminal-free isocyanate groups. The successful immobilization of peptides was confirmed by amino acid analysis, following hydrolysis, and contact angle measurement. The uniform distribution of peptides on the surface was observed by scanning electron microscopy (SEM) and atomic force microscopy (AFM). To evaluate the EC adhesive property, cell viability test using human umbilical vein EC (HUVEC) was investigated in vitro and enhanced endothelialization was characterized by the introduction of cell adhesive peptides, GRGDS and YIGSR, and PEG spacer. Therefore, GRGDS and YIGSR co-immobilized PU surfaces can be applied to an EC-specific vascular graft with long-term patency by endothelialization.

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Section: Methodsmentioning

confidence: 99%

Fabrication of endothelial cell-specific polyurethane surfaces co-immobilized with GRGDS and YIGSR peptides

et al. 2009

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“…F-alkyl moieties can modify the surface of polymer, including ePTFE, reduce thrombonicity and friction, protect from enzymatic degradation, and promote attachment and growth of specific cells (Ernsting et al, 2005;Larsen et al, 2006).…”

Section: Uses and Potential In Theranosticsmentioning

confidence: 99%

Selected physicochemical aspects of poly- and perfluoroalkylated substances relevant to performance, environment and sustainability—Part one

Krafft

Riess²

2015

Chemosphere

224

119

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“…Therefore, the performance of a hydrogel material relies highly upon the properties of the boundaries in tissue engineering applications because the interactions between the hydrogel materials and environments occur mainly on its surfaces [1][2][3][4]. The hydroxyethyl methacrylate (pHEMA) hydrogel possesses good biocompatibility as evidenced by its large scale use in biomedical and biotechnological applications such as contact lenses, drug delivery systems, and soft tissue prosthesis [5][6][7][8][9]. On the other hand, it lacks functional groups to which biological molecules can easily be attached.…”

Section: Introductionmentioning

confidence: 99%

Poly (hydroxyethyl methacrylate-glycidyl methacrylate) films modified with different functional groups: In vitro interactions with platelets and rat stem cells

Bayramoğlu

Bitirim

Tunalı

et al. 2013

Materials Science and Engineering: C

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Keywords: Hydrogels biocompatible polymers contact angle blood cell adhesion hemolysis rat mesenchymal stem cells Copolymerization of 2-hydroxyethylmethacrylate (HEMA) with glycidylmethacrylate (GMA) in the presence of α-α′-azoisobisbutyronitrile (AIBN) resulted in the formation of hydrogel films carrying reactive epoxy groups. Thirteen kinds of different molecules with pendant \NH 2 group were used for modifications of the p(HEMA-GMA) films. The \NH 2 group served as anchor binding site for immobilization of functional groups on the hydrogel film via direct epoxy ring opening reaction. The modified hydrogel films were characterized by FTIR, and contact angle studies. In addition, mechanical properties of the hydrogel films were studied, and modified hydrogel films showed improved mechanical properties compared with the non-modified film, but they are less elastic than the non-modified film. The biological activities of these films such as platelet adhesion, red blood cells hemolysis, and swelling behavior were studied. The effect of modified hydrogel films, including \NH 2 , (using different aliphatic \CH 2 chain lengths) \CH 3 , \SO 3 H, aromatic groups with substituted \OH and \COOH groups, and amino acids were also investigated on the adhesion, morphology and survival of rat mesenchymal stem cells (MSCs). The MTT colorimetric assay reveals that the p(HEMA-GMA)-GA-AB, p(HEMA-GMA)-GA-Phe, p(HEMA-GMA)-GA-Trp, p(HEMA-GMA)-GA-Glu formulations have an excellent biocompatibility to promote the cell adhesion and growth. We anticipate that the fabricated p(HEMA-GMA) based hydrogel films with controllable surface chemistry and good stable swelling ratio may find extensive applications in future development of tissue engineering scaffold materials, and in various biotechnological areas.

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Generation of cell adhesive substrates using peptide fluoralkyl surface modifiers

Cited by 33 publications

References 46 publications

Fabrication of endothelial cell-specific polyurethane surfaces co-immobilized with GRGDS and YIGSR peptides

Fabrication of endothelial cell-specific polyurethane surfaces co-immobilized with GRGDS and YIGSR peptides

Selected physicochemical aspects of poly- and perfluoroalkylated substances relevant to performance, environment and sustainability—Part one

Poly (hydroxyethyl methacrylate-glycidyl methacrylate) films modified with different functional groups: In vitro interactions with platelets and rat stem cells

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