Generation of gamma irradiation-induced mutant lines of the miniature tomato (Solanum lycopersicum L.) cultivar 'Micro-Tom'

Matsukura, Chiaki; Yamaguchi, Isao; Inamura, Mami; Ban, Yusuke; Kobayashi, Yoshie; Yin, Yong; Saito, Takeshi; Kuwata, Chikara; Imanishi, Shunsuke; Nishimura, Shigeo

doi:10.5511/plantbiotechnology.24.39

Cited by 56 publications

(41 citation statements)

References 19 publications

(18 reference statements)

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“…Outra forma de expressar mutantes em plantas anãs é através da produção de paredes celulares anormais (REITER; CHAPPLE, SOMERVILLE, 1993) ou defeitos na célula de expansão/alongamento (TAKAHASHI et al, 1995). Ressalta-se no presente estudo que o genótipo anão utilizado não foi obtido via indução por raios gama (MATSUKURA et al, 2007). O genótipo em estudo se trata de uma planta atípica identificada ao acaso em uma população de ocorrência espontânea.…”

Section: Resultsunclassified

Ocorrência De Nanismo Em Planta De Tomateiro Do Tipo Grape

Maciel

Fernandes

2015

Rev. Caatinga

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RESUMO -O conhecimento genético de manifestações fenotípicas de características de importância agronô-mica em vegetais é uma atividade de grande importância, cujos resultados são amplamente utilizados por fitomelhoristas. O objetivo do presente trabalho foi estudar o tipo de segregação genética que ocorre ao se cruzar um genótipo anão versus genótipo de hábito de crescimento indeterminado. A referida planta foi encontrada vegetando espontaneamente no município de Piracicaba (SP) em local de descarte de frutos por tomaticultores juntamente com as plantas normais. As sementes de ambas as plantas foram colhidas separadamente para início do estudo e codificadas da seguinte forma: planta com fenótipo normal (FN) e planta com fenótipo atípico com características de porte anão (FA). A metodologia constou de cruzamentos biparentais recíprocos e retrocruzamentos da geração F 1 com o genitor FA e obtenção de geração F 2 . Os dados obtidos oriundos da contagem de plantas das populações obtidas (F 1 , F 2 e F 1 RC 1 ) foram submetidos ao teste de χ 2 de acordo com a frequência esperada e observada do fenótipo normal (FN) e planta com fenótipo atípico com características de porte anão (FA), tendo como hipótese uma segregação mendeliana 3:1, obtida para herança monogênica. Pelos resultados obtidos foi possível concluir que o fenótipo observado na planta no presente estudo é de origem genética recessiva, podendo ser transmito para outras plantas via cruzamento. Palavras-chave:Solanum lycopersicum L. Gene d (Dwarf). Herança. Alelo. Mutação. DWARFISM OCCURRENCE IN TOMATO PLANT TYPE GRAPEABSTRACT -The phenotypic manifestations of genetic knowledge of important agronomic traits in plants is an activity of great importance, whose results are widely used in plant breeding. The objective was to study the type of genetic segregation that occurs when crossing a dwarf genotype versus indeterminate growth habit genotype. Their plant was found growing spontaneously in Piracicaba (SP), on-site disposal of fruits per tomatoes producers along with normal plants. Seeds of both plants were harvested separately for baseline and coded as follows: a plant with a normal phenotype (FN) and plant with atypical phenotype with characteristics of dwarfism (FA). The methodology consisted of biparental and reciprocal backcrosses of the F 1 to the parent FA and obtained the F 2 generation. The data derived from the plant count populations obtained (F 1 , F 2 and backcrosses) were tested using the χ 2 according to the expected frequency and observed the normal phenotype (FN) and plant with atypical phenotype with characteristics of dwarfism (FA), under the hypothesis Mendelian segregation 3:1, obtained by monogenic inheritance. From the results, it can be concluded that the phenotype observed in the plant in this study is recessive genetic origin can be inserted into other plants by crossing.

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Section: Resultsunclassified

Ocorrência De Nanismo Em Planta De Tomateiro Do Tipo Grape

Maciel

Fernandes

2015

Rev. Caatinga

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“…Our group (University of Tsukuba) developed 3845 EMS mutagenized M 2 families (Watanabe et al, 2007) and 6422 gamma-ray-irradiated M 2 families (Matsukura et al, 2007) (Figs. 2A, 3A, and 3G).…”

Section: Development Of Mutant Resources Based On 'Micro-tom'mentioning

confidence: 99%

Mutant Resources for the Miniature Tomato (<i>Solanum lycopersicum</i> L.) ‘Micro-Tom’

Saito

Asamizu

Mizoguchi

et al. 2009

J. Japan. Soc. Hort. Sci.

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Mutant-based studies have contributed to the elucidation of gene functions in plants including tomato. In this review, we introduce some outstanding research performed using spontaneous and artificially induced mutants. Tomato (Solanum lycopersicum L.) is a model species in the solanaceae family and research tools and information necessary to perform functional genomics study are being developed worldwide. Saturation mutagenesis is a powerful strategy that enables exploration of gene function on a genome-wide scale. We reviewed conventional mutant resources of tomato, including the ethyl methanesulfonate (EMS)-mutagenized population of the 'M82', which was developed in Israel (Menda et al., 2004). Recently, a miniature cultivar, 'Micro-Tom', has attracted the attention of tomato researchers due to its small size and rapid life cycle, which make it an ideal experimental organism for handling in the laboratory. We are developing a unique mutant resource, 10,000 EMS-mutagenized and gamma-ray-irradiated lines derived from 'Micro-Tom'. Distinct mutant phenotypes, including plant size, leaf shape, and fruit morphology, are described. The status of newly developed tools for using this mutant resource, such as an efficient transformation method and the Targeting Induced Local Lesions In Genomes (TILLING) system, are also summarized.

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“…For this purpose, we present a case study of selection for tolerance to the heavy metal cadmium, in plants of the tomato cultivar Micro-Tom (Solanum lycopersicum L.), widely used as model plant (Meissner et al 1997) in numerous studies on genetics (Lima et al 2004, Butelli et al 2008, physiology , Monteiro et al 2011, Carvalho et al 2013, biochemistry of plants (Gratão et al 2008, and on ultrastructural changes (Vitória et al 2003, Gratão et al 2009). This tomato cultivar has several advantages, such as a low plant height (about 15 cm) and a short life cycle (80-90 days from sowing to harvest), making it a very suitable model plant for mutagenesis studies aimed at selecting new mutants (Matsukura et al 2007, Watanabe et al 2007, Pino-Nunes et al 2009). We believe that the methodology proposed here is fast and effective due to the fact that selection occurs in the seedling stage, few days after germination, allowing the evaluation of a large number of plants per area unit.…”

Section: Introductionmentioning

confidence: 99%