Generation of genetic recombinants in Trichosporon cutaneum by spontaneous segregation of protoplast fusants

Sieńko, Marzena; Pp, Stepień; Paszewski, Andrzej

doi:10.1099/00221287-138-7-1409

Cited by 7 publications

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The yeast, Trichosporon cutaneum, is an anamorphic fungi in the genus Trichosporon family Trichosporonaceae with versatile biotechnological potential, which can utilize a wide variety of carbon sources and also has an outstanding capacity to metabolize phenol and other various aromatic compounds [31][32][33][34][35]. Due to these properties, the recent studies on T. cutaneum were focused more on bioconversion of various carbon source feedstocks such as cassava starch, pectin-derived carbohydrates, corn stover and corncob residues hydrolysate into microbial lipid [35][36][37][38][39].…”

Section: Introductionmentioning

confidence: 99%

“…Due to these properties, the recent studies on T. cutaneum were focused more on bioconversion of various carbon source feedstocks such as cassava starch, pectin-derived carbohydrates, corn stover and corncob residues hydrolysate into microbial lipid [35][36][37][38][39]. Meanwhile, studies on the fundamental physiology and morphology characters of T. cutaneum are still very limited [33,40]. To the best of our knowledge, although Depree et al [40] mentioned that T. cutaneum is able to form either yeast cells or filamentous form, inducing environmental and nutritional conditions are still unclear.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Influence of environmental and nutritional conditions on yeast–mycelial dimorphic transition in Trichosporon cutaneum

Zhu

Zhang

et al. 2017

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

Trichosporon cutaneum is able to metabolize high hydrophobic natural compounds such as fatty acids and also can be used as an effective biodegrader to remove a number of toxic aromatic compounds from the environment. However, its growth characteristics were poorly investigated and no yeast-mycelium dimorphism process has been established yet. In the present study, we provided first insights into the effect of nitrogen sources, carbon source, and amino acids together with pH and temperature on morphological switch of T. cutaneum B3. The results showed under close to neutral or weakly alkaline pH conditions, T. cutaneum B3 produced mostly yeast-like cells; while under acidic pH conditions, it produced mostly hyphal-like cells. Under buffered conditions, low nitrogen concentration (<0.2%) would facilitate T. cutaneum B3 to produce yeast-like cells, while the presence of relative high nitrogen concentration (>1%) would induce hyphal-like cells. Under non-buffered conditions, ammonium sulphate, diammonium phosphate, urea and Nacetylglucosamine may via alteration of environmental pH affect yeast-mycelium dimorphism transitions. Methionine, tryptophan and histidine invariably induce pseudohyphal or hyphal morphology. 25 and 28 C can promote yeast-like cells growth, while cultivated at 37 C can induce hyphal-like cells growth. Thus, the nitrogen source, alteration of environmental pH and temperature of cultivation played an important role in inducing yeast-mycelium dimorphism transition. Our study confirms the yeast-mycelium dimorphism process of T. cutaneum B3 and highlights that it seems to be a suitable yeast model for further molecular genetics investigation of dimorphism and applications in fermentation morphology engineering.

show abstract