Generation of inositol phosphates in bitter taste transduction

Spielman, Andrew I.; Huque, Taufiqul; Nagai, Hirokazu; Whitney, Glayde; Brand, Joseph G.

doi:10.1016/0031-9384(94)90359-x

Cited by 75 publications

(55 citation statements)

References 22 publications

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“…The results support the hypothesis that denatonium increases [Ca 2ϩ ] i via a G-protein cascade involving PLC and IP 3 (Hwang et al, 1990;Spielman et al, 1994b). Our evidence does not support the hypothesis that denatonium is transduced via a pathway involving phosphodiesterase and membrane depolarization via a cyclic nucleotide-suppressible cation channel (Kolesnikov and Margolskee, 1995;Wong et al, 1996).…”

Section: Discussioncontrasting

confidence: 64%

“…Denatonium increases [Ca 2ϩ ] i in some rat taste cells (Akabas et al, 1988;Bernhardt et al, 1996). In mouse circumvallate and foliate papillae, denatonium activates PLC (Spielman et al, 1994b).…”

Section: Discussionmentioning

confidence: 99%

“…Unexpected perhaps is evidence that the bittertasting denatonium ion may operate via two separate transduction pathways. One hypothesis proposes that denatonium activates phospholipase C (PLC), thus increasing intracellular levels of inositol 1,4,5-trisphosphate (IP 3 ) to release calcium ions from intracellular stores (Akabas et al, 1988;Hwang et al, 1990;Spielman et al, 1994b). The increase in intracellular calcium is proposed to lead to neurotransmitter release, thereby completing the transduction step (Akabas et al, 1988;Spielman et al, 1994b).…”

Section: Abstract: Bitter Taste Transduction; Mudpuppy; Taste Receptmentioning

confidence: 99%

“…One hypothesis proposes that denatonium activates phospholipase C (PLC), thus increasing intracellular levels of inositol 1,4,5-trisphosphate (IP 3 ) to release calcium ions from intracellular stores (Akabas et al, 1988;Hwang et al, 1990;Spielman et al, 1994b). The increase in intracellular calcium is proposed to lead to neurotransmitter release, thereby completing the transduction step (Akabas et al, 1988;Spielman et al, 1994b). Another hypothesis is that denatonium activates phosphodiesterase to decrease intracellular levels of cyclic nucleotides (RuizAvila et al, 1995;Wong et al, 1996), which would depolarize the cell by relieving the cyclic nucleotide block of a membrane cation channel (Kolesnikov and Margolskee, 1995).…”

Section: Abstract: Bitter Taste Transduction; Mudpuppy; Taste Receptmentioning

confidence: 99%

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Bitter Taste Transduction of Denatonium in the MudpuppyNecturus maculosus

1997

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Bitter substances are a structurally diverse group of compounds that appear to act via several transduction mechanisms. The bitter-tasting denatonium ion has been proposed to act via two different G-protein-regulated pathways, one involving inositol 1,4,5-trisphosphate and raised intracellular calcium levels, the other involving phosphodiesterase and membrane depolarization via a cyclic nucleotide-suppressible cation channel. The aim of the present study was to examine these transduction mechanisms in taste cells of the mudpuppy Necturus maculosus by calcium-imaging and whole-cell recording. Denatonium benzoate increased intracellular calcium levels and induced an outward current independently of extracellular calcium. The denatonium-induced increase in intracellular calcium was inhibited by U73122, an inhibitor of phospholipase C, and by thapsigargin, an inhibitor of calcium transport into intracellular stores. The denatonium-induced outward current was blocked by GDP-␤-S, a blocker of G-protein activation. Neither resting nor denatonium-induced intracellular calcium levels were affected by inhibition of phosphodiesterase (with IBMX ) or adenylate cyclase (with SQ22536) or by raising intracellular cyclic nucleotides directly (with cell permeant analogs). Our results support the hypothesis that denatonium is transduced via a G-protein cascade involving phospholipase C, inositol 1,4,5-trisphosphate, and raised intracellular calcium levels. Our results do not support the hypothesis that denatonium is transduced via phosphodiesterase and cAMP.

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Section: Discussioncontrasting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Section: Abstract: Bitter Taste Transduction; Mudpuppy; Taste Receptmentioning

confidence: 99%

Section: Abstract: Bitter Taste Transduction; Mudpuppy; Taste Receptmentioning

confidence: 99%

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Bitter Taste Transduction of Denatonium in the MudpuppyNecturus maculosus

1997

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“…This process occurs in the apical microvilli of the taste cells, which reside in the gustatory epithelium. The taste molecule binds to its specific receptor and initiates a cascade of molecular events, that finally result in the molecule-specific taste perception [6][7][8][9][10][11][12][13][14][15][16][17]. There are two general types of taste receptors: one, the ion channels and the other, the G-protein coupled seven-transmebrane spanning receptors.…”

Section: Introductionmentioning

confidence: 99%

S100B‐modulated Ca²⁺‐dependent ROS‐GC1 transduction machinery in the gustatory epithelium: a new mechanism in gustatory transduction

Duda¹,

Sharma²

2004

FEBS Letters

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Gustatory transduction is a biochemical process by which the gustatory signal generates the electric signal. The microvilli of the taste cells in the gustatory epithelium are the sites of gustatory transduction. This study documents the biochemical, molecular, and functional identity of the Ca 2+ -modulated membrane guanylate cyclase transduction machinery in the bovine gustatory epithelium. The machinery is a twocomponent system: the Ca 2+ -sensor protein, S100B; and the transducer, ROS-GC1. S100B senses increments in free Ca 2+ , undergoes conformational change, binds to the domain amino acids (aa) Gly962-Asn981 and via the transduction domain aa Ile1030-Gln1041 activates ROS-GC1, generating the second messenger, cyclic GMP. In a recent study, operational presence of this machinery has been demonstrated in the photoreceptor bipolar synapse [Duda et al., EMBO J. 21 (2002) 2547. Thus, the machinery has a broader role in sensory perceptions, vision in the retinal neurons and gustation in the tongue. The entry of the ROS-GC transduction machinery defines the beginning of a new paradigm of Ca 2+ signaling in the tongue.

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Olfaction and Taste

Lorenzo

Youngentob

2003

Handbook of Psychology

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The ability to detect and interpret the chemicals in our environment affects nearly every aspect of our survival. The flavor of our food, i.e., the combination of its taste and smell, is used both to prompt ingestion of nutrients and to protect us from ingesting or inhaling toxins. In the study of taste and olfaction, there are certain as yet unresolved questions that date from the very earliest research into these areas. For example, there is continued debate on the nature of primary stimuli, on the mechanisms of transduction, and on the ways in which stimuli are encoded and interpreted by the nervous system. In this review, the current thinking about these questions, as well as some recent data that may help to resolve them, are outlined. Data from both animal and human models are used to show how technological advances, particularly in the realm of molecular neurobiology, are providing the tools for a new and more complete understanding of how we taste and smell the chemicals in our environment.

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Generation of inositol phosphates in bitter taste transduction

Cited by 75 publications

References 22 publications

Bitter Taste Transduction of Denatonium in the MudpuppyNecturus maculosus

Bitter Taste Transduction of Denatonium in the MudpuppyNecturus maculosus

S100B‐modulated Ca²⁺‐dependent ROS‐GC1 transduction machinery in the gustatory epithelium: a new mechanism in gustatory transduction

Olfaction and Taste

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Generation of inositol phosphates in bitter taste transduction

Cited by 75 publications

References 22 publications

Bitter Taste Transduction of Denatonium in the MudpuppyNecturus maculosus

Bitter Taste Transduction of Denatonium in the MudpuppyNecturus maculosus

S100B‐modulated Ca2+‐dependent ROS‐GC1 transduction machinery in the gustatory epithelium: a new mechanism in gustatory transduction

Olfaction and Taste

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Product

Resources

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S100B‐modulated Ca²⁺‐dependent ROS‐GC1 transduction machinery in the gustatory epithelium: a new mechanism in gustatory transduction