Generation of microalga Chlamydomonas reinhardtii expressing VP28 protein as oral vaccine candidate for shrimps against White Spot Syndrome Virus (WSSV) infection

Lanh, Pham Thi; Nguyen, Huong Minh; Hoa, Nguyễn Thị; Thom, Le Thi; Tam, Luu Thi; Thu, Hà Thị; Nha, Vo Van; Hồng, Đặng Diễm; Mouradov, Aidyn; Koyande, Apurav Krishna; Show, Pau Loke; Quyền, Đồng Văn

doi:10.1016/j.aquaculture.2021.736737

Cited by 24 publications

(12 citation statements)

References 50 publications

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“…Scholars have successfully expressed VP28 in C. reinhardtii and tested its efficacy with herring infected with the white spot syndrome virus (WSSV). The survival rate of shrimp fed transgenic C. reinhardtii was significantly higher than that fed the wild type [ 44 ]. This shows that the antibacterial effect of antimicrobial peptides can still be achieved by directly feeding the algae expressing antimicrobial peptide genes, and it can also provide abundant nutrients.…”

Section: Discussionmentioning

confidence: 99%

Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of Porphyridium purpureum Possessed Antibacterial Properties

Han

Zhao²,

Liu

et al. 2022

Marine Drugs

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To solve the problem of antibiotic abuse in aquaculture and to utilize the application potential of antimicrobial peptides (AMPs), a chloroplast transformation system of Porphyridium purpureum was successfully constructed for effectively expressing two exogenous AMPs. The endogenous fragments of 16S rDNA/trnA-23S rDNA were used as flanking fragments for the homologous recombination in the chloroplast genome. Two AMPs encoded by the transformation vector were controlled by the native promoter psbB in a polycistron. The plasmids were transferred into P. purpureum via particle bombardment and the transformation vectors were screened using phosphinothricin (bar), a dominant selection marker under the control of the psbA promoter. Subsequently, in the positive transformed colonies, the exogenous fragments were found to be inserted in the flanking fragments directionally as expected and two foreign AMPs were successfully obtained. Finally, two exogenous peptides with antibacterial properties were obtained from the transformed strain. The two AMPs expressed by the transformed strain were shown to have similar inhibitory effects to antibiotics by inhibition tests. This suggested that AMPs can be introduced into aquaculture using baited microalgae, providing new ideas and ways to solve a series of aquaculture diseases caused by bacteria.

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Section: Discussionmentioning

confidence: 99%

Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of Porphyridium purpureum Possessed Antibacterial Properties

Han

Zhao²,

Liu

et al. 2022

Marine Drugs

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“…Chlamydomonas reinhardtii is a model eukaryotic microalgal species widely studied with regard to synthetic biology and genetic engineering. For example, feeding engineered C. reinhardtii expressing the foreign VP28 protein to shrimp significantly controlled its white spot disease (Pham et al, 2021). In addition, the xylulose reductase derived from Neurospora crassa was codon‐optimized and transferred into C. reinhardtii to obtain a xylitol yield of 0.38 g/L (Pourmir et al, 2013).…”

Section: High Value‐added Compoundsmentioning

confidence: 99%

Converting carbon dioxide to high value‐added products: Microalgae‐based green biomanufacturing

et al. 2023

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“…The D. salina genome was effectively converted with the hepatitis B virus surface antigen gene (Soria-Guerra et al 2014). The viral envelop protein gene VP28 for White Spot Syndrome Virus (WSSV) was successfully transformed into the nuclear genome of C. reinhardtii resulting in recombinant C. reinhardtii providing a suitable candidate for oral vaccine against this WSSV (Lanh et al 2021).…”

Section: Subunit Vaccinementioning

confidence: 99%

Transformation and Expression of Gene in Microalgae: From Tools to Applications

Usmaan¹,

Rao²

2021

Agrobiol. Records

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Microalgae provides an excellent platform for foreign genes expression. Chloroplast and Nucleus based transformation of transgenes in algae is possible after construct designing (including different markers and promoters) and codon optimization. For better recombination, different and effective promoters and reporter genes can be selected. Chloroplast mediated transformation is much more effective than nuclear based transformation for transgene analysis owing to higher copy number of chloroplast in unit area. Microalgae based products are excellent target for production of wide variety of bio products such as vaccines, antibiotics and drugs against different diseases. These alternative approaches facilitate enhanced biodiesel production through fast growth of microalgae in heterotrophic environment. Also, low risks are attributed owing to their nonhuman consumption property. Biosafety and risk assessment analysis should be carried out before releasing of genetically modified microalgae in the environment considering research ethical code. Microalgae provide cheap, efficient and high yielding alternatives to plants. The review emphasizes microalgae based expression approach with key steps involved in detail.

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Generation of microalga Chlamydomonas reinhardtii expressing VP28 protein as oral vaccine candidate for shrimps against White Spot Syndrome Virus (WSSV) infection

Cited by 24 publications

References 50 publications

Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of Porphyridium purpureum Possessed Antibacterial Properties

Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of Porphyridium purpureum Possessed Antibacterial Properties

Converting carbon dioxide to high value‐added products: Microalgae‐based green biomanufacturing

Transformation and Expression of Gene in Microalgae: From Tools to Applications

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