Generation of microfluidic gradients and their effects on cells behaviours

Tang, Qiangqiang; Yang, Xiran; Xuan, Chengkai; Wu, Kai; Lai, Chen; Shi, Xuetao

doi:10.1007/s42242-020-00093-5

Cited by 4 publications

(2 citation statements)

References 28 publications

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“…Therefore, the cell migration ratio on the IMHCS was higher than the CS and HAS. Apart from the mechanical properties of the scaffold, the topography of the scaffold can also affect the cell migration ratio [35,36]. The directly added nano-HA particles accumulated in the HAS which caused a more rugged and complex topography, and the aggregated particles increased the pores size that further created a craggy surface in the HAS.…”

Section: Discussionmentioning

confidence: 99%

In-situ mineralized homogeneous collagen-based scaffolds for potential guided bone regeneration

Du¹,

Gao²,

Tang³

et al. 2022

Biofabrication

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For guided bone regeneration (GBR) in clinical orthopedics, the importance of a suitable scaffold which can provide the space needed for bone regeneration and simultaneously promotes the new bone formation cannot be overemphasized. Due to its excellent biocompatibility, mechanical strength, and similarity in structure and composition to natural bone, the mineralized collagen-based scaffolds have been increasingly considered as promising GBR scaffolds. Herein, we propose a novel method to fabricate an in-situ mineralized homogeneous collagen-based scaffold (IMHCS) with excellent osteogenic capability for GBR by electrospinning the collagen solution in combination with essential mineral ions. The IMHCS exhibited homogeneous distribution of apatite crystals in electrospun fibers, which helped to achieve a significantly higher tensile strength than the pure collagen scaffold (CS) and the scaffold with directly added nano-hydroxyapatite particles (HAS). Furthermore, the IMHCS had significantly better cell compatibility, cell migration ratio, and osteogenic differentiation property than the HAS and CS. Therefore, the IMHCS not only retains traditional function of inhibiting fibroblast invasion, but also possesses excellent osteogenic differentiation property, indicating a robust alternative for GBR applications.

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Section: Discussionmentioning

confidence: 99%

In-situ mineralized homogeneous collagen-based scaffolds for potential guided bone regeneration

Du¹,

Gao²,

Tang³

et al. 2022

Biofabrication

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“…Recently, significant progress has been made on microfluidic-based EV isolation approaches, which offer improved separation efficiency, reduced processing time, and the ability to meet sample volume requirements [29,30]. Previously, the laminar and uniaxial flow in conventional planar substrate-based microfluidic devices commonly resulted in limited interaction between the particles and the device surface [31,32]. Today, non-planar microfluidic devices with incorporated nanostructure substrates have been developed and exhibit promising EV capture performance.…”

Section: Introductionmentioning

confidence: 99%

Recent progress on microfluidic devices with incorporated 1D nanostructures for enhanced extracellular vesicle (EV) separation

et al. 2022

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A review of recent progress toward the efficient separation of circulating tumor cells via micro‐/nanostructured microfluidic chips

Liu

Yan

Aazmi

et al. 2022

VIEW

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Circulating tumor cells (CTCs) are closely correlated with cancer diagnoses, and their early detection can help patients receive accurate prognoses in a noninvasive manner. However, the rarity, fragility, and heterogeneity of CTCs introduce significant technical challenges to their separation from blood. Recent advances in micro‐/nanofabrication techniques have made microfluidic chips better for CTC separation. In this review, we mainly focus on recent progress made in the development of microfluidic chips incorporating micro‐/nanostructures for the CTC separation. Specifically, we reviewed two major CTC separation mechanisms, that is, physical and chemical approaches. Representative works on physical mechanism‐based approaches (include pillar filtration, cross‐flow filtration, deterministic lateral displacement, smart materials, and bionics); immunomagnetic beads, and surface modification; and chemical mechanism‐based approaches include diversity of affinity ligand selection, are reviewed. Their advantages and disadvantages are compared and discussed, and a perspective on the future direction of CTC is provided.

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Generation of microfluidic gradients and their effects on cells behaviours

Cited by 4 publications

References 28 publications

In-situ mineralized homogeneous collagen-based scaffolds for potential guided bone regeneration

In-situ mineralized homogeneous collagen-based scaffolds for potential guided bone regeneration

Recent progress on microfluidic devices with incorporated 1D nanostructures for enhanced extracellular vesicle (EV) separation

A review of recent progress toward the efficient separation of circulating tumor cells via micro‐/nanostructured microfluidic chips

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