Generation of mutant lines of Nigella sativa L. by induced mutagenesis for improved seed yield

The morphological and phytochemical studies on the effect of acute and recurrent irradiation in Celosia cristata seeds

“…Recently, mutation breeding in the plant has been widely used to create new plant varieties ( Zhou et al 2015;Oladosu et al 2016;Jankowicz-Cieslak et al 2017). Gamma-ray irradiation is one alternative technique that advantageous (e.g., rapid, effective) to create plant mutants that potential for breeding varieties ( Atay et al 2018;Asif and Khalil Ansari 2019;Parchin et al 2019). Recent studies have been successfully recording the use of gamma rays in improving morphology and related character changes, such as Nigella sativa (Asif and Khalil Ansari 2019), Abelmoschus esculentus (Amir et al 2018), C. cristata plantlets (Muhallilin et al 2019, and apple (Atay et al 2018).…”

“…Gamma-ray irradiation is one alternative technique that advantageous (e.g., rapid, effective) to create plant mutants that potential for breeding varieties ( Atay et al 2018;Asif and Khalil Ansari 2019;Parchin et al 2019). Recent studies have been successfully recording the use of gamma rays in improving morphology and related character changes, such as Nigella sativa (Asif and Khalil Ansari 2019), Abelmoschus esculentus (Amir et al 2018), C. cristata plantlets (Muhallilin et al 2019, and apple (Atay et al 2018). Yamaguchi (1988) has found radiosensitivity of gamma-ray irradiation in C. cristata, in which the LD50 value is 480 Gy.…”

Proteome Profiling of the Mutagen-Induced Morphological and Yield Macro-Mutant Lines of Nigella sativa L.

“…Improvement of medicinal plants to give them higher yields and more active ingredients through induced mutagenesis has been reported over the last several years, though these represent only 0.2% of the total crop varieties developed through mutation breeding [3]. In our earlier study, Nigella sativa L. was treated with ethyl methane sulfonate, γ-rays, and a combination of treatments to explore mutagen-induced genetic variability using chemical and physical mutagens; this study reported nine lines of mutants [15,16]. These mutant plants showed significant variability in their growth habits, shapes, and the colors of their leaves; the characteristics of their flowers and seeds; their seed thymoquinone content; and their seed yield [15].…”

“…In our earlier study, Nigella sativa L. was treated with ethyl methane sulfonate, γ-rays, and a combination of treatments to explore mutagen-induced genetic variability using chemical and physical mutagens; this study reported nine lines of mutants [15,16]. These mutant plants showed significant variability in their growth habits, shapes, and the colors of their leaves; the characteristics of their flowers and seeds; their seed thymoquinone content; and their seed yield [15]. Induced mutagenesis has many advantages because of its easy processing and requirement of little genetic information from the plant.…”

“…Since the production of black cumin is limited and the demand for seeds and fixed oil is increasing, the development of genetically improved N. sativa is needed so that future demand for this plant can be fulfilled. Since a high genetic similarity has been reported among populations of N. sativa [13,14], we have developed genetically variable mutant lines of this plant with contrasting morphological traits, seed yields, and thymoquinone content through induced mutagenesis [15]. Because our horizons of research are expanding through genomic, proteomic, and metabolomic approaches, thereby offering opportunities to explore significant information on medicinal plants, identification of the proteins involved in the expression of traits in these mutant lines might help elucidate the metabolic pathways involved in the expression of characters, including seed yield and thymoquinone content.…”