Generation of new human embryonic stem cell lines with diploid and triploid karyotypes

Baharvand, Hossein; Ashtiani, S Kazemi; Taee, A; Massumi, Mohammad; Valojerdi, Mojtaba Rezazadeh; Yazdi, P Eftekhari; Moradi, Sharif; Farrokhi, Ali

doi:10.1111/j.1440-169x.2006.00851.x

Cited by 139 publications

(91 citation statements)

References 38 publications

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“…Colonies were cultured in hESC medium (with KOSR and bFGF) in the absence of feeders and were propagated with enzymatic protocols. The calculated population doubling time of hiPSC was 31.7 ± 3.9 hours (hiPSC1) hours which is comparable to levels similar to those in hESCs (Baharvand et al, 2006a).…”

Section: Characterization Of Established Hipscssupporting

confidence: 49%

Feeder- and serum-free establishment and expansion of human induced pluripotent stem cells

Totonchi¹,

Taei²,

Seifinejad³

et al. 2010

Int. J. Dev. Biol.

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Although human induced pluripotent stem cells (hiPSCs) hold great promise as a source of differentiated cells for vast therapeutic implications, many obstacles still need to be surmounted before this can become a reality. One obstacle, a robust feeder-and serum-free system to generate and expand hiPSCs in culture is still unavailable. Here, for the first time, we describe a novel establishment and maintenance culture technique that uses human dermal fibroblasts to generate hiPSCs by introducing four factors, Klf4, Oct4, Sox2, and c-Myc under serum-and feeder-independent conditions. We have used a serum replacement product, conditioned medium (CM), or feeder-free medium (FFM) supplemented with high elevated basicfibroblast growth factor in the absence or presence of Matrigel. Our FFM system in the presence of Matrigel enhanced the efficiency of alkaline phosphatase-positive colonies at a frequency at least 10-fold greater than the conventional method on feeder cells. The established hiPSCs are similar to human embryonic stem cells in many aspects including morphology, passaging, surface and pluripotency markers, normal karyotype, gene expression, ultrastructure, and in vitro differentiation. Such hiPSCs could be useful particularly in the context of in vitro disease modeling, pharmaceutical screening and in cellular replacement therapies once the safety issues have been overcome.

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Section: Characterization Of Established Hipscssupporting

confidence: 49%

Feeder- and serum-free establishment and expansion of human induced pluripotent stem cells

Totonchi¹,

Taei²,

Seifinejad³

et al. 2010

Int. J. Dev. Biol.

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“…Triploid hES cells have been derived from diploid or triploid blastocysts. 11,40 These hES cells share characteristics such as self-renewal and multi-differentiation competence with diploid hES cells, which is in accordance with our results.…”

Section: Discussionsupporting

confidence: 91%

Diploid, but not haploid, human embryonic stem cells can be derived from microsurgically repaired tripronuclear human zygotes

Fan

Huang

et al. 2013

Cell Cycle

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“…To use hESCs in cell-based therapies, it is necessary to establish many cell lines, as each line may have its own characteristics and advantages for different applications. Furthermore, to overcome the immune rejection of hESCs after transplantation, it was proposed to establish a stem cell bank that contains hundreds of thousands of immunophenotyped stem cell lines to cover the vast spectrum of transplant antigens and to avoid rejection [19,20]. Although recent reports suggest that hESCs are derived more efficiently from high-quality embryos, it is difficult to obtain good-quality human embryos for hESC derivation [21,22].…”

Section: In Vitro and In Vivo Differentiation Potential Of The Hesc Linementioning

confidence: 99%

Triploid and diploid embryonic stem cell lines derived from tripronuclear human zygotes

Chen

Luo

Fan

et al. 2012

J Assist Reprod Genet

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Purpose Human embryonic stem cells (hESCs) are selfrenewing, pluripotent cells that are valuable research tools and hold promise for use in regenerative medicine. The need for new hESC lines motivated our attempts to find a new resource for the derivation of hESC lines. The aim of this work was to establish more hESC lines from abnormal fertilized zygotes and to meet the emerging requirements for their use in cell replacement therapies, disease modeling, and basic research. Methods A total of 130 tripronuclear human zygotes was collected 18-20 h post-insemination and cultured in a modified culture medium. The inner cell mass of 12 blastocysts were isolated by a mechanical method in order to establish embryonic stem cell lines. Results We established four hESC lines derived from 130 trinuclear zygotes, one of which was triploid and the others were diploid. The efficiency of deriving hESC lines is 3.08%. The ratio of deriving triploid and diploid hESC lines is 1:3. All of these hESC lines exhibited similar markers of undifferentiated hESCs and had the typical morphology of hESCs, a capacity for long-term proliferation, and pluripotent differentiation potential both in vivo and in vitro.Conclusions These abnormal zygotes, which otherwise would have been discarded, can serve as an alternative source for normal euploid hESC lines.

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Generation of new human embryonic stem cell lines with diploid and triploid karyotypes

Cited by 139 publications

References 38 publications

Feeder- and serum-free establishment and expansion of human induced pluripotent stem cells

Feeder- and serum-free establishment and expansion of human induced pluripotent stem cells

Diploid, but not haploid, human embryonic stem cells can be derived from microsurgically repaired tripronuclear human zygotes

Triploid and diploid embryonic stem cell lines derived from tripronuclear human zygotes

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