Generation of Novel Thyroid Cancer Stem-Like Cell Clones

Hardin, Heather; Yu, Xinhua; Harrison, April D.; Larraín, C; Zhang, Ranran; Chen, Jidong; Chen, Herbert; Lloyd, Ricardo V.

doi:10.1016/j.ajpath.2016.02.003

Cited by 28 publications

(42 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mice were injected with 1 × 10 4 cells and the tumors were harvested after 8 weeks. 37 The tumor cells show aggressive phenotype with large nuclei, prominent mitotic activity (arrow) and focal areas of necrosis (data not shown). (Hematoxylin and eosin; scale bar, 100 μm).…”

Section: Role Of the Microenvironment And Exosomes In Emt And Cscsmentioning

confidence: 99%

“…[99][100][101] These molecules can be internalized directly by stromal and Figure 3 (a) Clonogenic spheres derived from a single anaplastic thyroid carcinoma cell as previously reported. 37 The cells were separated into ALDEFLUOR-positive and -negative cells by flow cytometry and cell sorting. The ALDEFLUOR-positive cells formed spheres in serum-free media supplemented with EGF and FGF, 36 but the ALDEFLUOR-negative cells did not (scale bar, 50 μm).…”

Section: Role Of the Microenvironment And Exosomes In Emt And Cscsmentioning

confidence: 99%

“…These findings supported the earlier report of Buehler et al 35 showing that primary thyroid carcinoma specimens underwent EMT during thyroid cancer progression from PTCs to ATCs. The studies of Hardin et al 36,37 showed that when thyroid tumor cell lines, including well-differentiated PTC cell lines, as well as ATC cell lines, undergo EMT, there is also an increase in the number of stem cells in the tumor population. Similar findings were reported in colorectal cancer under the influence of Notch-1 expression.…”

mentioning

confidence: 99%

“…4,5,12,18,23,25,28,31,36,37,39,40,[108][109][110] Figure 1 Diagrammatic illustration of epithelial to mesenchymal transition (EMT) and interaction of cancer stem cells (CSCs) with the tumor microenvironment. EMT is influenced by multiple factors and TGFβ-induced EMT is a useful experimental model.…”

mentioning

confidence: 99%

“…25 Similar findings have been shown in the thyroid. [34][35][36][37] Hardin et al 36,37 used a model of cultured thyroid cancer cells treated with TGFβ1, which led to the development of EMT. These findings supported the earlier report of Buehler et al 35 showing that primary thyroid carcinoma specimens underwent EMT during thyroid cancer progression from PTCs to ATCs.…”

mentioning

confidence: 99%

See 4 more Smart Citations

The evolving concept of cancer stem-like cells in thyroid cancer and other solid tumors

Hardin

Zhang

Helein

et al. 2017

Laboratory Investigation

Self Cite

View full text Add to dashboard Cite

The cancer stem-like cell (CSC) hypothesis postulates that a small population of cells in a cancer has self-renewal and clonal tumor initiation properties. These cells are responsible for tumor initiation, growth, recurrence and for resistance to chemotherapy and radiation therapy. CSCs can be characterized using markers such as SSEA-1, SSEA-4, CD44, CD24, ALDEFLUOR and others. CSCs form spheres when they are cultured in serum-free condition in low attachment plates and can generate tumors when injected into immune-deficient mice. During epithelial to mesenchymal transition (EMT), cells lose cellular adhesion and polarity and acquire an invasive phenotype. Recent studies have established a relationship between EMT and increased numbers of CSCs in some solid malignancies. Non-coding RNAs such as microRNAs and long non-coding RNAs (lncRNAs) have been shown to have important roles during EMT and some of these molecules also have regulatory roles in the proliferation of CSCs. Specific lncRNAs enhanced cell migration and invasion in breast carcinomas, which was associated with the generation of stem cell properties. The tumor microenvironment of CSCs also has an important role in tumor progression. Recent studies have shown that the interaction between tumor cells and the local microenvironment at the metastatic site leads to the development of premetastatic niche(s) and allows for the proliferation of the metastatic cells during colonization. The role of exosomes in the microenvironment during the EMT program is currently a major area of research. This review examines CSCs and the relationship between EMT and CSCs in solid tumors with emphasis on thyroid CSCs. The role of non-coding RNAs and of the microenvironment in EMT and in tumor progression are also examined. This review also highlights the growing number of studies that show the close association of EMT and CSCs and the role of exosomes and other elements of the tissue microenvironment in CSC metastasis. A better understanding of these mechanisms will lead to more effective targeting of primary and metastatic malignancies.

show abstract

Section: Role Of the Microenvironment And Exosomes In Emt And Cscsmentioning

confidence: 99%

Section: Role Of the Microenvironment And Exosomes In Emt And Cscsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

The evolving concept of cancer stem-like cells in thyroid cancer and other solid tumors

Hardin

Zhang

Helein

et al. 2017

Laboratory Investigation

Self Cite

View full text Add to dashboard Cite

show abstract

Targeting cancer stem cells and signaling pathways by resveratrol and pterostilbene

Zhang

Wen

et al. 2017

BioFactors

View full text Add to dashboard Cite

In past decades, increasing evidence regarding cancer stem cells (CSCs) may account for carcinogenesis, tumor drug-resistant, and metastasis. CSCs are even considered as the root causes of tumor recurrence and metastases. Targeting CSCs may provide a new clue to cure cancer. Epidemiological and clinical studies have suggested that intake of dietary natural products may bring health benefits including lowering risk of cancer incidence. In this review, we have particularly focused on targeting signaling pathways of CSCs by natural resveratrol and its dimethylated derivative pterostilbene. © 2017 BioFactors, 44(1):61-68, 2018.

show abstract