Generation of seed lipoxygenase-free soybean using CRISPR-Cas9

Wang, Jie; Kuang, Huaqin; Zhang, Zhihui; Yang, Yongqing; Long, Yan; Zhang, Mengchen; Song, Shanshan; Guan, Yuefeng

doi:10.1016/j.cj.2019.08.008

Cited by 95 publications

(58 citation statements)

References 35 publications

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“…Bao et al (2019) used a CRISPR/Cas9‐based multiplex genome‐editing tool to mutate four GmSPL9 genes, and created various soybean mutants containing different combinations of mutated loci, which showed altered node number on the main stem and branch number. Seed related traits, such as seed oil profile (Do et al, 2019), unpleasant beany flavor of soybean seed product (Wang, Kuang, et al, 2020), and isoflavone content and resistance to soybean mosaic virus (Zhang et al, 2020), have been improved by CRISPR/Cas9 technology. In addition, Li et al (2019) tested gRNAs for generating mutants of seed storage protein genes, which will be useful for breeding food‐type soybeans.…”

Section: Crispr/cas9 For Crop Improvement In Legumesmentioning

confidence: 99%

CRISPR/Cas9 gene editing in legume crops: Opportunities and challenges

et al. 2021

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Legumes are an excellent source of proteins and health‐promoting phytochemicals. Recognizing their importance in human nutrition and sustainable agricultural production, significant efforts are currently being made to accelerate genetic gain related to yield, stress resilience, and nutritional quality. Recent increases in genomic resources for multiple legume crops have laid a solid foundation for application of transformative breeding technologies such as genomic selection and genome editing for crop improvement. In this review, we focus on the recent plant‐specific advances in CRISPR/Cas9‐based gene editing technology and discuss the challenges and opportunities to harnessing this innovative technology for targeted improvement of traits in legume crops. Gene‐editing methods have been successfully established for soybean, cowpea, chickpea, and model legumes such as Medicago truncatula and Lotus japonicus. However, the recalcitrance of other legumes to in vitro gene transfer and regeneration has posed a serious challenge to application of gene editing. We discuss various modifications to in vitro culture methods, in terms of the choice of explant, media composition, and DNA delivery and gene‐editing detection methods that can potentially improve the rate of transformation and regeneration of whole plant in legume crops. Although gene‐editing technology can bring enormous benefits to legume breeding, regulatory hurdles are a cause for serious concern. We compare the regulatory environments existing in the European Union and the United States of America. A favorable regulatory framework and public acceptance are important factors in realizing CRISPR's potential benefits to global food security.

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Section: Crispr/cas9 For Crop Improvement In Legumesmentioning

confidence: 99%

CRISPR/Cas9 gene editing in legume crops: Opportunities and challenges

et al. 2021

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“…PCR/RE assay detection method was used frequently in soybean (Michno et al, 2015;Sun et al, 2015;Kanazashi et al, 2018;Di et al, 2019). T7EI assay was occasionally used (Cai et al, 2015;Du et al, 2016) and PCR/Sequencing assay is now the most popular method used to detect mutation in soybean (Haun et al, 2014;Campbell et al, 2019;Cheng et al, 2019;Do et al, 2019;Han et al, 2019;Li R. et al, 2019;Wang J. et al, 2020). PCR/RE and T7EI are cost efficient method when large number putative mutations need to be screened, but limitation of restriction enzyme cut sites can restrict design of sgRNA.…”

Section: Screening Of Mutation Events Created By Ge (Figures 2l-m)mentioning

confidence: 99%

“…Moreover, the percentage of protein in the seeds was increased from 37.52% to 40.58% (Wu et al, 2020). Seed lipoxygenasefree soybean was created by mutating three lipoxygenases genes (LOXs, including LOX1, LOX2, and LOX3) using CRISPR/Cas9 since beany flavor restricts human consumption of soybean (Wang J. et al, 2020). Soybean is a short-day (SD) plant and it tends to flower when the day length reduces to a certain extent.…”

Section: Modification Of Agronomy Traitsmentioning

confidence: 99%

“…Early flowing mutations under natural longday (NLD) conditions was also created by knockout E1gene (Han et al, 2019) and GmPRR37 encoding a pseudo-response regulator protein which is related to photoperiod sensitivity using CRISPR system (Wang L. et al, 2020). These flowering time related mutations can be consistently inherited in next generation (Cai et al, 2018;Han et al, 2019;Cai et al, 2020a;Wang J. et al, 2020) and will be a useful resource for developing elite soybean varieties in the future. Plant architecture can be modified for improving yield.…”

Section: Modification Of Agronomy Traitsmentioning

confidence: 99%

“…For SDN1 mutations in soybean, Agrobacterium-mediated transformation system is currently an efficient and popular method (Table 2) due to convenient mature seed used as explants. GE with multiplex have been achieved in soybean to recover simultaneously target multiple genes or genomic sites in a single transformation event through either making one construct with the expression of multiple sgRNAs using a single Cas9 (Bao et al, 2019;Do et al, 2019) or infecting target tissue with pooled Agrobacterium strains each containing one sgRNA (Kanazashi et al, 2018;Cheng et al, 2019;Do et al, 2019;Wang J. et al, 2020;Bai et al, 2020). Biolistic delivery plus embryogenesis-based regeneration system is an alternative method to recover GE evens with various outcomes.…”

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confidence: 99%

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Progresses, Challenges, and Prospects of Genome Editing in Soybean (Glycine max)

Zhang

et al. 2020

Front. Plant Sci.

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Soybean is grown worldwide for oil and protein source as food, feed and industrial raw material for biofuel. Steady increase in soybean production in the past century mainly attributes to genetic mediation including hybridization, mutagenesis and transgenesis. However, genetic resource limitation and intricate social issues in use of transgenic technology impede soybean improvement to meet rapid increases in global demand for soybean products. New approaches in genomics and development of site-specific nucleases (SSNs) based genome editing technologies have expanded soybean genetic variations in its germplasm and have potential to make precise modification of genes controlling the important agronomic traits in an elite background. ZFNs, TALENS and CRISPR/Cas9 have been adapted in soybean improvement for targeted deletions, additions, replacements and corrections in the genome. The availability of reference genome assembly and genomic resources increases feasibility in using current genome editing technologies and their new development. This review summarizes the status of genome editing in soybean improvement and future directions in this field.

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