Generation of the viral nerve necrosis in kelpbass and tigar globefishes.

Nakai, Tadaomi; Dung, Nguyen Huu; Nishizawa, Tsutomu; Muroga, Kiyokuni; Arimoto, Masao; Ootsuki, K.

doi:10.3147/jsfp.29.211

Cited by 52 publications

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“…The SJNNV-type strains have been detected in Japan and the Iberian Peninsula, both in farmed species and in wild fish populations (Bandín and Souto 2020). Finally, the TPNNV group includes a unique NNV isolate obtained in Japan from Tiger Puffer Takifugu rubripes (Nakai et al 1994). Natural reassortment between RGNNV and SJNNV genomic segments was reported by Toffolo et al (2007); since then, several reassortants have been detected in fish from southern Europe, including Senegalese Sole Solea senegalensis, Gilthead Seabream Sparus aurata, European Seabass Dicentrarchus labrax, and European Pilchard Sardina pilchardus.…”

Section: Introductionmentioning

confidence: 99%

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Detection of different Betanodavirus genotypes in wild fish from Spanish Atlantic coastal waters (Galicia, northwestern Spain)

Vázquez‐Salgado,

Olveira,

Dopazo

et al. 2023

J Aqua Anim Hlth

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The Nervous Necrosis Virus (NNV) is an aquatic pathogen responsible for a neurological disease affecting marine fish. Despite its almost worldwide distribution, global warming could favour NNV spreading towards new areas, highlighting the importance of conducting epidemiological surveys on both wild and farmed marine fish species. In this study we have assessed NNV prevalence in wild fish caught along the Galician Atlantic coast. A total of 1,277 fish were analysed by reverse transcription real‐time PCR (RT‐rtPCR), of which 1.72% tested positive for NNV, including two species in which the pathogen had not yet been reported. The reassortant RGNNV/SJNNV was detected in 55% of these individuals, while the remaining 45% harboured SJNNV type genome. Moreover, 4 reassortant strains were isolated from pilchard and mackerel which carry amino acid mutations at key sites related to NNV‐host interaction.

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Section: Introductionmentioning

confidence: 99%

“…Finally, the TPNNV group includes a unique NNV isolate obtained in Japan from Tiger Puffer Takifugu rubripes (Nakai et al. 1994). Natural reassortment between RGNNV and SJNNV genomic segments was reported by Toffolo et al.…”

Section: Introductionmentioning

confidence: 99%

Detection of different Betanodavirus genotypes in wild fish from Spanish Atlantic coastal waters (Galicia, northwestern Spain)

Vázquez‐Salgado,

Olveira,

Dopazo

et al. 2023

J Aqua Anim Hlth

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Fish Pathology

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Evidence for and characterization of nervous necrosis virus infection in Pacific cod (Gadus macrocephalus)

et al. 2015

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A mortality rate higher than 90% was observed in a larva-rearing facility for Pacific cod, Gadus macrocephalus, in China. Larvae showing clinical signs of infection were collected. Initial suspicion of nervous necrosis virus (NNV) infection was confirmed by sequencing, absolute quantification real-time PCR (A-qPCR), and electron microscopy. The nucleotide sequence of RNA2 was 1,375 bases long (GenBank no. KM576685), coding for a single ORF corresponding to the capsid protein from residues 21 to 1034. Phylogenetic analysis of the capsid protein sequence showed that PCNNV belongs to the barfin flounder NNV (BFNVV) genotype. An amino acid sequence alignment revealed 39 differences between the cold- and warm-resistant viral groups, suggesting that PCNNV evolved under temperature selection. The 3-D structure of the predicted capsid protein was modeled to identify potential epitopes, and the gene was expressed in Escherichia coli, yielding a protein with a molecular mass of 55 kDa. During PCNNV outbreaks, the viral copy number was found to reach 10(7) per ng of total RNA, which could be considered the lethal copy number of NNV in cod. The gonads, eggs, fertilized eggs and asymptomatic cod fry were all positive for PCNNV, indicating viral vertical transmission as the main source of the viral load. The amount of virus in the apparent healthy fry or survivors seemed to decrease gradually with development. These results might lead to efficient diagnostic methods to help farmers select NNV-free broodfish for cod breeding.

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Generation of the viral nerve necrosis in kelpbass and tigar globefishes.

Cited by 52 publications

References 0 publications

Detection of different Betanodavirus genotypes in wild fish from Spanish Atlantic coastal waters (Galicia, northwestern Spain)

Detection of different Betanodavirus genotypes in wild fish from Spanish Atlantic coastal waters (Galicia, northwestern Spain)

References

Evidence for and characterization of nervous necrosis virus infection in Pacific cod (Gadus macrocephalus)

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